Calcitonin secretion in vitro. I. preparation of monolayer c-cell cultures

B. A. Roos, L. L. Bundy, R. Bailey, L. J. Deftos

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


Monolayer cell cultures of calcitonin secreting cells have been derived from trout ultimobranchial glands. These cultures have been used to study calcitonin secretion and its regulation in vitro; calcitonin has been measured by radioimmunoassay. The cultures exhibit linear hormonal secretion rates and secretory responses to several substances at concentrations as low as 10-8M. Pancreozymin, prostaglandins and dibutyryl cAMP cause at least a 5 fold increase in calcitonin secretion; calcium has a small secretory effect. This system affords a unique method for the study of both the extracellular and intracellular factors involved in the regulation of calcitonin secretion.

Original languageEnglish (US)
Pages (from-to)1142-1149
Number of pages8
Issue number4
StatePublished - Jan 1 1974

ASJC Scopus subject areas

  • Endocrinology


Dive into the research topics of 'Calcitonin secretion in vitro. I. preparation of monolayer c-cell cultures'. Together they form a unique fingerprint.

Cite this