Butyrylcholinesterase in the life cycle of amyloid plaques

A. L. Guillozet, J. F. Smiley, Deborah C Mash, M. M. Mesulam

Research output: Contribution to journalArticle

232 Citations (Scopus)

Abstract

Deposits of diffuse β-amyloid (Aβ) may exist in the brain for many years before leading to neuritic degeneration and dementia. The factors that contribute to the putative transformation of the Aβ amyloid from a relatively inert to a pathogenic state remain unknown and may involve interactions with additional plaque constituents. Matching brain sections from 2 demented and 4 nondemented subjects were processed for the demonstration of Aβ immunoreactivity, butyrylcholinesterase (BChE) enzyme activity, and thioflavine S binding. Additional sections were processed for the concurrent demonstration of two or three of these markers. A comparative analysis of multiple cytoarchitectonic areas processed with each of these markers indicated that Aβ plaque deposits are likely to undergo three stages of maturation, ie, a 'diffuse' thioflavine S-negative stage, a thioflavine S- positive (ie, compact) but nonneuritic stage, and a compact neuritic stage. A multiregional analysis showed that BChE-positive plaques were not found in cytoarchitectonic areas or cortical layers that contained only the thioflavine S-negative, diffuse type of Aβ plaques. The BChE-positive plaques were found only in areas containing thioflavine S-positive compact plaques, both neuritic and nonneuritic. Within such areas, almost all (>98%) BChE-containing plaques bound thioflavine S, and almost all (93%) thioflavine S plaques contained BChE. These results suggest that BChE becomes associated with amyloid plaques at approximately the same time that the Aβ deposit assumes a compact β-pleated conformation. BChE may therefore participate in the transformation of Aβ from an initially benign form to an eventually malignant form associated with neuritic tissue degeneration and clinical dementia.

Original languageEnglish
Pages (from-to)909-918
Number of pages10
JournalAnnals of Neurology
Volume42
Issue number6
DOIs
StatePublished - Dec 1 1997

Fingerprint

Butyrylcholinesterase
Amyloid Plaques
Life Cycle Stages
Dementia
Brain
thioflavin T
Amyloid
Demography
Enzymes

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Guillozet, A. L., Smiley, J. F., Mash, D. C., & Mesulam, M. M. (1997). Butyrylcholinesterase in the life cycle of amyloid plaques. Annals of Neurology, 42(6), 909-918. https://doi.org/10.1002/ana.410420613

Butyrylcholinesterase in the life cycle of amyloid plaques. / Guillozet, A. L.; Smiley, J. F.; Mash, Deborah C; Mesulam, M. M.

In: Annals of Neurology, Vol. 42, No. 6, 01.12.1997, p. 909-918.

Research output: Contribution to journalArticle

Guillozet, AL, Smiley, JF, Mash, DC & Mesulam, MM 1997, 'Butyrylcholinesterase in the life cycle of amyloid plaques', Annals of Neurology, vol. 42, no. 6, pp. 909-918. https://doi.org/10.1002/ana.410420613
Guillozet AL, Smiley JF, Mash DC, Mesulam MM. Butyrylcholinesterase in the life cycle of amyloid plaques. Annals of Neurology. 1997 Dec 1;42(6):909-918. https://doi.org/10.1002/ana.410420613
Guillozet, A. L. ; Smiley, J. F. ; Mash, Deborah C ; Mesulam, M. M. / Butyrylcholinesterase in the life cycle of amyloid plaques. In: Annals of Neurology. 1997 ; Vol. 42, No. 6. pp. 909-918.
@article{b56f7547edd7405bae67d5907c83a8ae,
title = "Butyrylcholinesterase in the life cycle of amyloid plaques",
abstract = "Deposits of diffuse β-amyloid (Aβ) may exist in the brain for many years before leading to neuritic degeneration and dementia. The factors that contribute to the putative transformation of the Aβ amyloid from a relatively inert to a pathogenic state remain unknown and may involve interactions with additional plaque constituents. Matching brain sections from 2 demented and 4 nondemented subjects were processed for the demonstration of Aβ immunoreactivity, butyrylcholinesterase (BChE) enzyme activity, and thioflavine S binding. Additional sections were processed for the concurrent demonstration of two or three of these markers. A comparative analysis of multiple cytoarchitectonic areas processed with each of these markers indicated that Aβ plaque deposits are likely to undergo three stages of maturation, ie, a 'diffuse' thioflavine S-negative stage, a thioflavine S- positive (ie, compact) but nonneuritic stage, and a compact neuritic stage. A multiregional analysis showed that BChE-positive plaques were not found in cytoarchitectonic areas or cortical layers that contained only the thioflavine S-negative, diffuse type of Aβ plaques. The BChE-positive plaques were found only in areas containing thioflavine S-positive compact plaques, both neuritic and nonneuritic. Within such areas, almost all (>98{\%}) BChE-containing plaques bound thioflavine S, and almost all (93{\%}) thioflavine S plaques contained BChE. These results suggest that BChE becomes associated with amyloid plaques at approximately the same time that the Aβ deposit assumes a compact β-pleated conformation. BChE may therefore participate in the transformation of Aβ from an initially benign form to an eventually malignant form associated with neuritic tissue degeneration and clinical dementia.",
author = "Guillozet, {A. L.} and Smiley, {J. F.} and Mash, {Deborah C} and Mesulam, {M. M.}",
year = "1997",
month = "12",
day = "1",
doi = "10.1002/ana.410420613",
language = "English",
volume = "42",
pages = "909--918",
journal = "Annals of Neurology",
issn = "0364-5134",
publisher = "John Wiley and Sons Inc.",
number = "6",

}

TY - JOUR

T1 - Butyrylcholinesterase in the life cycle of amyloid plaques

AU - Guillozet, A. L.

AU - Smiley, J. F.

AU - Mash, Deborah C

AU - Mesulam, M. M.

PY - 1997/12/1

Y1 - 1997/12/1

N2 - Deposits of diffuse β-amyloid (Aβ) may exist in the brain for many years before leading to neuritic degeneration and dementia. The factors that contribute to the putative transformation of the Aβ amyloid from a relatively inert to a pathogenic state remain unknown and may involve interactions with additional plaque constituents. Matching brain sections from 2 demented and 4 nondemented subjects were processed for the demonstration of Aβ immunoreactivity, butyrylcholinesterase (BChE) enzyme activity, and thioflavine S binding. Additional sections were processed for the concurrent demonstration of two or three of these markers. A comparative analysis of multiple cytoarchitectonic areas processed with each of these markers indicated that Aβ plaque deposits are likely to undergo three stages of maturation, ie, a 'diffuse' thioflavine S-negative stage, a thioflavine S- positive (ie, compact) but nonneuritic stage, and a compact neuritic stage. A multiregional analysis showed that BChE-positive plaques were not found in cytoarchitectonic areas or cortical layers that contained only the thioflavine S-negative, diffuse type of Aβ plaques. The BChE-positive plaques were found only in areas containing thioflavine S-positive compact plaques, both neuritic and nonneuritic. Within such areas, almost all (>98%) BChE-containing plaques bound thioflavine S, and almost all (93%) thioflavine S plaques contained BChE. These results suggest that BChE becomes associated with amyloid plaques at approximately the same time that the Aβ deposit assumes a compact β-pleated conformation. BChE may therefore participate in the transformation of Aβ from an initially benign form to an eventually malignant form associated with neuritic tissue degeneration and clinical dementia.

AB - Deposits of diffuse β-amyloid (Aβ) may exist in the brain for many years before leading to neuritic degeneration and dementia. The factors that contribute to the putative transformation of the Aβ amyloid from a relatively inert to a pathogenic state remain unknown and may involve interactions with additional plaque constituents. Matching brain sections from 2 demented and 4 nondemented subjects were processed for the demonstration of Aβ immunoreactivity, butyrylcholinesterase (BChE) enzyme activity, and thioflavine S binding. Additional sections were processed for the concurrent demonstration of two or three of these markers. A comparative analysis of multiple cytoarchitectonic areas processed with each of these markers indicated that Aβ plaque deposits are likely to undergo three stages of maturation, ie, a 'diffuse' thioflavine S-negative stage, a thioflavine S- positive (ie, compact) but nonneuritic stage, and a compact neuritic stage. A multiregional analysis showed that BChE-positive plaques were not found in cytoarchitectonic areas or cortical layers that contained only the thioflavine S-negative, diffuse type of Aβ plaques. The BChE-positive plaques were found only in areas containing thioflavine S-positive compact plaques, both neuritic and nonneuritic. Within such areas, almost all (>98%) BChE-containing plaques bound thioflavine S, and almost all (93%) thioflavine S plaques contained BChE. These results suggest that BChE becomes associated with amyloid plaques at approximately the same time that the Aβ deposit assumes a compact β-pleated conformation. BChE may therefore participate in the transformation of Aβ from an initially benign form to an eventually malignant form associated with neuritic tissue degeneration and clinical dementia.

UR - http://www.scopus.com/inward/record.url?scp=0031458235&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031458235&partnerID=8YFLogxK

U2 - 10.1002/ana.410420613

DO - 10.1002/ana.410420613

M3 - Article

C2 - 9403484

AN - SCOPUS:0031458235

VL - 42

SP - 909

EP - 918

JO - Annals of Neurology

JF - Annals of Neurology

SN - 0364-5134

IS - 6

ER -