C1q

Isolation from human serum in high yield by affinity chromatography and development of a highly sensitive hemolytic assay

W. P. Kolb, L. M. Kolb, E. R. Podack

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

C1q, a subcomponent of the first component of complement, has been isolated from human serum in fully hemolytically active form by affinity column chromatography and gel filtration with Bio Gel A 5M. The affinity column was prepared by covalent coupling of purified human IgG to CNBr-activated Sepharose 4B. Final yields of C1q ranged from 25 to 40% with 650- and 890-fold purification based on recovery of hemolytic activity. The preparations were free of contaminating serum proteins as judged by SDS polyacrylamide gel electrophoretic and immunochemical criteria. The final C1q preparations were also devoid of any demonstrable C1q inhibitor activity. A C1q-depleted reagent (C1qD) was obtained from the nonadsorbed protein containing fractions of the human IgG Sepharose 4B affinity column and utilized in conjunction with sensitized sheep erythrocytes (EA) for the detection and quantitation of C1q hemolytic activity. Employing optimal quantities of C1qD in the hemolytic assay mixture, the highly purified C1q preparations contained 0.5 to 1 x 1013 effective molecules/mg and 0.5 to 1 x 1012 effective C1q molecules/ml of human serum. This assay would therefore reproducibly detect less than 1 ng of C1q hemolytic activity.

Original languageEnglish
Pages (from-to)2103-2111
Number of pages9
JournalJournal of Immunology
Volume122
Issue number5
StatePublished - Dec 1 1979
Externally publishedYes

Fingerprint

Affinity Chromatography
Serum
Sepharose
Gel Chromatography
Blood Proteins
Sheep
Immunoglobulin G
Erythrocytes
Gels
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

C1q : Isolation from human serum in high yield by affinity chromatography and development of a highly sensitive hemolytic assay. / Kolb, W. P.; Kolb, L. M.; Podack, E. R.

In: Journal of Immunology, Vol. 122, No. 5, 01.12.1979, p. 2103-2111.

Research output: Contribution to journalArticle

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AB - C1q, a subcomponent of the first component of complement, has been isolated from human serum in fully hemolytically active form by affinity column chromatography and gel filtration with Bio Gel A 5M. The affinity column was prepared by covalent coupling of purified human IgG to CNBr-activated Sepharose 4B. Final yields of C1q ranged from 25 to 40% with 650- and 890-fold purification based on recovery of hemolytic activity. The preparations were free of contaminating serum proteins as judged by SDS polyacrylamide gel electrophoretic and immunochemical criteria. The final C1q preparations were also devoid of any demonstrable C1q inhibitor activity. A C1q-depleted reagent (C1qD) was obtained from the nonadsorbed protein containing fractions of the human IgG Sepharose 4B affinity column and utilized in conjunction with sensitized sheep erythrocytes (EA) for the detection and quantitation of C1q hemolytic activity. Employing optimal quantities of C1qD in the hemolytic assay mixture, the highly purified C1q preparations contained 0.5 to 1 x 1013 effective molecules/mg and 0.5 to 1 x 1012 effective C1q molecules/ml of human serum. This assay would therefore reproducibly detect less than 1 ng of C1q hemolytic activity.

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