Cadmium-induced postaxial forelimb ectrodactyly

Association with altered sonic hedgehog signaling

William J. Scott, Claire M. Schreiner, John A. Goetz, David J Robbins, Sheila M. Bell

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Administration of CdSO4 to C57BL/6 mice at day 9.5 of gestation induces a high incidence of postaxial forelimb ectrodactyly in the offspring. We propose that Cd2+ exposure impairs the process of anterior/posterior formation in the limb bud, a process that is directed by Sonic hedgehog (Shh) signaling. We show that exposure of the mouse embryo to Cd2+ disrupts Shh signaling as measured by polarizing activity of mouse limb bud ZPA grafted to a host chick wing, and activity of a Gli:luciferase reporter exposed to limb bud lysates. Yet the expression of Shh and its translation are not affected by Cd2+ exposure. We propose that teratogen exposure affects the processing of Shh in the cells in which it is made.

Original languageEnglish
Pages (from-to)479-485
Number of pages7
JournalReproductive Toxicology
Volume19
Issue number4
DOIs
StatePublished - Mar 1 2005
Externally publishedYes

Fingerprint

Hedgehogs
Forelimb
Cadmium
Limb Buds
Teratogens
Luciferases
Processing
Inbred C57BL Mouse
Embryonic Structures
Pregnancy
Ectrodactyly
Incidence

Keywords

  • Cadmium
  • Fgf8
  • Gli/luciferase reporter
  • Gli3
  • Patched
  • Polarizing activity
  • Postaxial forelimb ectrodactyly
  • Sonic hedgehog

ASJC Scopus subject areas

  • Toxicology

Cite this

Cadmium-induced postaxial forelimb ectrodactyly : Association with altered sonic hedgehog signaling. / Scott, William J.; Schreiner, Claire M.; Goetz, John A.; Robbins, David J; Bell, Sheila M.

In: Reproductive Toxicology, Vol. 19, No. 4, 01.03.2005, p. 479-485.

Research output: Contribution to journalArticle

Scott, William J. ; Schreiner, Claire M. ; Goetz, John A. ; Robbins, David J ; Bell, Sheila M. / Cadmium-induced postaxial forelimb ectrodactyly : Association with altered sonic hedgehog signaling. In: Reproductive Toxicology. 2005 ; Vol. 19, No. 4. pp. 479-485.
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