Cadmium disrupts behavioural and physiological responses to alarm substance in juvenile rainbow trout (Oncorhynchus mykiss)

Graham R. Scott, Katherine A. Sloman, Claude Rouleau, Chris M. Wood

Research output: Contribution to journalArticlepeer-review

148 Scopus citations

Abstract

Alarm substance is a chemical signal released from fish skin epithelial cells after a predator causes skin damage. When other prey fish detect alarm substance by olfaction, they perform stereotypical predator-avoidance behaviours to decrease predation risk. The objective of this study was to explore the effect of sublethal cadmium (Cd) exposure on the behavioural and physiological responses of juvenile rainbow trout (Oncorhynchus mykiss) to alarm substance. Waterborne exposure to 2 μg Cd l-1 for 7 days eliminated normal antipredator behaviours exhibited in response to alarm substance, whereas exposures of shorter duration or lower concentration had no effect on normal behaviour. Furthermore, dietary exposure to 3 μg Cd g-1 in the food for 7 days, which produced the same whole-body Cd accumulation as waterborne exposure to 2 μg l-1, did not alter normal behaviour, indicating that an effect specific to waterborne exposure alone (i.e. Cd accumulation in the olfactory system) results in behavioural alteration. Wholebody phosphor screen autoradiography of fish exposed to 109Cd demonstrated that Cd deposition in the olfactory system (rosette, nerve and bulb) during waterborne exposure was greater than in all other organs of accumulation except the gill. However, Cd could not be detected in the brain. A short-term elevation in plasma cortisol occurred in response to alarm substance under control conditions. Cd exposures of 2 μg l-1 waterborne and 3 μg g-1 dietary for 7 days both inhibited this plasma cortisol elevation but did not alter baseline cortisol levels. Our results suggest that exposure to waterborne Cd at environmentally realistic levels (2 μg l-1) can disrupt the normal behavioural and physiological responses of fish to alarm substance and can thereby alter predator-avoidance strategies, with potential impacts on aquatic fish communities.

Original languageEnglish (US)
Pages (from-to)1779-1790
Number of pages12
JournalJournal of Experimental Biology
Volume206
Issue number11
DOIs
StatePublished - Jun 2003

Keywords

  • Alarm pheromone
  • Behaviour
  • Cortisol
  • Fish
  • Metal
  • Olfaction
  • Oncorhynchus mykiss
  • Predator avoidance
  • Quantitative autoradiography

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

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