Calcium-mediated, purinergic stimulation and polarized localization of calcium-sensitive adenylyl cyclase isoforms in human airway epithelia

Marie Christine Nlend, Andreas Schmid, Zoltan Sutto, George A. Ransford, Gregory E Conner, Nevis L. Fregien, Matthias A Salathe

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Purinergic stimulation of human airway epithelia results in a prolonged increase in ciliary beat frequency that depends on calcium-mediated cAMP production [Lieb, T., Wijkstrom Frei, C., Frohock, J.I., Bookman, R.J. and Salathe, M. (2002) Prolonged increase in ciliary beat frequency after short-term purinergic stimulation in human airway epithelial cells. J. Physiol. (Lond.) 538, 633-646]. Here, fully differentiated human airway epithelial cells in culture are shown to express calcium-stimulated transmembrane adenylyl cyclase (tmAC) isoforms (types 1, 3, and 8) by reverse transcription polymerase chain reaction. Immunohistochemistry of tracheal sections and fully differentiated airway epithelial cell cultures revealed polarized expression of these tmACs, with types 1 and 8 localized to the apical membrane and thus at the position required for ciliary regulation. Real-time, ciliated-cell specific cAMP production by tmACs upon apical, purinergic stimulation with UTP was confirmed using fluorescent energy resonance transfer between fluorescently tagged PKA subunits.

Original languageEnglish
Pages (from-to)3241-3246
Number of pages6
JournalFEBS Letters
Volume581
Issue number17
DOIs
StatePublished - Jul 10 2007

Fingerprint

Adenylyl Cyclases
Protein Isoforms
Epithelium
Epithelial Cells
Calcium
Cell Culture Techniques
Uridine Triphosphate
Energy Transfer
Reverse Transcription
Polymerase chain reaction
Transcription
Immunohistochemistry
Cell culture
Polymerase Chain Reaction
Membranes

Keywords

  • Adenylyl cyclase isoforms
  • Airway epithelium
  • cAMP production
  • Ciliary beating
  • Polarized expression

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Calcium-mediated, purinergic stimulation and polarized localization of calcium-sensitive adenylyl cyclase isoforms in human airway epithelia. / Nlend, Marie Christine; Schmid, Andreas; Sutto, Zoltan; Ransford, George A.; Conner, Gregory E; Fregien, Nevis L.; Salathe, Matthias A.

In: FEBS Letters, Vol. 581, No. 17, 10.07.2007, p. 3241-3246.

Research output: Contribution to journalArticle

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