Calibration of micropipets using the bioluminescent protein aequorin

Anne L. Grosvenor; Czarena L. Crofcheck; Kimberly W. Anderson; Donna L. Scott; Sylvia Daunert

doi:10.1021/ac970123a

Calibration of micropipets using the bioluminescent protein aequorin

Anne L. Grosvenor, Czarena L. Crofcheck, Kimberly W. Anderson, Donna L. Scott, Sylvia Daunert

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

A new method for calibrating micropipets and determining accurate injection volumes using a pressure-based injector has been developed. This method employs the bioluminescent protein aequorin and can be used to determine injection volumes as small as 3 pL. The calibration plots are linear over at least 3 orders of magnitude. In contrast to conventional micropipet calibration methods that employ fluorescent molecules, the present method produces small background signals.

Original language	English (US)
Pages (from-to)	3115-3118
Number of pages	4
Journal	Analytical Chemistry
Volume	69
Issue number	15
DOIs	https://doi.org/10.1021/ac970123a
State	Published - Aug 1 1997
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

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abstract = "A new method for calibrating micropipets and determining accurate injection volumes using a pressure-based injector has been developed. This method employs the bioluminescent protein aequorin and can be used to determine injection volumes as small as 3 pL. The calibration plots are linear over at least 3 orders of magnitude. In contrast to conventional micropipet calibration methods that employ fluorescent molecules, the present method produces small background signals.",

author = "Grosvenor, {Anne L.} and Crofcheck, {Czarena L.} and Anderson, {Kimberly W.} and Scott, {Donna L.} and Sylvia Daunert",

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AU - Grosvenor, Anne L.

AU - Crofcheck, Czarena L.

AU - Anderson, Kimberly W.

AU - Scott, Donna L.

AU - Daunert, Sylvia

PY - 1997/8/1

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AB - A new method for calibrating micropipets and determining accurate injection volumes using a pressure-based injector has been developed. This method employs the bioluminescent protein aequorin and can be used to determine injection volumes as small as 3 pL. The calibration plots are linear over at least 3 orders of magnitude. In contrast to conventional micropipet calibration methods that employ fluorescent molecules, the present method produces small background signals.

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