C-kit+ cells isolated from developing kidneys are a novel population of stem cells with regenerative potential

Erika B. Rangel, Samirah A. Gomes, Raul A. Dulce, Courtney Premer, Claudia D Rodrigues, Rosemeire Takeuchi, Behzad Oskouei, Decio A. Carvalho, Phillip Ruiz, Jochen Reiser, Joshua Hare

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

The presence of tissue specific precursor cells is an emerging concept in organ formation and tissue homeostasis. Several progenitors are described in the kidneys. However, their identity as a true stem cell remains elusive. Here, we identify a neonatal kidney-derived c-kit+ cell population that fulfills all of the criteria as a stem cell. These cells were found in the thick ascending limb of Henle's loop and exhibited clonogenicity, self-renewal, and multipotentiality with differentiation capacity into mesoderm and ectoderm progeny. Additionally, c-kit+ cells formed spheres in nonadherent conditions when plated at clonal density and expressed markers of stem cells, progenitors, and differentiated cells. Ex vivo expanded c-kit+ cells integrated into several compartments of the kidney, including tubules, vessels, and glomeruli, and contributed to functional and morphological improvement of the kidney following acute ischemia-reperfusion injury in rats. Together, these findings document a novel neonatal rat kidney c-kit+ stem cell population that can be isolated, expanded, cloned, differentiated, and used for kidney repair following acute kidney injury. These cells have important biological and therapeutic implications.

Original languageEnglish
Pages (from-to)1644-1656
Number of pages13
JournalStem Cells
Volume31
Issue number8
DOIs
StatePublished - Aug 1 2013

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Keywords

  • C-kit
  • Clonogenicity
  • Kidney stem cells
  • Multipotentiality
  • Regenerative potential
  • Self-renewal

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine

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