TY - JOUR
T1 - Candida albicans nucleoside-diphosphate kinase
T2 - Purification and characterization
AU - Biondi, Ricardo M.
AU - Veron, Michel
AU - Walz, Katherina
AU - Passeron, Susana
N1 - Funding Information:
We are grateful to Dr. Ioan Lascu for a very stimulating exchange of ideas, to Dr. Pedro FernaÂndez-Murray for friendly discussions of our results and to Drs. Ricardo A. Wolosiuk and Jeff Stock for critical reading of the manuscript. This work was supported by grants from the Consejo Nacional de Investigaciones CientÂõ®cas y TeÂcnicas (CON-ICET), FundacioÂn Antorchas, and the International Centre for Genetic Engineering and Biotechnology (ICGEB) (S.P.); by INSERM, CRE 920113 (M.V.); and by a joint grant CNRS-CONICET (M.V./ S.P.). S.P. is a research member from the CONICET and R.M.B. and K.W. are research fellows from the same institution.
PY - 1995/10
Y1 - 1995/10
N2 - Soluble nucleoside-diphosphate kinase (NDP kinase) from Candida albicans was purified to electrophoretic homogeneity and a partial sequence was determined. The enzyme was kinetically and physically characterized. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified enzyme revealed a single polypeptide of 17 kDa upon staining, by immunodetection with heterologous and homologous antibodies, and by autoradiography of the phosphorylated enzyme. Furthermore, isoelectric focusing of the purified native enzyme showed a single acidic band (pI 4.5). These results together with a native molecular mass of 98 kDa suggest a hexameric native enzyme composed of identical subunits. Like NDP kinases from other sources, the catalysis involves a phosphoenzyme intermediate that is rapidly formed upon incubation of the enzyme with ATP. The transfer of phosphate from phosphoprotein intermediate to nucleoside diphosphates is equally fast. Kinetic experiments indicated that GTP and ATP had the lowest K(m) compared to UTP, dTTP, and CTP. GDP acted as a preferred acceptor as assessed by kinetic measurements as well as by competition experiments. Experimental data are presented indicating the existence of a membrane-associated NDP kinase. Preliminary characterization of this enzyme suggests that cytosolic and membrane-associated NDP kinases are similar proteins.
AB - Soluble nucleoside-diphosphate kinase (NDP kinase) from Candida albicans was purified to electrophoretic homogeneity and a partial sequence was determined. The enzyme was kinetically and physically characterized. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified enzyme revealed a single polypeptide of 17 kDa upon staining, by immunodetection with heterologous and homologous antibodies, and by autoradiography of the phosphorylated enzyme. Furthermore, isoelectric focusing of the purified native enzyme showed a single acidic band (pI 4.5). These results together with a native molecular mass of 98 kDa suggest a hexameric native enzyme composed of identical subunits. Like NDP kinases from other sources, the catalysis involves a phosphoenzyme intermediate that is rapidly formed upon incubation of the enzyme with ATP. The transfer of phosphate from phosphoprotein intermediate to nucleoside diphosphates is equally fast. Kinetic experiments indicated that GTP and ATP had the lowest K(m) compared to UTP, dTTP, and CTP. GDP acted as a preferred acceptor as assessed by kinetic measurements as well as by competition experiments. Experimental data are presented indicating the existence of a membrane-associated NDP kinase. Preliminary characterization of this enzyme suggests that cytosolic and membrane-associated NDP kinases are similar proteins.
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U2 - 10.1006/abbi.1995.0025
DO - 10.1006/abbi.1995.0025
M3 - Article
C2 - 7487065
AN - SCOPUS:0028820544
VL - 323
SP - 187
EP - 194
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
SN - 0003-9861
IS - 1
ER -