Candida rugosa lipase alters the gastrointestinal environment in wild-type mice

Ariane Menden, Davane Hall, Courtney Ann Broedlow, Teresa Darcey, Fiona Crawford, Nichole Klatt, Stefan Crynen, Michael Mullan, Ghania Ait-Ghezala

Research output: Contribution to journalArticlepeer-review


Diet and commercially available supplements can significantly impact the gut microbial composition; however, the effects of supplements often lack scientific data demonstrating the effects on healthy and diseased individuals. Hence, it was investigated, whether a frequently used supplement in humans, Candida rugosa lipase (CRL), gets delivered active beyond the stomach in the intestinal tract of C57BL/6 J mice and its impact on the gut microbial community and environment. We showed for the first time the movement of CRL in an active state through the mouse digestive tract by determination of intestinal CRL activity and free fatty acids concentrations. The short- and long-term administration of CRL resulted in significant alterations of the gut microbiome, favoring the growth of, for instance, Verrucomicrobia but also other species associated with normal body mass index (BMI) or butyrate expression, both considered beneficial. In addition, we showed that these changes persisted after supplementation and that gut barrier integrity was unaffected by the treatment. In conclusion, CRL can be delivered in an active state beyond the stomach and supplementation altered the murine gut microbiome favoring beneficial bacterial species, which may be of relevance in humans in healthy but also potentially in disease states.

Original languageEnglish (US)
Article number110579
JournalBiomedicine and Pharmacotherapy
StatePublished - Oct 2020
Externally publishedYes


  • Akkermansia muciniphila
  • Candida rugosa lipase
  • Enzyme supplement
  • Gut microbiome
  • Intestinal health

ASJC Scopus subject areas

  • Pharmacology


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