Abstract
Atrial natriuretic peptide (ANP), brain type natriuretic peptide (BNP) and C-type natriuretic peptide (CNP) comprise a family of natriuretic peptides that mediate their biological effects through three natriuretic peptide receptor subtypes, NPR-A (ANP, BNP), NPR-B (CNP) and NPR-C (ANP, BNP, CNP). Several reports have provided evidence for the expression of ANP and specific binding sites for ANP in the pancreas. The purpose of this study was to identify the ANP receptor subtype and to localize its expression to a specific cell type in the human pancreas. NPR-C immunoreactivity, but neither ANP nor NPR-A, was detected in human islets by immunofluorescent staining. No immunostaining was observed in the exocrine pancreas or ductal structures. Double-staining revealed that NPR-C was expressed mainly in the glucagon-containing alpha cells. NPR-C mRNA and protein were detected in isolated human islets by RT-PCR and Western blot analysis, respectively. NPR-C expression was also detected by immunofluorescent staining in glucagonoma but not in insulinoma. ANP, as well as BNP and CNP, stimulated glucagon secretion from perifused human islets (1,111 ± 55% vs. basal [7.3 fmol/min]; P < 0.001). This response was mimicked by cANP(4-23), a selective agonist of NPR-C. In conclusion, the NPR-C receptor is expressed in normal and neoplastic human alpha cells. These findings suggest a role for natriuretic peptides in the regulation of glucagon secretion from human alpha cells.
| Original language | English |
|---|---|
| Pages (from-to) | 95-103 |
| Number of pages | 9 |
| Journal | Histochemistry and Cell Biology |
| Volume | 132 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 1 2009 |
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Keywords
- Alpha cells
- Anp
- Glucagon
- Islets of langerhans
- Npr-c
- Pancreas
ASJC Scopus subject areas
- Cell Biology
- Histology
- Medical Laboratory Technology
- Molecular Biology
Cite this
C-type natriuretic peptide receptor expression in pancreatic alpha cells. / Burgess, Matthew D.; Moore, Kim D.; Carter, Gay M.; Alli, Abdel A.; Granda, Christopher S.; Ichii, Hirohito; Ricordi, Camillo; Gower, William R.
In: Histochemistry and Cell Biology, Vol. 132, No. 1, 01.07.2009, p. 95-103.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - C-type natriuretic peptide receptor expression in pancreatic alpha cells
AU - Burgess, Matthew D.
AU - Moore, Kim D.
AU - Carter, Gay M.
AU - Alli, Abdel A.
AU - Granda, Christopher S.
AU - Ichii, Hirohito
AU - Ricordi, Camillo
AU - Gower, William R.
PY - 2009/7/1
Y1 - 2009/7/1
N2 - Atrial natriuretic peptide (ANP), brain type natriuretic peptide (BNP) and C-type natriuretic peptide (CNP) comprise a family of natriuretic peptides that mediate their biological effects through three natriuretic peptide receptor subtypes, NPR-A (ANP, BNP), NPR-B (CNP) and NPR-C (ANP, BNP, CNP). Several reports have provided evidence for the expression of ANP and specific binding sites for ANP in the pancreas. The purpose of this study was to identify the ANP receptor subtype and to localize its expression to a specific cell type in the human pancreas. NPR-C immunoreactivity, but neither ANP nor NPR-A, was detected in human islets by immunofluorescent staining. No immunostaining was observed in the exocrine pancreas or ductal structures. Double-staining revealed that NPR-C was expressed mainly in the glucagon-containing alpha cells. NPR-C mRNA and protein were detected in isolated human islets by RT-PCR and Western blot analysis, respectively. NPR-C expression was also detected by immunofluorescent staining in glucagonoma but not in insulinoma. ANP, as well as BNP and CNP, stimulated glucagon secretion from perifused human islets (1,111 ± 55% vs. basal [7.3 fmol/min]; P < 0.001). This response was mimicked by cANP(4-23), a selective agonist of NPR-C. In conclusion, the NPR-C receptor is expressed in normal and neoplastic human alpha cells. These findings suggest a role for natriuretic peptides in the regulation of glucagon secretion from human alpha cells.
AB - Atrial natriuretic peptide (ANP), brain type natriuretic peptide (BNP) and C-type natriuretic peptide (CNP) comprise a family of natriuretic peptides that mediate their biological effects through three natriuretic peptide receptor subtypes, NPR-A (ANP, BNP), NPR-B (CNP) and NPR-C (ANP, BNP, CNP). Several reports have provided evidence for the expression of ANP and specific binding sites for ANP in the pancreas. The purpose of this study was to identify the ANP receptor subtype and to localize its expression to a specific cell type in the human pancreas. NPR-C immunoreactivity, but neither ANP nor NPR-A, was detected in human islets by immunofluorescent staining. No immunostaining was observed in the exocrine pancreas or ductal structures. Double-staining revealed that NPR-C was expressed mainly in the glucagon-containing alpha cells. NPR-C mRNA and protein were detected in isolated human islets by RT-PCR and Western blot analysis, respectively. NPR-C expression was also detected by immunofluorescent staining in glucagonoma but not in insulinoma. ANP, as well as BNP and CNP, stimulated glucagon secretion from perifused human islets (1,111 ± 55% vs. basal [7.3 fmol/min]; P < 0.001). This response was mimicked by cANP(4-23), a selective agonist of NPR-C. In conclusion, the NPR-C receptor is expressed in normal and neoplastic human alpha cells. These findings suggest a role for natriuretic peptides in the regulation of glucagon secretion from human alpha cells.
KW - Alpha cells
KW - Anp
KW - Glucagon
KW - Islets of langerhans
KW - Npr-c
KW - Pancreas
UR - http://www.scopus.com/inward/record.url?scp=67349255005&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67349255005&partnerID=8YFLogxK
U2 - 10.1007/s00418-009-0591-3
DO - 10.1007/s00418-009-0591-3
M3 - Article
C2 - 19352691
AN - SCOPUS:67349255005
VL - 132
SP - 95
EP - 103
JO - Histochemistry and Cell Biology
JF - Histochemistry and Cell Biology
SN - 0948-6143
IS - 1
ER -