Cardiovascular changes in group b streptococcal sepsis in the piglet: Response to indomethacin and relationship to prostacyclin and thromboxane A2

Beatriz Runkle, Ronald N. Goldberg, Murray M. Streitfeld, Martin R. Clark, Elena Buron, Emmalee Setzer, Eduardo Bancalari

Research output: Contribution to journalArticle

92 Scopus citations

Abstract

Seventeen piglets were infected with a continuous intravenous infusion of live group B β-hemolytic streptococci (GBS). Hemodynamic changes were recorded, and blood samples were drawn for measurement of thromboxane B2 (TxB2) (stable metabolite of thromboxane A2) and 6-keto-PGE(1α) (stable metabolite of prostacyclin). Control animals (n = 9) received only bacteria, while treatment animals (n = 8) received indomethacin, 3 mg/kg IV, 15 min after the start of the bacterial infusion. Control animals responded to the bacteria within 15 min with marked elevation in mean pulmonary artery pressure (Ppa) from 15 ± 8 to 39 ± 6 mm Hg and decline in PaO2 from 80 ± 11 to 51 ± 6 mm Hg and cardiac output (CO) from 0.24 ± 0.07 to 0.13 ± 0.07 liters/min/kg. Mean arterial blood pressure (AoP) significantly decreased from baseline value of 95 ± 13 to 51 ± 32 mm Hg by 180 min. In animals treated with indomethacin, these changes were reversed or significantly attenuated. The hemodynamic changes were associated temporally with elevations in plasma concentrations of TxB2 or 6-keto-PGF(1α). In the first 60 min, TxB2 levels in both groups correlated with Ppa (r = 0.72, p < 0.001) and PaO2 (r = -0.60, p < 0.001). A strong negative correlation between TxB2 and CO was observed over the first 180 min (r = -0.73, p < 0.001). There was statistically significant correlation between AoP and 6-keto-PGF(1α) concentration between 60 and 180 min (r = -0.54, p < 0.002). Indomethacin improved the hemodynamic function in this model of GBS sepsis. This improvement was related in part to inhibition of synthesis of thromboxane A2 and prostacyclin.

Original languageEnglish (US)
Pages (from-to)874-878
Number of pages5
JournalPediatric Research
Volume18
Issue number9
DOIs
StatePublished - Sep 1984

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

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