Carbon nanotube based biomimetic membranes

Pramod Nednoor, Leonidas G Bachas, Bruce Hinds, Nitin Chopra

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Biological ion channels play a critical role in the transport of fluids and chemicals across cell membranes. Synthetic nanopore membranes can be used to mimic ion channels provided the molecular transport through these membranes is precisely controlled. A major challenge associated with the use of synthetic membranes to mimic biological systems is instilling them with reversible gating properties. Nanotubes/fibers from carbon or inorganic materials can be assembled to construct higher order supramolecular architectures within polymer films/membranes. An aligned array of carbon nanotubes impregnated in a polystyrene matrix can be used as a model system to mimic protein ion channels. Open tips of the carbon nanotubes in this membrane have carboxyl functionality and can be easily derivatized with a molecule that binds to a bulky receptor, which can thus regulate the flow through the pore entrance. A nine residue synthetic peptide containing a serine residue [G-R-T-G-R-R-N-S-I-NH2], which is a specific substrate of Protein Kinase A was functionalized at the tip of carbon nanotubes to obtain a biomimetic system where phosphorylation regulates ligand-gated ion channels. The serine residue was phosphorylated using ATP in the presence of protein kinase. Monoclonal Anti-Phosphoserine antibody (mouse IgG1 isotype) derived from the PSR-45 hybridoma recognizes phosphorylated serine, both as free amino acid or within a peptide. Phosphorylation of the serine residue with a kinase leads to the binding of a monoclonal anti-phosphoserine antibody. This binding event controls the ionic flow through the pores. Dephosphorylating the serine residue with an alkaline phosphatase alters the flow through the channels. The transport of oppositely charged molecules through the CNT membrane was quantified. This work demonstrates possibility of mimicking biological ion channels using ligand induced nanopore structures.

Original languageEnglish (US)
Title of host publicationAIChE Annual Meeting, Conference Proceedings
Pages14149
Number of pages1
StatePublished - 2005
Externally publishedYes
Event05AIChE: 2005 AIChE Annual Meeting and Fall Showcase - Cincinnati, OH, United States
Duration: Oct 30 2005Nov 4 2005

Other

Other05AIChE: 2005 AIChE Annual Meeting and Fall Showcase
CountryUnited States
CityCincinnati, OH
Period10/30/0511/4/05

Fingerprint

Biomimetics
Carbon nanotubes
Membranes
Ions
Phosphorylation
Nanopores
Proteins
Antibodies
Peptides
Ligands
Molecules
Adenosinetriphosphate
Phosphatases
Biological systems
Cell membranes
Polymer films
Nanotubes
Amino acids
Polystyrenes
Carbon

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Nednoor, P., Bachas, L. G., Hinds, B., & Chopra, N. (2005). Carbon nanotube based biomimetic membranes. In AIChE Annual Meeting, Conference Proceedings (pp. 14149)

Carbon nanotube based biomimetic membranes. / Nednoor, Pramod; Bachas, Leonidas G; Hinds, Bruce; Chopra, Nitin.

AIChE Annual Meeting, Conference Proceedings. 2005. p. 14149.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nednoor, P, Bachas, LG, Hinds, B & Chopra, N 2005, Carbon nanotube based biomimetic membranes. in AIChE Annual Meeting, Conference Proceedings. pp. 14149, 05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Cincinnati, OH, United States, 10/30/05.
Nednoor P, Bachas LG, Hinds B, Chopra N. Carbon nanotube based biomimetic membranes. In AIChE Annual Meeting, Conference Proceedings. 2005. p. 14149
Nednoor, Pramod ; Bachas, Leonidas G ; Hinds, Bruce ; Chopra, Nitin. / Carbon nanotube based biomimetic membranes. AIChE Annual Meeting, Conference Proceedings. 2005. pp. 14149
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