TY - JOUR
T1 - Carnosine protects pancreatic beta cells and islets against oxidative stress damage
AU - Miceli, Vitale
AU - Pampalone, Mariangela
AU - Frazziano, Giovanna
AU - Grasso, Giuseppe
AU - Rizzarelli, Enrico
AU - Ricordi, Camillo
AU - Casu, Anna
AU - Iannolo, Gioacchin
AU - Conaldi, Pier Giulio
N1 - Funding Information:
We thank Dr. A. Natalicchio (University of Bari, Italy) for kindly providing the INS-1E cells and Dr. R. Fiume (University of Bologna, Italy) for kindly providing the MIN6 cells. The authors would also like to thank Warren Blumberg of ISMETT's Language Services Department for his language revision of the manuscript. This work was supported by Italian Ministry of Health project: PON02_00607_3421644 .
Funding Information:
We thank Dr. A. Natalicchio (University of Bari, Italy) for kindly providing the INS-1E cells and Dr. R. Fiume (University of Bologna, Italy) for kindly providing the MIN6 cells. The authors would also like to thank Warren Blumberg of ISMETT's Language Services Department for his language revision of the manuscript. This work was supported by Italian Ministry of Health project: PON02_00607_3421644.
PY - 2018/10/15
Y1 - 2018/10/15
N2 - Islet transplantation is a valid therapeutic option for type 1 diabetes treatment. However, in this procedure one of the major problems is the oxidative stress produced during pancreatic islet isolation. The aim of our study was to evaluate potential protective effects of L-carnosine and its isomer D-carnosine against oxidative stress. We evaluated the carnosine effect on cell growth, cell death, insulin production, and the main markers of oxidative stress in rat and murine stressed beta cell lines as well as in human pancreatic islets. Both isomers clearly inhibited hydrogen peroxide induced cytotoxicity, with a decrease in intracellular reactive oxygen and nitrogen species, prevented hydrogen peroxide induced apoptosis/necrosis, nitrite production, and reduced glucose-induced insulin secretion. In addition, NF-κB expression/translocation and nitrated protein induced in stressed cells was significantly reduced. Furthermore, both isomers improved survival and function, and decreased reactive oxygen and nitrogen species, and nitrite and nitrotyrosine production in human islets cultured for 1, 3, and 7 days. These results seem to indicate that both L and D-carnosine have a significant cytoprotective effect by reducing oxidative stress in beta cell lines and human islets, suggesting their potential use to improve islet survival during the islet transplantation procedure.
AB - Islet transplantation is a valid therapeutic option for type 1 diabetes treatment. However, in this procedure one of the major problems is the oxidative stress produced during pancreatic islet isolation. The aim of our study was to evaluate potential protective effects of L-carnosine and its isomer D-carnosine against oxidative stress. We evaluated the carnosine effect on cell growth, cell death, insulin production, and the main markers of oxidative stress in rat and murine stressed beta cell lines as well as in human pancreatic islets. Both isomers clearly inhibited hydrogen peroxide induced cytotoxicity, with a decrease in intracellular reactive oxygen and nitrogen species, prevented hydrogen peroxide induced apoptosis/necrosis, nitrite production, and reduced glucose-induced insulin secretion. In addition, NF-κB expression/translocation and nitrated protein induced in stressed cells was significantly reduced. Furthermore, both isomers improved survival and function, and decreased reactive oxygen and nitrogen species, and nitrite and nitrotyrosine production in human islets cultured for 1, 3, and 7 days. These results seem to indicate that both L and D-carnosine have a significant cytoprotective effect by reducing oxidative stress in beta cell lines and human islets, suggesting their potential use to improve islet survival during the islet transplantation procedure.
KW - Beta cell line
KW - Carnosine
KW - Diabetes
KW - Oxidative stress
KW - Pancreatic islet transplantation
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U2 - 10.1016/j.mce.2018.02.016
DO - 10.1016/j.mce.2018.02.016
M3 - Article
C2 - 29496567
AN - SCOPUS:85042586527
VL - 474
SP - 105
EP - 118
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
SN - 0303-7207
ER -