Calcitonin secretion in vitro. I. preparation of monolayer c-cell cultures

B. A. Roos; L. L. Bundy; R. Bailey; L. J. Deftos

doi:10.1210/endo-95-4-1142

Calcitonin secretion in vitro. I. preparation of monolayer c-cell cultures

B. A. Roos, L. L. Bundy, R. Bailey, L. J. Deftos

Medicine

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Monolayer cell cultures of calcitonin secreting cells have been derived from trout ultimobranchial glands. These cultures have been used to study calcitonin secretion and its regulation in vitro; calcitonin has been measured by radioimmunoassay. The cultures exhibit linear hormonal secretion rates and secretory responses to several substances at concentrations as low as 10^-8M. Pancreozymin, prostaglandins and dibutyryl cAMP cause at least a 5 fold increase in calcitonin secretion; calcium has a small secretory effect. This system affords a unique method for the study of both the extracellular and intracellular factors involved in the regulation of calcitonin secretion.

Original language	English (US)
Pages (from-to)	1142-1149
Number of pages	8
Journal	Endocrinology
Volume	95
Issue number	4
DOIs	https://doi.org/10.1210/endo-95-4-1142
State	Published - Jan 1 1974

ASJC Scopus subject areas

Endocrinology

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AU - Roos, B. A.

AU - Bundy, L. L.

AU - Bailey, R.

AU - Deftos, L. J.

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AB - Monolayer cell cultures of calcitonin secreting cells have been derived from trout ultimobranchial glands. These cultures have been used to study calcitonin secretion and its regulation in vitro; calcitonin has been measured by radioimmunoassay. The cultures exhibit linear hormonal secretion rates and secretory responses to several substances at concentrations as low as 10-8M. Pancreozymin, prostaglandins and dibutyryl cAMP cause at least a 5 fold increase in calcitonin secretion; calcium has a small secretory effect. This system affords a unique method for the study of both the extracellular and intracellular factors involved in the regulation of calcitonin secretion.

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