Calcitonin gene-related peptide (CGRP) may award relative protection from interferon-γ-induced collapse of human hair follicle immune privilege

Michael Kinori, Marta Bertolini, Wolfgang Funk, Liat Samuelov, Katja C. Meyer, Vladimir U. Emelianov, Sybille Hasse, Ralf Paus

Research output: Contribution to journalLetter

19 Citations (Scopus)

Abstract

Interferon-γ (IFNγ)-induced collapse of hair follicle (HF) immune privilege (IP) is a key element in the pathogenesis of alopecia areata. In this pilot study, we investigated whether the immunosuppressive neuropeptide, calcitonin gene-related peptide (CGRP), can protect from and/or restore IFNγ-induced HF-IP collapse. After showing that human scalp HFs express CGRP receptor-like receptor (CRLR) immunoreactivity, anagen HFs were cultured in the presence of IFNγ, with CGRP added before or after. Adding CGRP after IFNγ administration ('restoration assay') failed to downregulate IFNγ-induced ectopic MHC class I expression, while MHC class II expression was reduced. However, administering CGRP before IFNγ application ('protection assay') significantly reduced the IFNγ-induced overexpression and ectopic expression of MHC class I and II and reduced the increased degranulation of perifollicular mast cells induced by IFNγ. This suggests that CGRP may not restore HF-IP once it has collapsed, but may protect it from collapsing. Therefore, CRLR stimulation might help to retard AA progression.

Original languageEnglish (US)
Pages (from-to)223-226
Number of pages4
JournalExperimental Dermatology
Volume21
Issue number3
DOIs
StatePublished - Mar 1 2012
Externally publishedYes

Fingerprint

Hair Follicle
Calcitonin Gene-Related Peptide
Interferons
Calcitonin Gene-Related Peptide Receptors
Assays
Alopecia Areata
Immunosuppressive Agents
Scalp
Neuropeptides
Mast Cells
Restoration
Down-Regulation

Keywords

  • Hair follicle
  • Immune privilege

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

Calcitonin gene-related peptide (CGRP) may award relative protection from interferon-γ-induced collapse of human hair follicle immune privilege. / Kinori, Michael; Bertolini, Marta; Funk, Wolfgang; Samuelov, Liat; Meyer, Katja C.; Emelianov, Vladimir U.; Hasse, Sybille; Paus, Ralf.

In: Experimental Dermatology, Vol. 21, No. 3, 01.03.2012, p. 223-226.

Research output: Contribution to journalLetter

Kinori, Michael ; Bertolini, Marta ; Funk, Wolfgang ; Samuelov, Liat ; Meyer, Katja C. ; Emelianov, Vladimir U. ; Hasse, Sybille ; Paus, Ralf. / Calcitonin gene-related peptide (CGRP) may award relative protection from interferon-γ-induced collapse of human hair follicle immune privilege. In: Experimental Dermatology. 2012 ; Vol. 21, No. 3. pp. 223-226.
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