Calcitonin secretion in vitro. I. preparation of monolayer c-cell cultures

B. A. Roos; L. L. Bundy; R. Bailey; L. J. Deftos

doi:10.1210/endo-95-4-1142

Calcitonin secretion in vitro. I. preparation of monolayer c-cell cultures

B. A. Roos, L. L. Bundy, R. Bailey, L. J. Deftos

Medicine

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Monolayer cell cultures of calcitonin secreting cells have been derived from trout ultimobranchial glands. These cultures have been used to study calcitonin secretion and its regulation in vitro; calcitonin has been measured by radioimmunoassay. The cultures exhibit linear hormonal secretion rates and secretory responses to several substances at concentrations as low as 10^-8M. Pancreozymin, prostaglandins and dibutyryl cAMP cause at least a 5 fold increase in calcitonin secretion; calcium has a small secretory effect. This system affords a unique method for the study of both the extracellular and intracellular factors involved in the regulation of calcitonin secretion.

Original language	English (US)
Pages (from-to)	1142-1149
Number of pages	8
Journal	Endocrinology
Volume	95
Issue number	4
DOIs	https://doi.org/10.1210/endo-95-4-1142
State	Published - Jan 1 1974

ASJC Scopus subject areas

Endocrinology

Access to Document

10.1210/endo-95-4-1142

Cite this

@article{f89259606bd946be8e9f9c43037740c9,

title = "Calcitonin secretion in vitro. I. preparation of monolayer c-cell cultures",

abstract = "Monolayer cell cultures of calcitonin secreting cells have been derived from trout ultimobranchial glands. These cultures have been used to study calcitonin secretion and its regulation in vitro; calcitonin has been measured by radioimmunoassay. The cultures exhibit linear hormonal secretion rates and secretory responses to several substances at concentrations as low as 10-8M. Pancreozymin, prostaglandins and dibutyryl cAMP cause at least a 5 fold increase in calcitonin secretion; calcium has a small secretory effect. This system affords a unique method for the study of both the extracellular and intracellular factors involved in the regulation of calcitonin secretion.",

author = "Roos, {B. A.} and Bundy, {L. L.} and R. Bailey and Deftos, {L. J.}",

year = "1974",

month = jan,

day = "1",

doi = "10.1210/endo-95-4-1142",

language = "English (US)",

volume = "95",

pages = "1142--1149",

journal = "Endocrinology",

issn = "0013-7227",

publisher = "The Endocrine Society",

number = "4",

}

TY - JOUR

T1 - Calcitonin secretion in vitro. I. preparation of monolayer c-cell cultures

AU - Roos, B. A.

AU - Bundy, L. L.

AU - Bailey, R.

AU - Deftos, L. J.

PY - 1974/1/1

Y1 - 1974/1/1

N2 - Monolayer cell cultures of calcitonin secreting cells have been derived from trout ultimobranchial glands. These cultures have been used to study calcitonin secretion and its regulation in vitro; calcitonin has been measured by radioimmunoassay. The cultures exhibit linear hormonal secretion rates and secretory responses to several substances at concentrations as low as 10-8M. Pancreozymin, prostaglandins and dibutyryl cAMP cause at least a 5 fold increase in calcitonin secretion; calcium has a small secretory effect. This system affords a unique method for the study of both the extracellular and intracellular factors involved in the regulation of calcitonin secretion.

AB - Monolayer cell cultures of calcitonin secreting cells have been derived from trout ultimobranchial glands. These cultures have been used to study calcitonin secretion and its regulation in vitro; calcitonin has been measured by radioimmunoassay. The cultures exhibit linear hormonal secretion rates and secretory responses to several substances at concentrations as low as 10-8M. Pancreozymin, prostaglandins and dibutyryl cAMP cause at least a 5 fold increase in calcitonin secretion; calcium has a small secretory effect. This system affords a unique method for the study of both the extracellular and intracellular factors involved in the regulation of calcitonin secretion.

UR - http://www.scopus.com/inward/record.url?scp=0016293980&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016293980&partnerID=8YFLogxK

U2 - 10.1210/endo-95-4-1142

DO - 10.1210/endo-95-4-1142

M3 - Article

C2 - 4369822

AN - SCOPUS:0016293980

VL - 95

SP - 1142

EP - 1149

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 4

ER -

Calcitonin secretion in vitro. I. preparation of monolayer c-cell cultures

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this