Cartilage inorganic pyrophosphate elaboration is independent of sulfated glycosaminoglycan synthesis

Lawrence M. Ryan, Indira Kurup, Daniel J. McCarty, Herman S Cheung

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Inorganic pyrophosphate (PPi), a product of glycosaminoglycan synthesis, may be cosecreted with matrix proteoglycan to reach the extracellular site where calcium pyrophosphate dihydrate crystals form. To test this hypothesis, sulfated glycosaminoglycan synthesis by articular cartilage in culture was stimulated or inhibited while the effect on extracellular PPi was measured. When stimulated by 0.8 mM xyloside to increase 35SO4 incorporation (mean ± SEM % of control 183 ± 16, n = 5), PPi accumulation changed little (from 54 ± 6 pmoles/mg to 63 ± 8 pmoles/mg of cartilage wet weight). Inhibition of sulfation with monensin or diethylcarbamazine disproportionately lowered 35SO4 incorporation compared with PPi elaboration. Using 60 mM diethylcarbamazine, PPi production was preserved (105 ± 8% mean ± SEM) compared with control cultures, while sulfation was markedly inhibited (7 ± 1%). This dissociation of sulfate incorporation and PPi secretion indicates that it is not likely that glycosaminoglycan sulfation is the source of the PPi that escapes from chondrocytes to participate in the formation of extracellular crystals.

Original languageEnglish
Pages (from-to)235-240
Number of pages6
JournalArthritis and Rheumatism
Volume33
Issue number2
StatePublished - Feb 1 1990
Externally publishedYes

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A73025
Diethylcarbamazine
Glycosaminoglycans
Cartilage
Calcium Pyrophosphate
Monensin
Articular Cartilage
Proteoglycans
Chondrocytes
Sulfates
Weights and Measures
diphosphoric acid

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Cartilage inorganic pyrophosphate elaboration is independent of sulfated glycosaminoglycan synthesis. / Ryan, Lawrence M.; Kurup, Indira; McCarty, Daniel J.; Cheung, Herman S.

In: Arthritis and Rheumatism, Vol. 33, No. 2, 01.02.1990, p. 235-240.

Research output: Contribution to journalArticle

Ryan, Lawrence M. ; Kurup, Indira ; McCarty, Daniel J. ; Cheung, Herman S. / Cartilage inorganic pyrophosphate elaboration is independent of sulfated glycosaminoglycan synthesis. In: Arthritis and Rheumatism. 1990 ; Vol. 33, No. 2. pp. 235-240.
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