C. elegans Telomeres Contain G-Strand and C-Strand Overhangs that Are Bound by Distinct Proteins

Marcela Raices, Ramiro E Verdun, Sarah A. Compton, Candy I. Haggblom, Jack D. Griffith, Andrew Dillin, Jan Karlseder

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Single-strand extensions of the G strand of telomeres are known to be critical for chromosome-end protection and length regulation. Here, we report that in C. elegans, chromosome termini possess 3′ G-strand overhangs as well as 5′ C-strand overhangs. C tails are as abundant as G tails and are generated by a well-regulated process. These two classes of overhangs are bound by two single-stranded DNA binding proteins, CeOB1 and CeOB2, which exhibit specificity for G-rich or C-rich telomeric DNA. Strains of worms deleted for CeOB1 have elongated telomeres as well as extended G tails, whereas CeOB2 deficiency leads to telomere-length heterogeneity. Both CeOB1 and CeOB2 contain OB (oligo-saccharide/oligo-nucleotide binding) folds, which exhibit structural similarity to the second and first OB folds of the mammalian telomere binding protein hPOT1, respectively. Our results suggest that C. elegans telomere homeostasis relies on a novel mechanism that involves 5′ and 3′ single-stranded termini.

Original languageEnglish
Pages (from-to)745-757
Number of pages13
JournalCell
Volume132
Issue number5
DOIs
StatePublished - Mar 7 2008
Externally publishedYes

Fingerprint

Telomere
Chromosomes
Telomere-Binding Proteins
Nucleotides
DNA-Binding Proteins
Telomere Homeostasis
Chromosomes, Human, 6-12 and X
Proteins
DNA

Keywords

  • CELLBIO
  • DNA

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Raices, M., Verdun, R. E., Compton, S. A., Haggblom, C. I., Griffith, J. D., Dillin, A., & Karlseder, J. (2008). C. elegans Telomeres Contain G-Strand and C-Strand Overhangs that Are Bound by Distinct Proteins. Cell, 132(5), 745-757. https://doi.org/10.1016/j.cell.2007.12.039

C. elegans Telomeres Contain G-Strand and C-Strand Overhangs that Are Bound by Distinct Proteins. / Raices, Marcela; Verdun, Ramiro E; Compton, Sarah A.; Haggblom, Candy I.; Griffith, Jack D.; Dillin, Andrew; Karlseder, Jan.

In: Cell, Vol. 132, No. 5, 07.03.2008, p. 745-757.

Research output: Contribution to journalArticle

Raices, M, Verdun, RE, Compton, SA, Haggblom, CI, Griffith, JD, Dillin, A & Karlseder, J 2008, 'C. elegans Telomeres Contain G-Strand and C-Strand Overhangs that Are Bound by Distinct Proteins', Cell, vol. 132, no. 5, pp. 745-757. https://doi.org/10.1016/j.cell.2007.12.039
Raices, Marcela ; Verdun, Ramiro E ; Compton, Sarah A. ; Haggblom, Candy I. ; Griffith, Jack D. ; Dillin, Andrew ; Karlseder, Jan. / C. elegans Telomeres Contain G-Strand and C-Strand Overhangs that Are Bound by Distinct Proteins. In: Cell. 2008 ; Vol. 132, No. 5. pp. 745-757.
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