Calibration of micropipets using the bioluminescent protein aequorin

Anne L. Grosvenor, Czarena L. Crofcheck, Kimberly W. Anderson, Donna L. Scott, Sylvia Daunert

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

A new method for calibrating micropipets and determining accurate injection volumes using a pressure-based injector has been developed. This method employs the bioluminescent protein aequorin and can be used to determine injection volumes as small as 3 pL. The calibration plots are linear over at least 3 orders of magnitude. In contrast to conventional micropipet calibration methods that employ fluorescent molecules, the present method produces small background signals.

Original languageEnglish (US)
Pages (from-to)3115-3118
Number of pages4
JournalAnalytical Chemistry
Volume69
Issue number15
DOIs
StatePublished - Aug 1 1997
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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