Calcitonin secretion in vitro. I. preparation of monolayer c-cell cultures

B. A. Roos, L. L. Bundy, R. Bailey, L. J. Deftos

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Abstract

Monolayer cell cultures of calcitonin secreting cells have been derived from trout ultimobranchial glands. These cultures have been used to study calcitonin secretion and its regulation in vitro; calcitonin has been measured by radioimmunoassay. The cultures exhibit linear hormonal secretion rates and secretory responses to several substances at concentrations as low as 10-8M. Pancreozymin, prostaglandins and dibutyryl cAMP cause at least a 5 fold increase in calcitonin secretion; calcium has a small secretory effect. This system affords a unique method for the study of both the extracellular and intracellular factors involved in the regulation of calcitonin secretion.

Original languageEnglish (US)
Pages (from-to)1142-1149
Number of pages8
JournalEndocrinology
Volume95
Issue number4
DOIs
StatePublished - Jan 1 1974

ASJC Scopus subject areas

  • Endocrinology

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    Roos, B. A., Bundy, L. L., Bailey, R., & Deftos, L. J. (1974). Calcitonin secretion in vitro. I. preparation of monolayer c-cell cultures. Endocrinology, 95(4), 1142-1149. https://doi.org/10.1210/endo-95-4-1142