Calcium-regulatory mechanisms: Functional classification using skinned fibers

W. G.L. Kerrick, P. E. Hoar, P. S. Cassidy, L. Bolles, D. A. Malencik

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


The primary purpose of this study was to determine whether various agents (adenosine 3-thiotriphosphate [ATPγS], trifluoperazine (TFP], troponin I, the catalytic subunit of the cyclic adenosine 3',5'-monophosphate dependent protein kinase [C-subunit], and calmodulin [CaM]) could be used to classify skinned fiber types, and then to determine whether the proposed mechanisms for Ca2+ regulation were consistent with the results. Agents (ATPγS, TFP, C-subunit, CaM) expected to alter a light chain kinase-phosphatase system strongly affect the Ca2+-activated tension in skinned gizzard smooth muscle fibers, whereas these agents have no effect on skinned mammalian striated and scallop adductor fibers. Troponin I, which is known to bind strongly to troponin C and CaM, inhibits Ca2+ activation of skinned mammalian striated and gizzard fibers but not scallop adductor muscle. The results in different types of skinned fibers are consistent with the proposed mechanisms for Ca2+ regulation.

Original languageEnglish (US)
Pages (from-to)177-190
Number of pages14
JournalJournal of General Physiology
Issue number2
StatePublished - Feb 1 1981
Externally publishedYes

ASJC Scopus subject areas

  • Physiology


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