Calibration of micropipets using the bioluminescent protein aequorin

Anne L. Grosvenor, Czarena L. Crofcheck, Kimberly W. Anderson, Donna L. Scott, Sylvia Daunert

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A new method for calibrating micropipets and determining accurate injection volumes using a pressure-based injector has been developed. This method employs the bioluminescent protein aequorin and can be used to determine injection volumes as small as 3 pL. The calibration plots are linear over at least 3 orders of magnitude. In contrast to conventional micropipet calibration methods that employ fluorescent molecules, the present method produces small background signals.

Original languageEnglish
Pages (from-to)3115-3118
Number of pages4
JournalAnalytical Chemistry
Volume69
Issue number15
StatePublished - Aug 1 1997
Externally publishedYes

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Luminescent Proteins
Aequorin
Calibration
Molecules

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Grosvenor, A. L., Crofcheck, C. L., Anderson, K. W., Scott, D. L., & Daunert, S. (1997). Calibration of micropipets using the bioluminescent protein aequorin. Analytical Chemistry, 69(15), 3115-3118.

Calibration of micropipets using the bioluminescent protein aequorin. / Grosvenor, Anne L.; Crofcheck, Czarena L.; Anderson, Kimberly W.; Scott, Donna L.; Daunert, Sylvia.

In: Analytical Chemistry, Vol. 69, No. 15, 01.08.1997, p. 3115-3118.

Research output: Contribution to journalArticle

Grosvenor, AL, Crofcheck, CL, Anderson, KW, Scott, DL & Daunert, S 1997, 'Calibration of micropipets using the bioluminescent protein aequorin', Analytical Chemistry, vol. 69, no. 15, pp. 3115-3118.
Grosvenor AL, Crofcheck CL, Anderson KW, Scott DL, Daunert S. Calibration of micropipets using the bioluminescent protein aequorin. Analytical Chemistry. 1997 Aug 1;69(15):3115-3118.
Grosvenor, Anne L. ; Crofcheck, Czarena L. ; Anderson, Kimberly W. ; Scott, Donna L. ; Daunert, Sylvia. / Calibration of micropipets using the bioluminescent protein aequorin. In: Analytical Chemistry. 1997 ; Vol. 69, No. 15. pp. 3115-3118.
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