Plasma membrane vesicles isolated from myoblasts grown in vitro were found to fuse in the presence of calcium ions. As revealed by freeze cleaving techniques, addition of 1.4mM CaCl2 or 1.4mM MgCl2 resulted in attachment of vesicles and aggregation of intramembranous particles. Plasma membrane vesicles fuse solely in the presence of calcium, and fusion is identified by the appearance of twinned vesicles. Pore formation was preceded by an aggregation of intramembranous particles in the area of membrane contact. 20mM MgCl2 or 10-6M lysolecithin both decreased the amount of vesicle fusion in a similar manner to the inhibition of the fusion of myoblasts.
|Original language||English (US)|
|Number of pages||13|
|State||Published - Jan 1 1976|
ASJC Scopus subject areas
- Cell Biology