Calcium and pancreatic β-cell function. XI. Modification of 45Ca fluxes by Na+ removal

B. Hellman, T. Andersson, P. O. Berggren, P. Rorsman

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Pancreatic islets were isolated from noninbred ob/ob mice and used for evaluating how removal of extracellular Na+ affects 45Ca fluxes in the insulin-producing β cells. Isoosmotic replacement of Na+ with Li+ was less efficient in stimulating the incorporation of intracellular (La3+ nondisplaceable) 45Ca than substitution with choline+ or sucrose. Ample support for the significance of Na+-Ca2+ exchange for the net efflux of Ca2+ was obtained with the observation that Na+ deprivation markedly inhibited the 45Ca washout in a Ca2+-deficient medium. The inhibitory action of Na+ deficiency exceeded that obtained with 20 mM glucose. The 45Ca incorporated after removal of Na+ differed from that taken in response to glucose in not being exchanged with the Ca2+ entering the β cells during glucose stimulation and in appearing also in the postmicrosomal supernatant obtained by differential centrifugation.

Original languageEnglish
Pages (from-to)143-152
Number of pages10
JournalBiochemical Medicine
Volume24
Issue number2
StatePublished - Dec 1 1980
Externally publishedYes

Fingerprint

Fluxes
Calcium
Glucose
Centrifugation
Choline
Islets of Langerhans
Sucrose
Substitution reactions
Insulin

ASJC Scopus subject areas

  • Biochemistry

Cite this

Hellman, B., Andersson, T., Berggren, P. O., & Rorsman, P. (1980). Calcium and pancreatic β-cell function. XI. Modification of 45Ca fluxes by Na+ removal. Biochemical Medicine, 24(2), 143-152.

Calcium and pancreatic β-cell function. XI. Modification of 45Ca fluxes by Na+ removal. / Hellman, B.; Andersson, T.; Berggren, P. O.; Rorsman, P.

In: Biochemical Medicine, Vol. 24, No. 2, 01.12.1980, p. 143-152.

Research output: Contribution to journalArticle

Hellman, B, Andersson, T, Berggren, PO & Rorsman, P 1980, 'Calcium and pancreatic β-cell function. XI. Modification of 45Ca fluxes by Na+ removal', Biochemical Medicine, vol. 24, no. 2, pp. 143-152.
Hellman B, Andersson T, Berggren PO, Rorsman P. Calcium and pancreatic β-cell function. XI. Modification of 45Ca fluxes by Na+ removal. Biochemical Medicine. 1980 Dec 1;24(2):143-152.
Hellman, B. ; Andersson, T. ; Berggren, P. O. ; Rorsman, P. / Calcium and pancreatic β-cell function. XI. Modification of 45Ca fluxes by Na+ removal. In: Biochemical Medicine. 1980 ; Vol. 24, No. 2. pp. 143-152.
@article{dfb5a68cd7d6400fbf0568c7fc9e8faa,
title = "Calcium and pancreatic β-cell function. XI. Modification of 45Ca fluxes by Na+ removal",
abstract = "Pancreatic islets were isolated from noninbred ob/ob mice and used for evaluating how removal of extracellular Na+ affects 45Ca fluxes in the insulin-producing β cells. Isoosmotic replacement of Na+ with Li+ was less efficient in stimulating the incorporation of intracellular (La3+ nondisplaceable) 45Ca than substitution with choline+ or sucrose. Ample support for the significance of Na+-Ca2+ exchange for the net efflux of Ca2+ was obtained with the observation that Na+ deprivation markedly inhibited the 45Ca washout in a Ca2+-deficient medium. The inhibitory action of Na+ deficiency exceeded that obtained with 20 mM glucose. The 45Ca incorporated after removal of Na+ differed from that taken in response to glucose in not being exchanged with the Ca2+ entering the β cells during glucose stimulation and in appearing also in the postmicrosomal supernatant obtained by differential centrifugation.",
author = "B. Hellman and T. Andersson and Berggren, {P. O.} and P. Rorsman",
year = "1980",
month = "12",
day = "1",
language = "English",
volume = "24",
pages = "143--152",
journal = "Molecular Genetics and Metabolism",
issn = "1096-7192",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Calcium and pancreatic β-cell function. XI. Modification of 45Ca fluxes by Na+ removal

AU - Hellman, B.

AU - Andersson, T.

AU - Berggren, P. O.

AU - Rorsman, P.

PY - 1980/12/1

Y1 - 1980/12/1

N2 - Pancreatic islets were isolated from noninbred ob/ob mice and used for evaluating how removal of extracellular Na+ affects 45Ca fluxes in the insulin-producing β cells. Isoosmotic replacement of Na+ with Li+ was less efficient in stimulating the incorporation of intracellular (La3+ nondisplaceable) 45Ca than substitution with choline+ or sucrose. Ample support for the significance of Na+-Ca2+ exchange for the net efflux of Ca2+ was obtained with the observation that Na+ deprivation markedly inhibited the 45Ca washout in a Ca2+-deficient medium. The inhibitory action of Na+ deficiency exceeded that obtained with 20 mM glucose. The 45Ca incorporated after removal of Na+ differed from that taken in response to glucose in not being exchanged with the Ca2+ entering the β cells during glucose stimulation and in appearing also in the postmicrosomal supernatant obtained by differential centrifugation.

AB - Pancreatic islets were isolated from noninbred ob/ob mice and used for evaluating how removal of extracellular Na+ affects 45Ca fluxes in the insulin-producing β cells. Isoosmotic replacement of Na+ with Li+ was less efficient in stimulating the incorporation of intracellular (La3+ nondisplaceable) 45Ca than substitution with choline+ or sucrose. Ample support for the significance of Na+-Ca2+ exchange for the net efflux of Ca2+ was obtained with the observation that Na+ deprivation markedly inhibited the 45Ca washout in a Ca2+-deficient medium. The inhibitory action of Na+ deficiency exceeded that obtained with 20 mM glucose. The 45Ca incorporated after removal of Na+ differed from that taken in response to glucose in not being exchanged with the Ca2+ entering the β cells during glucose stimulation and in appearing also in the postmicrosomal supernatant obtained by differential centrifugation.

UR - http://www.scopus.com/inward/record.url?scp=0019126246&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019126246&partnerID=8YFLogxK

M3 - Article

C2 - 7006599

AN - SCOPUS:0019126246

VL - 24

SP - 143

EP - 152

JO - Molecular Genetics and Metabolism

JF - Molecular Genetics and Metabolism

SN - 1096-7192

IS - 2

ER -