Calcitonin secretion by monolayer cultures of human C cells derived from medullary thyroid carcinoma

B. A. Roos, L. L. Bundy, E. A. Miller, L. J. Deftos

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Monolayer cultures of human calcitonin secreting cells (C cells) have been derived from medullary thyroid carcinomas. The cultures were prepared from cell suspensions obtained by enzymatic digestion of surgical specimens of the tumor. Human calcitonin (hCT) secretion by these cells was studied using a specific radioimmunoassay for the hormone. The cultures could be used for reproducible secretion studies up to 40 days after their initiation; they demonstrated a linear rate of hormone secretion in the basal state for at least 4 h. Calcium, pentagastrin, and prostaglandin E2 (PGE2) each produced a marked increase (up to 7 fold) in hormone secretion. Magnesium had no apparent secretory effect; and compared to PGE2, PGF2(α) had only a small secretory effect. In addition to responding to specific secretagogues shown to regulate calcitonin secretion in vivo, the secretory effects of each of the secretion in vivo, the secretory effects of each of the secretagogues could be rapidly reversed. Therefore, these cultures of human C cells exhibit secretory responses which are quantitatively and qualitatively similar to C cells in vivo. Accordingly, such cultures provide a useful model to study the regulation of calcitonin secretion in human C cells.

Original languageEnglish
Pages (from-to)39-45
Number of pages7
JournalEndocrinology
Volume97
Issue number1
StatePublished - Dec 1 1975
Externally publishedYes

Fingerprint

Calcitonin
Hormones
Dinoprostone
Pentagastrin
Dinoprost
Medullary Thyroid cancer
Magnesium
Radioimmunoassay
Digestion
Suspensions
Calcium
Neoplasms

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Roos, B. A., Bundy, L. L., Miller, E. A., & Deftos, L. J. (1975). Calcitonin secretion by monolayer cultures of human C cells derived from medullary thyroid carcinoma. Endocrinology, 97(1), 39-45.

Calcitonin secretion by monolayer cultures of human C cells derived from medullary thyroid carcinoma. / Roos, B. A.; Bundy, L. L.; Miller, E. A.; Deftos, L. J.

In: Endocrinology, Vol. 97, No. 1, 01.12.1975, p. 39-45.

Research output: Contribution to journalArticle

Roos, BA, Bundy, LL, Miller, EA & Deftos, LJ 1975, 'Calcitonin secretion by monolayer cultures of human C cells derived from medullary thyroid carcinoma', Endocrinology, vol. 97, no. 1, pp. 39-45.
Roos, B. A. ; Bundy, L. L. ; Miller, E. A. ; Deftos, L. J. / Calcitonin secretion by monolayer cultures of human C cells derived from medullary thyroid carcinoma. In: Endocrinology. 1975 ; Vol. 97, No. 1. pp. 39-45.
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