Calcium-regulatory mechanisms. Functional classification using skinned fibers

W. Glenn Kerrick, P. E. Hoar, P. S. Cassidy, L. Bolles, D. A. Malencik

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The primary purpose of this study was to determine whether various agents (adenosine 3-thiotriphosphate [ATPγS], trifluoperazine (TFP], troponin I, the catalytic subunit of the cyclic adenosine 3',5'-monophosphate dependent protein kinase [C-subunit], and calmodulin [CaM]) could be used to classify skinned fiber types, and then to determine whether the proposed mechanisms for Ca2+ regulation were consistent with the results. Agents (ATPγS, TFP, C-subunit, CaM) expected to alter a light chain kinase-phosphatase system strongly affect the Ca2+-activated tension in skinned gizzard smooth muscle fibers, whereas these agents have no effect on skinned mammalian striated and scallop adductor fibers. Troponin I, which is known to bind strongly to troponin C and CaM, inhibits Ca2+ activation of skinned mammalian striated and gizzard fibers but not scallop adductor muscle. The results in different types of skinned fibers are consistent with the proposed mechanisms for Ca2+ regulation.

Original languageEnglish
Pages (from-to)177-190
Number of pages14
JournalJournal of General Physiology
Volume77
Issue number2
StatePublished - Dec 1 1981
Externally publishedYes

Fingerprint

Pectinidae
Troponin I
Calmodulin
Adenosine
Troponin C
Calcium
Trifluoperazine
Calcium-Calmodulin-Dependent Protein Kinases
Phosphoric Monoester Hydrolases
Cyclic AMP
Protein Kinase C
Smooth Muscle
Catalytic Domain
Phosphotransferases
Light
Muscles

ASJC Scopus subject areas

  • Physiology

Cite this

Kerrick, W. G., Hoar, P. E., Cassidy, P. S., Bolles, L., & Malencik, D. A. (1981). Calcium-regulatory mechanisms. Functional classification using skinned fibers. Journal of General Physiology, 77(2), 177-190.

Calcium-regulatory mechanisms. Functional classification using skinned fibers. / Kerrick, W. Glenn; Hoar, P. E.; Cassidy, P. S.; Bolles, L.; Malencik, D. A.

In: Journal of General Physiology, Vol. 77, No. 2, 01.12.1981, p. 177-190.

Research output: Contribution to journalArticle

Kerrick, WG, Hoar, PE, Cassidy, PS, Bolles, L & Malencik, DA 1981, 'Calcium-regulatory mechanisms. Functional classification using skinned fibers', Journal of General Physiology, vol. 77, no. 2, pp. 177-190.
Kerrick, W. Glenn ; Hoar, P. E. ; Cassidy, P. S. ; Bolles, L. ; Malencik, D. A. / Calcium-regulatory mechanisms. Functional classification using skinned fibers. In: Journal of General Physiology. 1981 ; Vol. 77, No. 2. pp. 177-190.
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