Cardiac myocyte hypertrophy is associated with c-myc protooncogene expression

N. F. Starksen, P. C. Simpson, Nanette Bishopric, S. R. Coughlin, W. M. Lee, J. A. Escobedo, L. T. Williams

Research output: Contribution to journalArticle

203 Citations (Scopus)

Abstract

The mechanism of hormonally induced cell hypertrophy is unknown. Stimulation of cardiac myocytes by α1-adrenergic agents, phorbol esters, and serum induces an increase in the cell size of nondividing cardiac myocytes in primary culture. Expression of the c-myc gene, known to be increased in growth factor-induced cell division, was studied in this model of cell hypertrophy. The α-adrenergic agonist norepinephrine (0.002-20 μM) increased levels of c-myc-encoded mRNA to 10-fold over control levels. This increase was detectable at 30 min, peaked at 2 hr, and returned to baseline by 6 hr after stimulation. The norepinephrine response was abolished by the α1-antagonist terazosin (2 μM) but was not affected by the β-adrenergic antagonist propranolol (2 μM) and was only slightly (25%) attenuated by the α2-adrenergic antagonist yohimbine (2 μM). Serum and the phorbol ester tumor promoter phorbol 12-myristate 13-acetate also enhanced c-myc expression in cardiac myocyte cultures. These findings show that the induction of cardiac myocyte hypertrophy is associated with enhanced expression of the c-myc gene and suggest that hormonally induced cell hypertrophy and cell division share common mechanistic pathways.

Original languageEnglish
Pages (from-to)8348-8350
Number of pages3
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number21
DOIs
StatePublished - Dec 1 1986
Externally publishedYes

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Cardiomegaly
Cardiac Myocytes
Hypertrophy
Terazosin
myc Genes
Adrenergic Antagonists
Phorbol Esters
Cell Division
Norepinephrine
Adrenergic Agonists
Yohimbine
Serum
Cell Size
Propranolol
Carcinogens
Adrenergic Agents
Intercellular Signaling Peptides and Proteins
Acetates
Messenger RNA

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Starksen, N. F., Simpson, P. C., Bishopric, N., Coughlin, S. R., Lee, W. M., Escobedo, J. A., & Williams, L. T. (1986). Cardiac myocyte hypertrophy is associated with c-myc protooncogene expression. Proceedings of the National Academy of Sciences of the United States of America, 83(21), 8348-8350. https://doi.org/10.1073/pnas.83.21.8348

Cardiac myocyte hypertrophy is associated with c-myc protooncogene expression. / Starksen, N. F.; Simpson, P. C.; Bishopric, Nanette; Coughlin, S. R.; Lee, W. M.; Escobedo, J. A.; Williams, L. T.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 21, 01.12.1986, p. 8348-8350.

Research output: Contribution to journalArticle

Starksen, NF, Simpson, PC, Bishopric, N, Coughlin, SR, Lee, WM, Escobedo, JA & Williams, LT 1986, 'Cardiac myocyte hypertrophy is associated with c-myc protooncogene expression', Proceedings of the National Academy of Sciences of the United States of America, vol. 83, no. 21, pp. 8348-8350. https://doi.org/10.1073/pnas.83.21.8348
Starksen, N. F. ; Simpson, P. C. ; Bishopric, Nanette ; Coughlin, S. R. ; Lee, W. M. ; Escobedo, J. A. ; Williams, L. T. / Cardiac myocyte hypertrophy is associated with c-myc protooncogene expression. In: Proceedings of the National Academy of Sciences of the United States of America. 1986 ; Vol. 83, No. 21. pp. 8348-8350.
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