Abstract
Monolayer cell cultures of calcitonin secreting cells have been derived from trout ultimobranchial glands. These cultures have been used to study calcitonin secretion and its regulation in vitro; calcitonin has been measured by radioimmunoassay. The cultures exhibit linear hormonal secretion rates and secretory responses to several substances at concentrations as low as 10-8M. Pancreozymin, prostaglandins and dibutyryl cAMP cause at least a 5 fold increase in calcitonin secretion; calcium has a small secretory effect. This system affords a unique method for the study of both the extracellular and intracellular factors involved in the regulation of calcitonin secretion.
| Original language | English |
|---|---|
| Pages (from-to) | 1142-1149 |
| Number of pages | 8 |
| Journal | Endocrinology |
| Volume | 95 |
| Issue number | 4 |
| State | Published - Dec 1 1974 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Endocrinology
- Endocrinology, Diabetes and Metabolism
Cite this
Calcitonin secretion in vitro. I. Preparation of monolayer C cell cultures. / Roos, B. A.; Bundy, L. L.; Bailey, R.; Deftos, L. J.
In: Endocrinology, Vol. 95, No. 4, 01.12.1974, p. 1142-1149.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Calcitonin secretion in vitro. I. Preparation of monolayer C cell cultures
AU - Roos, B. A.
AU - Bundy, L. L.
AU - Bailey, R.
AU - Deftos, L. J.
PY - 1974/12/1
Y1 - 1974/12/1
N2 - Monolayer cell cultures of calcitonin secreting cells have been derived from trout ultimobranchial glands. These cultures have been used to study calcitonin secretion and its regulation in vitro; calcitonin has been measured by radioimmunoassay. The cultures exhibit linear hormonal secretion rates and secretory responses to several substances at concentrations as low as 10-8M. Pancreozymin, prostaglandins and dibutyryl cAMP cause at least a 5 fold increase in calcitonin secretion; calcium has a small secretory effect. This system affords a unique method for the study of both the extracellular and intracellular factors involved in the regulation of calcitonin secretion.
AB - Monolayer cell cultures of calcitonin secreting cells have been derived from trout ultimobranchial glands. These cultures have been used to study calcitonin secretion and its regulation in vitro; calcitonin has been measured by radioimmunoassay. The cultures exhibit linear hormonal secretion rates and secretory responses to several substances at concentrations as low as 10-8M. Pancreozymin, prostaglandins and dibutyryl cAMP cause at least a 5 fold increase in calcitonin secretion; calcium has a small secretory effect. This system affords a unique method for the study of both the extracellular and intracellular factors involved in the regulation of calcitonin secretion.
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UR - http://www.scopus.com/inward/citedby.url?scp=0016293980&partnerID=8YFLogxK
M3 - Article
C2 - 4369822
AN - SCOPUS:0016293980
VL - 95
SP - 1142
EP - 1149
JO - Endocrinology
JF - Endocrinology
SN - 0013-7227
IS - 4
ER -