Calcium transport by isolated skin of rainbow trout.

W. S. Marshall, S. E. Bryson, C. M. Wood

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

The skin overlying the cleithrum bone of freshwater-acclimated rainbow trout contains numerous mitochondria-rich (MR) cells, as detected by DASPEI fluorescence. This tissue was mounted in vitro in an Ussing-style chamber with fresh water on the mucosal surface and saline supplemented with bovine serum albumin on the serosal surface. The preparation developed a high transepithelial resistance and a small transepithelial potential (Vt), positive on the serosal side. Radioisotopic flux measurements indicated that the preparation actively transported Ca2+ from the mucosal to the serosal surface, as assessed by the Ussing flux ratio criterion. Ca2+ transport was positively correlated with MR cell density. Cortisol pretreatment in vivo reduced MR cell density and increased Vt but did not significantly alter Ca2+ fluxes. Ca2+ transport was unaffected by adrenergic agonists (10(-5) mol l-1 adrenaline, clonidine, isoprenaline) or cyclic AMP stimulants (10(-3) mol l-1 dibutyryl cyclic adenosine monophosphate, db-cAMP, plus 10(-4) mol l-1 isobutylmethylxanthine, IBMX) applied to the serosal surface. The Ca2+ ionophore ionomycin (1 x 10(-6)-3.2 x 10(-6) mol l-1 on the mucosal surface) increased both unidirectional Ca2+ fluxes and caused Ca2+ to accumulate within the epithelium. Lanthanum (10(-4) mol l-1) did not inhibit unidirectional Ca2+ fluxes, but apparently displaced Ca2+ from binding sites on the mucosal surface. Unlike Ca2+, movements of Na+ and Cl- across the epithelium were passive, as assessed by the flux ratio criterion, and neither adrenaline nor db-cAMP plus IBMX had any effect on Na+ or Cl- fluxes or electrical properties. These results indicate that ion transport across the skin mediated by MR cells ('chloride cells') contributes to Ca2+ but not to NaCl balance in freshwater trout.

Original languageEnglish
Pages (from-to)297-316
Number of pages20
JournalJournal of Experimental Biology
Volume166
StatePublished - May 1 1992

Fingerprint

Oncorhynchus mykiss
skin (animal)
rainbow
skin
Mitochondria
Fresh Water
calcium
mitochondrion
Calcium
1-Methyl-3-isobutylxanthine
Skin
Cyclic AMP
Epinephrine
Epithelium
Cell Count
Lanthanum
Adrenergic Agonists
Ionomycin
mitochondria
Trout

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Marshall, W. S., Bryson, S. E., & Wood, C. M. (1992). Calcium transport by isolated skin of rainbow trout. Journal of Experimental Biology, 166, 297-316.

Calcium transport by isolated skin of rainbow trout. / Marshall, W. S.; Bryson, S. E.; Wood, C. M.

In: Journal of Experimental Biology, Vol. 166, 01.05.1992, p. 297-316.

Research output: Contribution to journalArticle

Marshall, WS, Bryson, SE & Wood, CM 1992, 'Calcium transport by isolated skin of rainbow trout.', Journal of Experimental Biology, vol. 166, pp. 297-316.
Marshall WS, Bryson SE, Wood CM. Calcium transport by isolated skin of rainbow trout. Journal of Experimental Biology. 1992 May 1;166:297-316.
Marshall, W. S. ; Bryson, S. E. ; Wood, C. M. / Calcium transport by isolated skin of rainbow trout. In: Journal of Experimental Biology. 1992 ; Vol. 166. pp. 297-316.
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