TY - JOUR
T1 - cAMP-dependent protein kinase from Mucor rouxii
T2 - Physical evidence of a ternary complex holoenzyme-cAMP
AU - Pastori, R. L.
AU - Kerner, N.
AU - Moreno, S.
AU - Passeron, S.
N1 - Funding Information:
Acknowledgements: This work has been supported by grants from the Consejo National de Investigaciones Cientificas y Tecnicas, (CONICET), Secretaria de Estado de Ciencia y Tecnologia and ComisiBn National de Energia AtBmica. N.K. is a post-graduate Fellow and S.P. and S.M. are Career Investigators of CONICET. The authors wish to express their gratitude to Dr. C.E. Cardini his constant and enthusiastic support.
PY - 1981/7/30
Y1 - 1981/7/30
N2 - Gel electrophoresis and sucrose density gradient centrifugation techniques permitted the visualization for the first time of the ternary complex formed by the binding of cAMP to Mucor rouxii cAMP-dependent protein kinase holoenzyme. The addition of 0.5 M NaCl or histone plus ATP-Mg++, together with cAMP, dissociates the holoenzyme into free regulatory (R) and catalytic (C) subunits. At 4°C, cAMP bound to the holoenzyme is readily exchangeable with unlabeled cAMP (half life 2.5 min), while the nucleotide bound to the R subunit has a very slow exchange rate (half life 210 min). The amount of cAMP bound to R subunit is approximately twice the amount bound to holoenzyme at saturation.
AB - Gel electrophoresis and sucrose density gradient centrifugation techniques permitted the visualization for the first time of the ternary complex formed by the binding of cAMP to Mucor rouxii cAMP-dependent protein kinase holoenzyme. The addition of 0.5 M NaCl or histone plus ATP-Mg++, together with cAMP, dissociates the holoenzyme into free regulatory (R) and catalytic (C) subunits. At 4°C, cAMP bound to the holoenzyme is readily exchangeable with unlabeled cAMP (half life 2.5 min), while the nucleotide bound to the R subunit has a very slow exchange rate (half life 210 min). The amount of cAMP bound to R subunit is approximately twice the amount bound to holoenzyme at saturation.
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U2 - 10.1016/0006-291X(81)91310-3
DO - 10.1016/0006-291X(81)91310-3
M3 - Article
C2 - 6272765
AN - SCOPUS:0019891331
VL - 101
SP - 663
EP - 671
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -