Calcium phosphate particle induction of metalloproteinase and mitogenesis: Effect of particle sizes

Herman S Cheung, T. R. Devine, W. Hubbard

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Calcium pyrophosphate dihydrate (CPPD) and basic calcium phosphate (BCP) crystals [hydroxyapatite (HA), octacalcium phosphate, tricalcium phosphate] are common in osteoarthritis knee effusions, and are often associated with low-grade synovial proliferation and inflammation. Calcium-containing crystals including HA, are known to have a number of biologic effects on culture cells such induction of mitogenesis, stimulation of Prostaglandin E2 (PGE3) production via the phospholipase A2/cyclo-oxygenase pathway, activation of phospholipase C and inositol phospholipid hydrolysis, induction of metalloproteinase synthesis and induction of proto-oncogenes (c-fos and c- myc). While endocytosis of HA particles is prerequisite of the mitogenic effect of calcium-containing crystals in fibroblasts, it is not known whether endocytosis is required for crystal-induced metalloproteinase synthesis. In the present series of experiments, we examine the effect of three different sizes (106, 46, and 17 μm mean diameters) well-characterized spherical HA particles on the induction of mitogenesis and metalloproteinase synthesis on human fibroblasts. We showed that endocytosis is required for HA particles to induce synthesis of metalloproteinases.

Original languageEnglish
Pages (from-to)145-151
Number of pages7
JournalOsteoarthritis and Cartilage
Volume5
Issue number3
DOIs
StatePublished - Jul 3 1997
Externally publishedYes

Fingerprint

Calcium phosphate
Metalloproteases
Durapatite
Hydroxyapatite
Particle Size
Particle size
Endocytosis
Calcium
Crystals
Fibroblasts
Phosphates
Calcium Pyrophosphate
fos Genes
Knee Osteoarthritis
Phospholipases A2
Phospholipids
Type C Phospholipases
Prostaglandin-Endoperoxide Synthases
Phosphatidylinositols
Cell culture

Keywords

  • Endocytosis
  • Hydroxyapatite
  • Metalloproteinase
  • Particle size

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Calcium phosphate particle induction of metalloproteinase and mitogenesis : Effect of particle sizes. / Cheung, Herman S; Devine, T. R.; Hubbard, W.

In: Osteoarthritis and Cartilage, Vol. 5, No. 3, 03.07.1997, p. 145-151.

Research output: Contribution to journalArticle

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