Calvarial cells synthesize 1α,25-dihydroxyvitamin D3 from 25-hydroxyvitamin D3

Russell T. Turner, Guy A. Howard, J. Edward Puzas, Guy Howard, Daniel R. Knapp

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A metabolite of vitamin D has been isolated in pure form from incubation of 25-hydroxyvitamin D3 with embryonic chick calvarial cells that had been grown on Cytodex 1 microcarrier beads. The isolation involved dichloromethane extraction of the cells and incubation medium, followed by Sephadex LH-20 column chromatography and high-performance liquid chromatography of the extract. The metabolite was identified as 1a,25-dihydroxyvitamin D3 by means of ultraviolet absorption spectroscopy, mass spectrometry, and sensitivity to oxidation by periodate. This metabolite was not produced by cell-free medium or by cells from embryonic chick liver, skin, or heart. In conclusion, (1) kidney cells are not unique in having 25-hydroxyvitamin D3: 1α-hydroxylase activity as previously believed and (2) vitamin D target tissues such as the skeleton may play a direct role in mediating the metabolism of 25-hydroxyvitamin D3 to 1α,25-dihydroxyvitamin D3, a vitamin D metabolite active at those sites.

Original languageEnglish
Pages (from-to)1073-1076
Number of pages4
JournalBiochemistry
Volume22
Issue number5
StatePublished - Dec 1 1983
Externally publishedYes

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Calcifediol
Calcitriol
Metabolites
Vitamin D
Ergocalciferols
Column chromatography
Methylene Chloride
High performance liquid chromatography
Mixed Function Oxygenases
Ultraviolet spectroscopy
Absorption spectroscopy
Metabolism
Liver
Mass spectrometry
Skin
Skeleton
Tissue
Chromatography
Mass Spectrometry
Spectrum Analysis

ASJC Scopus subject areas

  • Biochemistry

Cite this

Turner, R. T., Howard, G. A., Edward Puzas, J., Howard, G., & Knapp, D. R. (1983). Calvarial cells synthesize 1α,25-dihydroxyvitamin D3 from 25-hydroxyvitamin D3. Biochemistry, 22(5), 1073-1076.

Calvarial cells synthesize 1α,25-dihydroxyvitamin D3 from 25-hydroxyvitamin D3. / Turner, Russell T.; Howard, Guy A.; Edward Puzas, J.; Howard, Guy; Knapp, Daniel R.

In: Biochemistry, Vol. 22, No. 5, 01.12.1983, p. 1073-1076.

Research output: Contribution to journalArticle

Turner, RT, Howard, GA, Edward Puzas, J, Howard, G & Knapp, DR 1983, 'Calvarial cells synthesize 1α,25-dihydroxyvitamin D3 from 25-hydroxyvitamin D3', Biochemistry, vol. 22, no. 5, pp. 1073-1076.
Turner RT, Howard GA, Edward Puzas J, Howard G, Knapp DR. Calvarial cells synthesize 1α,25-dihydroxyvitamin D3 from 25-hydroxyvitamin D3. Biochemistry. 1983 Dec 1;22(5):1073-1076.
Turner, Russell T. ; Howard, Guy A. ; Edward Puzas, J. ; Howard, Guy ; Knapp, Daniel R. / Calvarial cells synthesize 1α,25-dihydroxyvitamin D3 from 25-hydroxyvitamin D3. In: Biochemistry. 1983 ; Vol. 22, No. 5. pp. 1073-1076.
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