Capture and release of viable circulating tumor cells from blood

Siddarth Rawal, Zheng Ao, Ashutosh Agarwal

Research output: Contribution to journalArticle

Abstract

We demonstrate a method for size based capture of viable circulating tumor cell (CTC) from whole blood, along with the release of these cells from chip for downstream analysis and/or culture. The strategy employs the use of a novel Parylene C membrane slot pore microfilter to capture CTC and a coating of poly (N-iso-propylacrylamide) (PIPAAm) for thermoresponsive viable release of the captured CTC. The capture of live cells is enabled by leveraging the design of a slot pore geometry with specific dimensions to reduce the shear stress typically associated with the filtration process. While the microfilter exhibits a high capture efficiency, the release of these cells is non-trivial. Typically, only a small percentage of cells are released when techniques such as reverse flow or cell scraping are used. The strong adhesion of these epithelial cancer cells to the Parylene C membrane is attributable to non-specific electrostatic interaction. To counteract this effect, we employed the use of PIPAAm coating and exploited its thermal responsive interfacial properties to release the cells from the filter. Blood is first filtered at room temperature. Below 32 °C, PIPAAm is hydrophilic. Thereafter, the filter is placed in either culture media or a buffer maintained at 37 °C, which results in the PIPAAm turning hydrophobic, and subsequently releasing the electrostatically bound cells.

Original languageEnglish (US)
Article numbere54435
JournalJournal of Visualized Experiments
Volume2016
Issue number116
DOIs
StatePublished - Oct 28 2016

Keywords

  • CTC culture
  • Cancer research
  • Circulating tumor cell
  • Issue 116
  • Liquid biopsy
  • Live cell capture
  • Microfilter
  • PIPAAm
  • Personalized medicine
  • Precision medicine
  • Thermoresponsive release
  • Translational medicine

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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