Calcitonin secretion in vitro. I. Preparation of monolayer C cell cultures

B. A. Roos, L. L. Bundy, R. Bailey, L. J. Deftos

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Monolayer cell cultures of calcitonin secreting cells have been derived from trout ultimobranchial glands. These cultures have been used to study calcitonin secretion and its regulation in vitro; calcitonin has been measured by radioimmunoassay. The cultures exhibit linear hormonal secretion rates and secretory responses to several substances at concentrations as low as 10-8M. Pancreozymin, prostaglandins and dibutyryl cAMP cause at least a 5 fold increase in calcitonin secretion; calcium has a small secretory effect. This system affords a unique method for the study of both the extracellular and intracellular factors involved in the regulation of calcitonin secretion.

Original languageEnglish
Pages (from-to)1142-1149
Number of pages8
JournalEndocrinology
Volume95
Issue number4
StatePublished - Dec 1 1974
Externally publishedYes

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Calcitonin
Cell Culture Techniques
Secretory Rate
Trout
Cholecystokinin
Prostaglandins
Radioimmunoassay
In Vitro Techniques
Calcium

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Roos, B. A., Bundy, L. L., Bailey, R., & Deftos, L. J. (1974). Calcitonin secretion in vitro. I. Preparation of monolayer C cell cultures. Endocrinology, 95(4), 1142-1149.

Calcitonin secretion in vitro. I. Preparation of monolayer C cell cultures. / Roos, B. A.; Bundy, L. L.; Bailey, R.; Deftos, L. J.

In: Endocrinology, Vol. 95, No. 4, 01.12.1974, p. 1142-1149.

Research output: Contribution to journalArticle

Roos, BA, Bundy, LL, Bailey, R & Deftos, LJ 1974, 'Calcitonin secretion in vitro. I. Preparation of monolayer C cell cultures', Endocrinology, vol. 95, no. 4, pp. 1142-1149.
Roos BA, Bundy LL, Bailey R, Deftos LJ. Calcitonin secretion in vitro. I. Preparation of monolayer C cell cultures. Endocrinology. 1974 Dec 1;95(4):1142-1149.
Roos, B. A. ; Bundy, L. L. ; Bailey, R. ; Deftos, L. J. / Calcitonin secretion in vitro. I. Preparation of monolayer C cell cultures. In: Endocrinology. 1974 ; Vol. 95, No. 4. pp. 1142-1149.
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