Calcium-sensitive recovery of extracellular potassium and synaptic transmission in rat hippocampal slices exposed to brief anoxia

Eugene L Roberts, Thomas Sick

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

We examined the possibility that Ca2+-sensitive inhibition of synaptic transmission following anoxia involves compromise of ion transport activity. Rat hippocampal slices were superfused with artificial cerebrospinal fluids containing different concentrations of CaCl2, and subjected to short anoxia. Durations of anoxia were sufficient to provoke anoxic depolarization, indicated by a sudden rise in extracellular K+ (K+o). Following anoxia, apparent K+ transport was assessed by measuring the magnitude of subnormal K+o (the K+o undershoot) in hippocampal region CA1. Recovery of synaptic transmission 1 h after anoxia was determined by evaluation of the magnitudes of the orthodromically stimulated population spike recorded from CA1 pyramidal cells. K+o undershoots and recovery of synaptic transmission decreased as CaCl2 or the duration of anoxic depolarization increased. These data suggest: (1) that increased artificial cerebrospinal fluid CaCl2 compromised K+ reaccumulation after anoxia; and (2) that ion transport dysfunction may inhibit recovery of synaptic transmission.

Original languageEnglish
Pages (from-to)113-119
Number of pages7
JournalBrain Research
Volume456
Issue number1
DOIs
StatePublished - Jul 19 1988

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Synaptic Transmission
Potassium
Calcium
Ion Transport
Cerebrospinal Fluid
Hippocampal CA1 Region
Pyramidal Cells
Hypoxia
Population

Keywords

  • Anoxic depolarization
  • Calcium
  • Potassium undershoot
  • Synaptic transmission

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Calcium-sensitive recovery of extracellular potassium and synaptic transmission in rat hippocampal slices exposed to brief anoxia. / Roberts, Eugene L; Sick, Thomas.

In: Brain Research, Vol. 456, No. 1, 19.07.1988, p. 113-119.

Research output: Contribution to journalArticle

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