Abstract
A new method for calibrating micropipets and determining accurate injection volumes using a pressure-based injector has been developed. This method employs the bioluminescent protein aequorin and can be used to determine injection volumes as small as 3 pL. The calibration plots are linear over at least 3 orders of magnitude. In contrast to conventional micropipet calibration methods that employ fluorescent molecules, the present method produces small background signals.
Original language | English (US) |
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Pages (from-to) | 3115-3118 |
Number of pages | 4 |
Journal | Analytical Chemistry |
Volume | 69 |
Issue number | 15 |
DOIs | |
State | Published - Aug 1 1997 |
Externally published | Yes |
ASJC Scopus subject areas
- Analytical Chemistry