Canine RPGRIP1 mutation establishes cone-rod dystrophy in miniature longhaired dachshunds as a homologue of human Leber congenital amaurosis

C. S. Mellersh, M. E.G. Boursnell, L. Pettitt, E. J. Ryder, N. G. Holmes, D. Grafham, O. P. Forman, J. Sampson, K. C. Barnett, S. Blanton, M. M. Binns, M. Vaudin

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

Cone-rod dystrophy 1 (cord1) is a recessive condition that occurs naturally in miniature longhaired dachshunds (MLHDs). We mapped the cord1 locus to a region of canine chromosome CFA15 that is syntenic with a region of human chromosome 14 (HSA14q11.2) containing the retinitis pigmentosa GTPase regulator-interacting protein 1 (RPGRIP1) gene. Mutations in RPGRIP1 have been shown to cause Leber congenital amaurosis, a group of retinal dystrophies that represent the most common genetic causes of congenital visual impairment in infants and children. Using the newly available canine genome sequence we sequenced RPGRIP1 in affected and carrier MLHDs and identified a 44-nucleotide insertion in exon 2 that alters the reading frame and introduces a premature stop codon. All affected and carrier dogs within an extended inbred pedigree were homozygous and heterozygous, respectively, for the mutation. We conclude the mutation is responsible for cord1 and demonstrate that this canine disease is a valuable model for exploring disease mechanisms and potential therapies for human Leber congenital amaurosis.

Original languageEnglish (US)
Pages (from-to)293-301
Number of pages9
JournalGenomics
Volume88
Issue number3
DOIs
StatePublished - Sep 1 2006
Externally publishedYes

Keywords

  • Canine
  • Cone-rod dystrophy
  • Leber congenital amaurosis
  • RPGRIP1

ASJC Scopus subject areas

  • Genetics

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  • Cite this

    Mellersh, C. S., Boursnell, M. E. G., Pettitt, L., Ryder, E. J., Holmes, N. G., Grafham, D., Forman, O. P., Sampson, J., Barnett, K. C., Blanton, S., Binns, M. M., & Vaudin, M. (2006). Canine RPGRIP1 mutation establishes cone-rod dystrophy in miniature longhaired dachshunds as a homologue of human Leber congenital amaurosis. Genomics, 88(3), 293-301. https://doi.org/10.1016/j.ygeno.2006.05.004