Monolayer cell cultures of calcitonin secreting cells have been derived from trout ultimobranchial glands. These cultures have been used to study calcitonin secretion and its regulation in vitro; calcitonin has been measured by radioimmunoassay. The cultures exhibit linear hormonal secretion rates and secretory responses to several substances at concentrations as low as 10-8M. Pancreozymin, prostaglandins and dibutyryl cAMP cause at least a 5 fold increase in calcitonin secretion; calcium has a small secretory effect. This system affords a unique method for the study of both the extracellular and intracellular factors involved in the regulation of calcitonin secretion.
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