Abstract
A new method for calibrating micropipets and determining accurate injection volumes using a pressure-based injector has been developed. This method employs the bioluminescent protein aequorin and can be used to determine injection volumes as small as 3 pL. The calibration plots are linear over at least 3 orders of magnitude. In contrast to conventional micropipet calibration methods that employ fluorescent molecules, the present method produces small background signals.
| Original language | English |
|---|---|
| Pages (from-to) | 3115-3118 |
| Number of pages | 4 |
| Journal | Analytical Chemistry |
| Volume | 69 |
| Issue number | 15 |
| State | Published - Aug 1 1997 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Calibration of micropipets using the bioluminescent protein aequorin. / Grosvenor, Anne L.; Crofcheck, Czarena L.; Anderson, Kimberly W.; Scott, Donna L.; Daunert, Sylvia.
In: Analytical Chemistry, Vol. 69, No. 15, 01.08.1997, p. 3115-3118.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Calibration of micropipets using the bioluminescent protein aequorin
AU - Grosvenor, Anne L.
AU - Crofcheck, Czarena L.
AU - Anderson, Kimberly W.
AU - Scott, Donna L.
AU - Daunert, Sylvia
PY - 1997/8/1
Y1 - 1997/8/1
N2 - A new method for calibrating micropipets and determining accurate injection volumes using a pressure-based injector has been developed. This method employs the bioluminescent protein aequorin and can be used to determine injection volumes as small as 3 pL. The calibration plots are linear over at least 3 orders of magnitude. In contrast to conventional micropipet calibration methods that employ fluorescent molecules, the present method produces small background signals.
AB - A new method for calibrating micropipets and determining accurate injection volumes using a pressure-based injector has been developed. This method employs the bioluminescent protein aequorin and can be used to determine injection volumes as small as 3 pL. The calibration plots are linear over at least 3 orders of magnitude. In contrast to conventional micropipet calibration methods that employ fluorescent molecules, the present method produces small background signals.
UR - http://www.scopus.com/inward/record.url?scp=0031204512&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031204512&partnerID=8YFLogxK
M3 - Article
C2 - 9253255
AN - SCOPUS:0031204512
VL - 69
SP - 3115
EP - 3118
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 15
ER -