Calvarial Cells Synthesize 1α,25-Dihydroxyvitamin D3 from 25-Hydroxyvitamin D3

Russell T. Turner, Russell T. Turner, Daniel R. Knapp, Guy A. Howard, J. Edward Puzas, David J. Baylink

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


A metabolite of vitamin D has been isolated in pure form from incubation of 25-hydroxyvitamin D3 with embryonic chick calvarial cells that had been grown on Cytodex 1 microcarrier beads. The isolation involved dichloromethane extraction of the cells and incubation medium, followed by Sephadex LH-20 column chromatography and high-performance liquid chromatography of the extract. The metabolite was identified as 1a,25-dihydroxyvitamin D3 by means of ultraviolet absorption spectroscopy, mass spectrometry, and sensitivity to oxidation by periodate. This metabolite was not produced by cell-free medium or by cells from embryonic chick liver, skin, or heart. In conclusion, (1) kidney cells are not unique in having 25-hydroxyvitamin D3: 1α-hydroxylase activity as previously believed and (2) vitamin D target tissues such as the skeleton may play a direct role in mediating the metabolism of 25-hydroxyvitamin D3 to 1α,25-dihydroxyvitamin D3, a vitamin D metabolite active at those sites.

Original languageEnglish (US)
Pages (from-to)1073-1076
Number of pages4
Issue number5
StatePublished - 1983
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry


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