c-Phycocyanin primed silver nano conjugates: Studies on red blood cell stress resilience mechanism

Harishkumar Madhyastha, Radha Madhyastha, Abhishek Thakur, Sakai Kentaro, Abhimanyu Dev, Sneha Singh, Bistivalli Chandrashekharappa R, Hemanth Kumar, Orlando Acevedo, Yuichi Nakajima, Hemant Kumar Daima, Akhela Aradhya, Navya Nagaraj P, Masugi Maruyama

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Green synthesis of metal-encased nutraceutical nano-hybrids has been a target for research over the last few years. In the present investigation, we have reported temperature dependent facile synthesis of silver nanoparticles using FDA approved c phycocyanin (cPC). The cPC conjugated silver nanoparticles (AgcPCNPs) were characterized by TEM, Zeta Potential, UV–vis, XPS, FTIR, and CD Spectroscopy. The temperature optimization studies suggested the synthesis of stable AgcPCNPs at 40 °C while at higher temperature system shows aggregated appearance. Molecular docking studies predicted the exclusive interaction of C, D, I, and J chains of cPC with the surface of AgNPs. Moreover, AgcPCNPs significantly (p < 0.1 %) counteract the toxic nature of AgNPs on red blood cell by measuring parameters like total RBC count, % hemolysis, % hematocrit, coagulation time, pH, electrolyte concentrations and degree of blood cell lipid peroxidation by the anti-oxidation mechanism. Skin fibroblast in vitro cell migration result suggeststhat AgcPCNPs enhanced the degree of cell movement towards the wound area. Data obtained collectively demonstrate that AgcPCNPs can be a better agent in the dermal wound healing with reduced toxicity with the bi-phasic advantage of cPC as a wound healer and Ag nano-metal as an anti-bacterial agent.

Original languageEnglish (US)
Article number111211
JournalColloids and Surfaces B: Biointerfaces
StatePublished - Oct 2020


  • Anti-oxidation
  • Blood electrolytes
  • Conjugated nano-particles
  • Fibroblast cell migration
  • Haemoglobin configuration
  • Silver nanoparticles
  • Wound healing
  • c-Phycocyanin

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry


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