Candida albicans Infections: a novel porcine wound model to evaluate treatment efficacy

Joel Gil Rodriguez, Michael Solis, Alexander Higa, Stephen C. Davis

Research output: Contribution to journalArticlepeer-review

Abstract

Candida albicans is a common cause of opportunistic mycoses worldwide and a major contributor in wound infections. The purpose of this study was to establish a fungal wound model and analyze the effects of a common antifungal agent against the proliferation of three C. albicans strains. Second degree burns were created, and then inoculated with one of three different C. albicans ATCC strains: 10261 reference strain, 64550 fluconazole resistant and 26310 fluconazole sensitive. After fungal inoculation, every wound was covered with dressings for 4 h to allow fungal colonization on every wound bed. After 4 h, the dressings were removed, and each wound was treated either once or twice daily with a topical terbinafine hydrochloride or left untreated. On days 2, 4 and 7 post inoculation, three wounds from each treatment group were scrub cultured and quantified. On day 2, wounds infected with the sensitive strains 26310 and 10261 and treated twice showed a significant reduction when compared against those infected wounds receiving once daily treatment. On day 4, wounds which were infected with C. albicans fluconazole sensitive (ATCC 26310) showed a significant reduction in fungal cell counts with treatment applied twice daily. A significant reduction in the colony counts was exhibited in all three strains at the seventh day with active as compared to the non-treated wounds. Twice daily treatment resulted in a lower fungal count than once daily treatment. Neither treatment was able to entirely eradicate C. albicans during the duration of this study. Establishing a reliable fungal wound model will help in the translational goal of identifying new antifungal that could be used clinically by wound care providers.

Original languageEnglish (US)
Article number45
JournalBMC Microbiology
Volume22
Issue number1
DOIs
StatePublished - Dec 2022
Externally publishedYes

Keywords

  • Antifungal
  • Antifungal
  • Biofilm model
  • Burns
  • Candida albicans
  • Infection
  • New model
  • Porcine
  • Porcine model
  • Wound
  • Yeast

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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