Abstract
Monolayer cultures of human calcitonin secreting cells (C cells) have been derived from medullary thyroid carcinomas. The cultures were prepared from cell suspensions obtained by enzymatic digestion of surgical specimens of the tumor. Human calcitonin (hCT) secretion by these cells was studied using a specific radioimmunoassay for the hormone. The cultures could be used for reproducible secretion studies up to 40 days after their initiation; they demonstrated a linear rate of hormone secretion in the basal state for at least 4 h. Calcium, pentagastrin, and prostaglandin E2 (PGE2) each produced a marked increase (up to 7 fold) in hormone secretion. Magnesium had no apparent secretory effect; and compared to PGE2, PGF2(α) had only a small secretory effect. In addition to responding to specific secretagogues shown to regulate calcitonin secretion in vivo, the secretory effects of each of the secretion in vivo, the secretory effects of each of the secretagogues could be rapidly reversed. Therefore, these cultures of human C cells exhibit secretory responses which are quantitatively and qualitatively similar to C cells in vivo. Accordingly, such cultures provide a useful model to study the regulation of calcitonin secretion in human C cells.
| Original language | English |
|---|---|
| Pages (from-to) | 39-45 |
| Number of pages | 7 |
| Journal | Endocrinology |
| Volume | 97 |
| Issue number | 1 |
| State | Published - Dec 1 1975 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Endocrinology
- Endocrinology, Diabetes and Metabolism
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Calcitonin secretion by monolayer cultures of human C cells derived from medullary thyroid carcinoma. / Roos, B. A.; Bundy, L. L.; Miller, E. A.; Deftos, L. J.
In: Endocrinology, Vol. 97, No. 1, 01.12.1975, p. 39-45.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Calcitonin secretion by monolayer cultures of human C cells derived from medullary thyroid carcinoma
AU - Roos, B. A.
AU - Bundy, L. L.
AU - Miller, E. A.
AU - Deftos, L. J.
PY - 1975/12/1
Y1 - 1975/12/1
N2 - Monolayer cultures of human calcitonin secreting cells (C cells) have been derived from medullary thyroid carcinomas. The cultures were prepared from cell suspensions obtained by enzymatic digestion of surgical specimens of the tumor. Human calcitonin (hCT) secretion by these cells was studied using a specific radioimmunoassay for the hormone. The cultures could be used for reproducible secretion studies up to 40 days after their initiation; they demonstrated a linear rate of hormone secretion in the basal state for at least 4 h. Calcium, pentagastrin, and prostaglandin E2 (PGE2) each produced a marked increase (up to 7 fold) in hormone secretion. Magnesium had no apparent secretory effect; and compared to PGE2, PGF2(α) had only a small secretory effect. In addition to responding to specific secretagogues shown to regulate calcitonin secretion in vivo, the secretory effects of each of the secretion in vivo, the secretory effects of each of the secretagogues could be rapidly reversed. Therefore, these cultures of human C cells exhibit secretory responses which are quantitatively and qualitatively similar to C cells in vivo. Accordingly, such cultures provide a useful model to study the regulation of calcitonin secretion in human C cells.
AB - Monolayer cultures of human calcitonin secreting cells (C cells) have been derived from medullary thyroid carcinomas. The cultures were prepared from cell suspensions obtained by enzymatic digestion of surgical specimens of the tumor. Human calcitonin (hCT) secretion by these cells was studied using a specific radioimmunoassay for the hormone. The cultures could be used for reproducible secretion studies up to 40 days after their initiation; they demonstrated a linear rate of hormone secretion in the basal state for at least 4 h. Calcium, pentagastrin, and prostaglandin E2 (PGE2) each produced a marked increase (up to 7 fold) in hormone secretion. Magnesium had no apparent secretory effect; and compared to PGE2, PGF2(α) had only a small secretory effect. In addition to responding to specific secretagogues shown to regulate calcitonin secretion in vivo, the secretory effects of each of the secretion in vivo, the secretory effects of each of the secretagogues could be rapidly reversed. Therefore, these cultures of human C cells exhibit secretory responses which are quantitatively and qualitatively similar to C cells in vivo. Accordingly, such cultures provide a useful model to study the regulation of calcitonin secretion in human C cells.
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M3 - Article
C2 - 1140173
AN - SCOPUS:0016734654
VL - 97
SP - 39
EP - 45
JO - Endocrinology
JF - Endocrinology
SN - 0013-7227
IS - 1
ER -