Calcium induced fusion of plasma membranes isolated from myoblasts grown in culture

C. Schudt; G. Dahl; M. Gratzl

Calcium induced fusion of plasma membranes isolated from myoblasts grown in culture

C. Schudt, G. Dahl, M. Gratzl

Research output: Contribution to journal › Article › peer-review

Abstract

Plasma membrane vesicles isolated from myoblasts grown in vitro were found to fuse in the presence of calcium ions. As revealed by freeze cleaving techniques, addition of 1.4mM CaCl₂ or 1.4mM MgCl₂ resulted in attachment of vesicles and aggregation of intramembranous particles. Plasma membrane vesicles fuse solely in the presence of calcium, and fusion is identified by the appearance of twinned vesicles. Pore formation was preceded by an aggregation of intramembranous particles in the area of membrane contact. 20mM MgCl₂ or 10^-6M lysolecithin both decreased the amount of vesicle fusion in a similar manner to the inhibition of the fusion of myoblasts.

Original language	English (US)
Pages (from-to)	211-223
Number of pages	13
Journal	Cytobiologie
Volume	13
Issue number	2
State	Published - Jan 1 1976
Externally published	Yes

ASJC Scopus subject areas

Anatomy
Cell Biology

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abstract = "Plasma membrane vesicles isolated from myoblasts grown in vitro were found to fuse in the presence of calcium ions. As revealed by freeze cleaving techniques, addition of 1.4mM CaCl2 or 1.4mM MgCl2 resulted in attachment of vesicles and aggregation of intramembranous particles. Plasma membrane vesicles fuse solely in the presence of calcium, and fusion is identified by the appearance of twinned vesicles. Pore formation was preceded by an aggregation of intramembranous particles in the area of membrane contact. 20mM MgCl2 or 10-6M lysolecithin both decreased the amount of vesicle fusion in a similar manner to the inhibition of the fusion of myoblasts.",

author = "C. Schudt and G. Dahl and M. Gratzl",

year = "1976",

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language = "English (US)",

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AU - Dahl, G.

AU - Gratzl, M.

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N2 - Plasma membrane vesicles isolated from myoblasts grown in vitro were found to fuse in the presence of calcium ions. As revealed by freeze cleaving techniques, addition of 1.4mM CaCl2 or 1.4mM MgCl2 resulted in attachment of vesicles and aggregation of intramembranous particles. Plasma membrane vesicles fuse solely in the presence of calcium, and fusion is identified by the appearance of twinned vesicles. Pore formation was preceded by an aggregation of intramembranous particles in the area of membrane contact. 20mM MgCl2 or 10-6M lysolecithin both decreased the amount of vesicle fusion in a similar manner to the inhibition of the fusion of myoblasts.

AB - Plasma membrane vesicles isolated from myoblasts grown in vitro were found to fuse in the presence of calcium ions. As revealed by freeze cleaving techniques, addition of 1.4mM CaCl2 or 1.4mM MgCl2 resulted in attachment of vesicles and aggregation of intramembranous particles. Plasma membrane vesicles fuse solely in the presence of calcium, and fusion is identified by the appearance of twinned vesicles. Pore formation was preceded by an aggregation of intramembranous particles in the area of membrane contact. 20mM MgCl2 or 10-6M lysolecithin both decreased the amount of vesicle fusion in a similar manner to the inhibition of the fusion of myoblasts.

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