Carbon Nitride Dots: A Selective Bioimaging Nanomaterial

Piumi Y. Liyanage, Regina M. Graham, Raja R. Pandey, Charles C. Chusuei, Keenan J. Mintz, Yiqun Zhou, James K. Harper, Wei Wu, Athula H. Wikramanayake, Steven Vanni, Roger M. Leblanc

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In contrast to the recent immense attention in carbon nitride quantum dots (CNQDs) as a heteroatom-doped carbon quantum dot (CQD), their biomedical applications have not been thoroughly investigated. Targeted cancer therapy is a prominently researched area in the biomedical field. Here, the ability of CNQDs as a selective bioimaging nanomaterial was investigated to assist targeted cancer therapy. CNQDs were first synthesized using four different precursor sets involving urea derivatives, and the characteristics were compared to select the best candidate material for bioapplications. Characterization techniques such as UV-vis, luminescence, X-ray photoelectron spectroscopy, nuclear magnetic resonance spectroscopy, and transmission electron microscopy were used. These CNQDs were analyzed in in vitro studies of bioimaging and labeling using pediatric glioma cells (SJGBM2) for possible selective biolabeling and nanodistribution inside the cell membrane. The in vitro cellular studies were conducted under long-wavelength emission without the interference of blue autofluorescence. Thus, excitation-dependent emission of CNQDs was proved to be advantageous. Importantly, CNQDs selectively entered SJGBM2 tumor cells, while it did not disperse into normal human embryonic kidney cells (HEK293). The distribution studies in the cell cytoplasm indicated that CNQDs dispersed into lysosomes within approximately 6 h after the incubation. The CNQDs exhibited great potential as a possible nanomaterial in selective bioimaging and drug delivery for targeted cancer therapy.

Original languageEnglish (US)
Pages (from-to)111-123
Number of pages13
JournalBioconjugate Chemistry
Volume30
Issue number1
DOIs
StatePublished - Jan 16 2019

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Quantum Dots
Carbon nitride
Nanostructures
Nanostructured materials
Semiconductor quantum dots
Neoplasms
cyanogen
Photoelectron Spectroscopy
Pediatrics
HEK293 Cells
Cell membranes
Luminescence
Lysosomes
Transmission Electron Microscopy
Drug delivery
Urea
Glioma
Labeling
Nuclear magnetic resonance spectroscopy
Tumors

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

Carbon Nitride Dots : A Selective Bioimaging Nanomaterial. / Liyanage, Piumi Y.; Graham, Regina M.; Pandey, Raja R.; Chusuei, Charles C.; Mintz, Keenan J.; Zhou, Yiqun; Harper, James K.; Wu, Wei; Wikramanayake, Athula H.; Vanni, Steven; Leblanc, Roger M.

In: Bioconjugate Chemistry, Vol. 30, No. 1, 16.01.2019, p. 111-123.

Research output: Contribution to journalArticle

Liyanage PY, Graham RM, Pandey RR, Chusuei CC, Mintz KJ, Zhou Y et al. Carbon Nitride Dots: A Selective Bioimaging Nanomaterial. Bioconjugate Chemistry. 2019 Jan 16;30(1):111-123. https://doi.org/10.1021/acs.bioconjchem.8b00784
Liyanage, Piumi Y. ; Graham, Regina M. ; Pandey, Raja R. ; Chusuei, Charles C. ; Mintz, Keenan J. ; Zhou, Yiqun ; Harper, James K. ; Wu, Wei ; Wikramanayake, Athula H. ; Vanni, Steven ; Leblanc, Roger M. / Carbon Nitride Dots : A Selective Bioimaging Nanomaterial. In: Bioconjugate Chemistry. 2019 ; Vol. 30, No. 1. pp. 111-123.
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