Carboxylic acids permeases in yeast: Two genes in Kluyveromyces lactis

Tiziana Lodi, Flavia Fontanesi, Iliana Ferrero, Claudia Donnini

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Two new genes KlJEN1 and KlJEN2 were identified in Kluyveromyces lactis. The deduced structure of their products is typical of membrane-bound carriers and displays high similarity to Jen1p, the monocarboxylate permease of Saccharomyces cerevisiae. Both KlJEN1 and KlJEN2 are under the control of glucose repression mediated by FOG1 and FOG2, corresponding to S. cerevisiae GAL83 and SNF1 respectively, and KlCAT8, proteins involved in glucose signalling cascade in K. lactis. KlJEN1, but not KlJEN2, is induced by lactate. KlJEN2 in contrast is expressed at high level in ethanol and succinate. The physiological characterization of null mutants showed that KlJEN1 is the functional homologue of ScJEN1, whereas KlJEN2 encodes a dicarboxylic acids transporter. In fact, KlJen1p [transporter classification (TC) number: 2.A.1.12.2.] is required for lactate uptake and therefore for growth on lactate. KlJen2p is required for succinate transport, as demonstrated by succinate uptake experiments and by inability of Kljen2 mutant to grow on succinate. This carrier appears to transport also malate and fumarate because the Kljen2 mutant cannot grow on these substrates and the succinate uptake is competed by these carboxylic acids. We conclude that KlJEN2 is the first yeast gene shown to encode a dicarboxylic acids permease.

Original languageEnglish (US)
Pages (from-to)111-119
Number of pages9
JournalGene
Volume339
Issue number1-2
DOIs
StatePublished - Sep 15 2004
Externally publishedYes

Fingerprint

Kluyveromyces
Membrane Transport Proteins
Succinic Acid
Carboxylic Acids
Yeasts
Lactic Acid
Genes
Saccharomyces cerevisiae
Dicarboxylic Acid Transporters
Glucose
Dicarboxylic Acids
Fumarates
Ethanol
Membranes
Growth
Proteins

Keywords

  • BLASTP
  • Carboxylic acids transport
  • GF/C
  • Glasfaser-Circles
  • JEN1
  • JEN2
  • Lactate
  • ORF
  • PCR
  • polymerase chain reaction
  • protein-protein basic local alignment search tool
  • Succinate
  • TC
  • transporter classification
  • Yeast
  • yeast nitrogen base
  • yeast peptone
  • YNB
  • YP

ASJC Scopus subject areas

  • Genetics

Cite this

Carboxylic acids permeases in yeast : Two genes in Kluyveromyces lactis. / Lodi, Tiziana; Fontanesi, Flavia; Ferrero, Iliana; Donnini, Claudia.

In: Gene, Vol. 339, No. 1-2, 15.09.2004, p. 111-119.

Research output: Contribution to journalArticle

Lodi, Tiziana ; Fontanesi, Flavia ; Ferrero, Iliana ; Donnini, Claudia. / Carboxylic acids permeases in yeast : Two genes in Kluyveromyces lactis. In: Gene. 2004 ; Vol. 339, No. 1-2. pp. 111-119.
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