Calcium-dependent protein kinase-C activity in human adrenocortical neoplasms, hyperplastic adrenals, and normal adrenocortical tissue

A. C. Latronico, B. B. Mendonça, A. C. Bianco, S. M. Villares, M. A. Lucon, W. Nicolau, B. L. Wajchenberg

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The calcium- and phospholipid-dependent protein kinase-C (PKC) is a critical enzyme of cellular signal transduction. In this report we studied calcium-dependent total PKC activity in eight adrenocortical carcinomas (group 1), nine adrenocortical adenomas (group 2), six hyperplasias (group 3), and five human normal adrenal tissues (group 4). The PKC activity assay was based on phosphorylation of a specific synthetic peptide from myelin basic protein. The specificity of the assay was confirmed by using an inhibitor peptide common to α-, β-, and γ-isoenzymes of PKC. The median value in group 1 was 1.15 pmol 32P/min · μg protein (range, 0.55-2.19), that in group 2 was 1.2 (range, 0.74-2.7), that in group 3 was 0.915 (range, 0.6-1.7), and that in group 4 was 1.22 (range, 0.6-3.95). The calcium- dependent total PKC activity was similar in the four groups studied. We did not find any correlation between urinary total cortisol, serum cortisol, testosterone, dehydroepiandrosterone, dehydroepiandrosterone sulfate, androstenedione, aldosterone, and estradiol concentrations and PKC activity. These findings suggest that the calcium-dependent PKC activity is not elevated in adrenocortical tumors and is not a useful marker of adrenocortical malignancy.

Original languageEnglish
Pages (from-to)736-739
Number of pages4
JournalJournal of Clinical Endocrinology and Metabolism
Volume79
Issue number3
DOIs
StatePublished - Sep 1 1994

Fingerprint

Adrenal Gland Neoplasms
Human Activities
Protein Kinase C
Tissue
Hydrocortisone
Assays
Adrenocortical Adenoma
Adrenocortical Carcinoma
Calcium
Signal transduction
Dehydroepiandrosterone Sulfate
Peptides
Phosphorylation
Myelin Basic Protein
Dehydroepiandrosterone
calcium-dependent protein kinase
Androstenedione
Aldosterone
Isoenzymes
Hyperplasia

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Calcium-dependent protein kinase-C activity in human adrenocortical neoplasms, hyperplastic adrenals, and normal adrenocortical tissue. / Latronico, A. C.; Mendonça, B. B.; Bianco, A. C.; Villares, S. M.; Lucon, M. A.; Nicolau, W.; Wajchenberg, B. L.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 79, No. 3, 01.09.1994, p. 736-739.

Research output: Contribution to journalArticle

Latronico, A. C. ; Mendonça, B. B. ; Bianco, A. C. ; Villares, S. M. ; Lucon, M. A. ; Nicolau, W. ; Wajchenberg, B. L. / Calcium-dependent protein kinase-C activity in human adrenocortical neoplasms, hyperplastic adrenals, and normal adrenocortical tissue. In: Journal of Clinical Endocrinology and Metabolism. 1994 ; Vol. 79, No. 3. pp. 736-739.
@article{b83d01f2fa904a5284fc08fe048d7b60,
title = "Calcium-dependent protein kinase-C activity in human adrenocortical neoplasms, hyperplastic adrenals, and normal adrenocortical tissue",
abstract = "The calcium- and phospholipid-dependent protein kinase-C (PKC) is a critical enzyme of cellular signal transduction. In this report we studied calcium-dependent total PKC activity in eight adrenocortical carcinomas (group 1), nine adrenocortical adenomas (group 2), six hyperplasias (group 3), and five human normal adrenal tissues (group 4). The PKC activity assay was based on phosphorylation of a specific synthetic peptide from myelin basic protein. The specificity of the assay was confirmed by using an inhibitor peptide common to α-, β-, and γ-isoenzymes of PKC. The median value in group 1 was 1.15 pmol 32P/min · μg protein (range, 0.55-2.19), that in group 2 was 1.2 (range, 0.74-2.7), that in group 3 was 0.915 (range, 0.6-1.7), and that in group 4 was 1.22 (range, 0.6-3.95). The calcium- dependent total PKC activity was similar in the four groups studied. We did not find any correlation between urinary total cortisol, serum cortisol, testosterone, dehydroepiandrosterone, dehydroepiandrosterone sulfate, androstenedione, aldosterone, and estradiol concentrations and PKC activity. These findings suggest that the calcium-dependent PKC activity is not elevated in adrenocortical tumors and is not a useful marker of adrenocortical malignancy.",
author = "Latronico, {A. C.} and Mendon{\cc}a, {B. B.} and Bianco, {A. C.} and Villares, {S. M.} and Lucon, {M. A.} and W. Nicolau and Wajchenberg, {B. L.}",
year = "1994",
month = "9",
day = "1",
doi = "10.1210/jc.79.3.736",
language = "English",
volume = "79",
pages = "736--739",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0021-972X",
publisher = "The Endocrine Society",
number = "3",

}

TY - JOUR

T1 - Calcium-dependent protein kinase-C activity in human adrenocortical neoplasms, hyperplastic adrenals, and normal adrenocortical tissue

AU - Latronico, A. C.

AU - Mendonça, B. B.

AU - Bianco, A. C.

AU - Villares, S. M.

AU - Lucon, M. A.

AU - Nicolau, W.

AU - Wajchenberg, B. L.

PY - 1994/9/1

Y1 - 1994/9/1

N2 - The calcium- and phospholipid-dependent protein kinase-C (PKC) is a critical enzyme of cellular signal transduction. In this report we studied calcium-dependent total PKC activity in eight adrenocortical carcinomas (group 1), nine adrenocortical adenomas (group 2), six hyperplasias (group 3), and five human normal adrenal tissues (group 4). The PKC activity assay was based on phosphorylation of a specific synthetic peptide from myelin basic protein. The specificity of the assay was confirmed by using an inhibitor peptide common to α-, β-, and γ-isoenzymes of PKC. The median value in group 1 was 1.15 pmol 32P/min · μg protein (range, 0.55-2.19), that in group 2 was 1.2 (range, 0.74-2.7), that in group 3 was 0.915 (range, 0.6-1.7), and that in group 4 was 1.22 (range, 0.6-3.95). The calcium- dependent total PKC activity was similar in the four groups studied. We did not find any correlation between urinary total cortisol, serum cortisol, testosterone, dehydroepiandrosterone, dehydroepiandrosterone sulfate, androstenedione, aldosterone, and estradiol concentrations and PKC activity. These findings suggest that the calcium-dependent PKC activity is not elevated in adrenocortical tumors and is not a useful marker of adrenocortical malignancy.

AB - The calcium- and phospholipid-dependent protein kinase-C (PKC) is a critical enzyme of cellular signal transduction. In this report we studied calcium-dependent total PKC activity in eight adrenocortical carcinomas (group 1), nine adrenocortical adenomas (group 2), six hyperplasias (group 3), and five human normal adrenal tissues (group 4). The PKC activity assay was based on phosphorylation of a specific synthetic peptide from myelin basic protein. The specificity of the assay was confirmed by using an inhibitor peptide common to α-, β-, and γ-isoenzymes of PKC. The median value in group 1 was 1.15 pmol 32P/min · μg protein (range, 0.55-2.19), that in group 2 was 1.2 (range, 0.74-2.7), that in group 3 was 0.915 (range, 0.6-1.7), and that in group 4 was 1.22 (range, 0.6-3.95). The calcium- dependent total PKC activity was similar in the four groups studied. We did not find any correlation between urinary total cortisol, serum cortisol, testosterone, dehydroepiandrosterone, dehydroepiandrosterone sulfate, androstenedione, aldosterone, and estradiol concentrations and PKC activity. These findings suggest that the calcium-dependent PKC activity is not elevated in adrenocortical tumors and is not a useful marker of adrenocortical malignancy.

UR - http://www.scopus.com/inward/record.url?scp=0027936762&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027936762&partnerID=8YFLogxK

U2 - 10.1210/jc.79.3.736

DO - 10.1210/jc.79.3.736

M3 - Article

C2 - 7521351

AN - SCOPUS:0027936762

VL - 79

SP - 736

EP - 739

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0021-972X

IS - 3

ER -