C-type natriuretic peptide receptor expression in pancreatic alpha cells

Matthew D. Burgess, Kim D. Moore, Gay M. Carter, Abdel A. Alli, Christopher S. Granda, Hirohito Ichii, Camillo Ricordi, William R. Gower

Research output: Contribution to journalArticle

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Abstract

Atrial natriuretic peptide (ANP), brain type natriuretic peptide (BNP) and C-type natriuretic peptide (CNP) comprise a family of natriuretic peptides that mediate their biological effects through three natriuretic peptide receptor subtypes, NPR-A (ANP, BNP), NPR-B (CNP) and NPR-C (ANP, BNP, CNP). Several reports have provided evidence for the expression of ANP and specific binding sites for ANP in the pancreas. The purpose of this study was to identify the ANP receptor subtype and to localize its expression to a specific cell type in the human pancreas. NPR-C immunoreactivity, but neither ANP nor NPR-A, was detected in human islets by immunofluorescent staining. No immunostaining was observed in the exocrine pancreas or ductal structures. Double-staining revealed that NPR-C was expressed mainly in the glucagon-containing alpha cells. NPR-C mRNA and protein were detected in isolated human islets by RT-PCR and Western blot analysis, respectively. NPR-C expression was also detected by immunofluorescent staining in glucagonoma but not in insulinoma. ANP, as well as BNP and CNP, stimulated glucagon secretion from perifused human islets (1,111 ± 55% vs. basal [7.3 fmol/min]; P < 0.001). This response was mimicked by cANP(4-23), a selective agonist of NPR-C. In conclusion, the NPR-C receptor is expressed in normal and neoplastic human alpha cells. These findings suggest a role for natriuretic peptides in the regulation of glucagon secretion from human alpha cells.

Original languageEnglish
Pages (from-to)95-103
Number of pages9
JournalHistochemistry and Cell Biology
Volume132
Issue number1
DOIs
StatePublished - Jul 1 2009

Fingerprint

Glucagon-Secreting Cells
C-Type Natriuretic Peptide
Atrial Natriuretic Factor
Brain Natriuretic Peptide
Natriuretic Peptides
Glucagon
Staining and Labeling
Pancreas
Glucagonoma
Atrial Natriuretic Factor Receptors
Exocrine Pancreas
Insulinoma
Peptide Receptors
Protein C
atrial natriuretic factor receptor C
Western Blotting
Binding Sites
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Alpha cells
  • Anp
  • Glucagon
  • Islets of langerhans
  • Npr-c
  • Pancreas

ASJC Scopus subject areas

  • Cell Biology
  • Histology
  • Medical Laboratory Technology
  • Molecular Biology

Cite this

Burgess, M. D., Moore, K. D., Carter, G. M., Alli, A. A., Granda, C. S., Ichii, H., ... Gower, W. R. (2009). C-type natriuretic peptide receptor expression in pancreatic alpha cells. Histochemistry and Cell Biology, 132(1), 95-103. https://doi.org/10.1007/s00418-009-0591-3

C-type natriuretic peptide receptor expression in pancreatic alpha cells. / Burgess, Matthew D.; Moore, Kim D.; Carter, Gay M.; Alli, Abdel A.; Granda, Christopher S.; Ichii, Hirohito; Ricordi, Camillo; Gower, William R.

In: Histochemistry and Cell Biology, Vol. 132, No. 1, 01.07.2009, p. 95-103.

Research output: Contribution to journalArticle

Burgess, MD, Moore, KD, Carter, GM, Alli, AA, Granda, CS, Ichii, H, Ricordi, C & Gower, WR 2009, 'C-type natriuretic peptide receptor expression in pancreatic alpha cells', Histochemistry and Cell Biology, vol. 132, no. 1, pp. 95-103. https://doi.org/10.1007/s00418-009-0591-3
Burgess MD, Moore KD, Carter GM, Alli AA, Granda CS, Ichii H et al. C-type natriuretic peptide receptor expression in pancreatic alpha cells. Histochemistry and Cell Biology. 2009 Jul 1;132(1):95-103. https://doi.org/10.1007/s00418-009-0591-3
Burgess, Matthew D. ; Moore, Kim D. ; Carter, Gay M. ; Alli, Abdel A. ; Granda, Christopher S. ; Ichii, Hirohito ; Ricordi, Camillo ; Gower, William R. / C-type natriuretic peptide receptor expression in pancreatic alpha cells. In: Histochemistry and Cell Biology. 2009 ; Vol. 132, No. 1. pp. 95-103.
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