Calcium-regulated type III secretion of Yop proteins by an Escherichia coli hha mutant carrying a Yersinia pestis pCD1 virulence plasmid

Sara Schesser Bartra, Michael W. Jackson, Julia A. Ross, Gregory V. Plano

Research output: Contribution to journalArticle

17 Scopus citations


A series of four large deletions that removed a total of ca. 36 kb of DNA from the ca. 70-kb Yersinia pestis pCD1 virulence plasmid were constructed using lambda Red-mediated recombination. Escherichia coli hha deletion mutants carrying the virulence plasmid with the deletions expressed a functional calcium-regulated type III secretion system. The E. coli hha/pCD1 system should facilitate molecular studies of the type III secretion process.

Original languageEnglish (US)
Pages (from-to)1381-1386
Number of pages6
JournalInfection and Immunity
Issue number2
StatePublished - Feb 1 2006


ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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