Cadmium accumulation and in vitro analysis of calcium and cadmium transport functions in the gastro-intestinal tract of trout following chronic dietary cadmium and calcium feeding

Joel S. Klinck, Tania Y T Ng, Chris M. Wood

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Juvenile rainbow trout (Oncorhynchus mykiss) were fed diets made from Lumbriculus variegatus containing environmentally relevant concentrations of Cd (∼ 0.2 and 12 μg g- 1 dry wt) and/or Ca (1, 10, 20 and 60 mg g- 1 dry wt) for 4 weeks. Ten fish per treatment were removed weekly for tissue metal burden analysis. In all portions of the gastro-intestinal tract (GIT) (stomach, anterior, mid, and posterior intestine), chronic exposure to elevated dietary Ca decreased Cd tissue accumulation to varying degrees. At week five, the GITs of the remaining fish were subjected to an in vitro gut sac technique. Pre-exposure to the different treatments affected unidirectional uptake and binding rates of Cd and Ca in different manners, dependent on the specific GIT section. Ca and Cd uptake rates were highly correlated within all sections of the GIT, and the loosely binding rate of Cd to the GIT surfaces predicted the rate of new Cd absorption. Overall, this study indicates that elevated dietary Ca is protective against Cd uptake from an environmentally relevant diet, and that Ca and Cd uptake may occur through both common and separate pathways in the GIT.

Original languageEnglish
Pages (from-to)349-360
Number of pages12
JournalComparative Biochemistry and Physiology - C Toxicology and Pharmacology
Volume150
Issue number3
DOIs
StatePublished - Sep 1 2009

Fingerprint

Dietary Calcium
Trout
Oncorhynchus mykiss
Nutrition
Cadmium
Fish
Fishes
Tissue
Diet
Calcium
Intestines
Stomach
Metals
In Vitro Techniques

Keywords

  • Biotic ligand model
  • Cadmium
  • Calcium
  • Chronic
  • Diet
  • Gastro-intestinal tract
  • Lumbriculus variegatus
  • Oncorhynchus mykiss

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Physiology
  • Health, Toxicology and Mutagenesis
  • Toxicology

Cite this

@article{79cd7e76ea874b32ace3e64230c389e0,
title = "Cadmium accumulation and in vitro analysis of calcium and cadmium transport functions in the gastro-intestinal tract of trout following chronic dietary cadmium and calcium feeding",
abstract = "Juvenile rainbow trout (Oncorhynchus mykiss) were fed diets made from Lumbriculus variegatus containing environmentally relevant concentrations of Cd (∼ 0.2 and 12 μg g- 1 dry wt) and/or Ca (1, 10, 20 and 60 mg g- 1 dry wt) for 4 weeks. Ten fish per treatment were removed weekly for tissue metal burden analysis. In all portions of the gastro-intestinal tract (GIT) (stomach, anterior, mid, and posterior intestine), chronic exposure to elevated dietary Ca decreased Cd tissue accumulation to varying degrees. At week five, the GITs of the remaining fish were subjected to an in vitro gut sac technique. Pre-exposure to the different treatments affected unidirectional uptake and binding rates of Cd and Ca in different manners, dependent on the specific GIT section. Ca and Cd uptake rates were highly correlated within all sections of the GIT, and the loosely binding rate of Cd to the GIT surfaces predicted the rate of new Cd absorption. Overall, this study indicates that elevated dietary Ca is protective against Cd uptake from an environmentally relevant diet, and that Ca and Cd uptake may occur through both common and separate pathways in the GIT.",
keywords = "Biotic ligand model, Cadmium, Calcium, Chronic, Diet, Gastro-intestinal tract, Lumbriculus variegatus, Oncorhynchus mykiss",
author = "Klinck, {Joel S.} and Ng, {Tania Y T} and Wood, {Chris M.}",
year = "2009",
month = "9",
day = "1",
doi = "10.1016/j.cbpc.2009.05.018",
language = "English",
volume = "150",
pages = "349--360",
journal = "Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology",
issn = "1532-0456",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Cadmium accumulation and in vitro analysis of calcium and cadmium transport functions in the gastro-intestinal tract of trout following chronic dietary cadmium and calcium feeding

AU - Klinck, Joel S.

AU - Ng, Tania Y T

AU - Wood, Chris M.

PY - 2009/9/1

Y1 - 2009/9/1

N2 - Juvenile rainbow trout (Oncorhynchus mykiss) were fed diets made from Lumbriculus variegatus containing environmentally relevant concentrations of Cd (∼ 0.2 and 12 μg g- 1 dry wt) and/or Ca (1, 10, 20 and 60 mg g- 1 dry wt) for 4 weeks. Ten fish per treatment were removed weekly for tissue metal burden analysis. In all portions of the gastro-intestinal tract (GIT) (stomach, anterior, mid, and posterior intestine), chronic exposure to elevated dietary Ca decreased Cd tissue accumulation to varying degrees. At week five, the GITs of the remaining fish were subjected to an in vitro gut sac technique. Pre-exposure to the different treatments affected unidirectional uptake and binding rates of Cd and Ca in different manners, dependent on the specific GIT section. Ca and Cd uptake rates were highly correlated within all sections of the GIT, and the loosely binding rate of Cd to the GIT surfaces predicted the rate of new Cd absorption. Overall, this study indicates that elevated dietary Ca is protective against Cd uptake from an environmentally relevant diet, and that Ca and Cd uptake may occur through both common and separate pathways in the GIT.

AB - Juvenile rainbow trout (Oncorhynchus mykiss) were fed diets made from Lumbriculus variegatus containing environmentally relevant concentrations of Cd (∼ 0.2 and 12 μg g- 1 dry wt) and/or Ca (1, 10, 20 and 60 mg g- 1 dry wt) for 4 weeks. Ten fish per treatment were removed weekly for tissue metal burden analysis. In all portions of the gastro-intestinal tract (GIT) (stomach, anterior, mid, and posterior intestine), chronic exposure to elevated dietary Ca decreased Cd tissue accumulation to varying degrees. At week five, the GITs of the remaining fish were subjected to an in vitro gut sac technique. Pre-exposure to the different treatments affected unidirectional uptake and binding rates of Cd and Ca in different manners, dependent on the specific GIT section. Ca and Cd uptake rates were highly correlated within all sections of the GIT, and the loosely binding rate of Cd to the GIT surfaces predicted the rate of new Cd absorption. Overall, this study indicates that elevated dietary Ca is protective against Cd uptake from an environmentally relevant diet, and that Ca and Cd uptake may occur through both common and separate pathways in the GIT.

KW - Biotic ligand model

KW - Cadmium

KW - Calcium

KW - Chronic

KW - Diet

KW - Gastro-intestinal tract

KW - Lumbriculus variegatus

KW - Oncorhynchus mykiss

UR - http://www.scopus.com/inward/record.url?scp=67650717218&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67650717218&partnerID=8YFLogxK

U2 - 10.1016/j.cbpc.2009.05.018

DO - 10.1016/j.cbpc.2009.05.018

M3 - Article

C2 - 19527800

AN - SCOPUS:67650717218

VL - 150

SP - 349

EP - 360

JO - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology

JF - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology

SN - 1532-0456

IS - 3

ER -