Abstract
Monolayer cell cultures of calcitonin secreting cells have been derived from trout ultimobranchial glands. These cultures have been used to study calcitonin secretion and its regulation in vitro; calcitonin has been measured by radioimmunoassay. The cultures exhibit linear hormonal secretion rates and secretory responses to several substances at concentrations as low as 10-8M. Pancreozymin, prostaglandins and dibutyryl cAMP cause at least a 5 fold increase in calcitonin secretion; calcium has a small secretory effect. This system affords a unique method for the study of both the extracellular and intracellular factors involved in the regulation of calcitonin secretion.
Original language | English (US) |
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Pages (from-to) | 1142-1149 |
Number of pages | 8 |
Journal | Endocrinology |
Volume | 95 |
Issue number | 4 |
DOIs | |
State | Published - Jan 1 1974 |
ASJC Scopus subject areas
- Endocrinology