Butyrate increases colonocyte protein synthesis in ulcerative colitis

W. Frankel, John Lew, B. Su, A. Bain, D. Klurfeld, E. Einhorn, R. P. MacDermott, J. Rombeau

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Butyrate promotes epithelial cell healing and improves symptoms when administered rectally in patients with distal ulcerative colitis (UC). It was hypothesized that butyrate may enhance healing in patients with UC by stimulating colonocyte proliferation and/or protein production. Mucosa from the descending colon was obtained from patients with UC (n = 5), Crohn's disease (n = 8), diverticulitis (n = 6), and cancer (normal tissue 10 cm from tumor; n = 10). Epithelial cells were isolated using dispase/collagenase and differential sedimentation and incubated for 4 hr at 37°C with either Na butyrate (10 mM) or NaCl (10 mM). Protein synthesis was assessed by [14C]leucine incorporation and proliferation was determined with [3H]thymidine. Mean viability and purity were >88%. Spontaneous proliferation was significantly increased in UC when compared to diverticulitis and normal controls. Butyrate significantly increased protein synthesis in UC epithelial cells when compared to saline control. The therapeutic effects of butyrate in patients with UC may be due to its use by epithelial cells as a metabolic fuel to increase protein production and promote healing.

Original languageEnglish
Pages (from-to)210-214
Number of pages5
JournalJournal of Surgical Research
Volume57
Issue number1
DOIs
StatePublished - Aug 11 1994
Externally publishedYes

Fingerprint

Butyrates
Ulcerative Colitis
Epithelial Cells
Diverticulitis
Proteins
Descending Colon
Therapeutic Uses
Collagenases
Leucine
Crohn Disease
Thymidine
Neoplasms
Mucous Membrane

ASJC Scopus subject areas

  • Surgery

Cite this

Frankel, W., Lew, J., Su, B., Bain, A., Klurfeld, D., Einhorn, E., ... Rombeau, J. (1994). Butyrate increases colonocyte protein synthesis in ulcerative colitis. Journal of Surgical Research, 57(1), 210-214. https://doi.org/10.1006/jsre.1994.1133

Butyrate increases colonocyte protein synthesis in ulcerative colitis. / Frankel, W.; Lew, John; Su, B.; Bain, A.; Klurfeld, D.; Einhorn, E.; MacDermott, R. P.; Rombeau, J.

In: Journal of Surgical Research, Vol. 57, No. 1, 11.08.1994, p. 210-214.

Research output: Contribution to journalArticle

Frankel, W, Lew, J, Su, B, Bain, A, Klurfeld, D, Einhorn, E, MacDermott, RP & Rombeau, J 1994, 'Butyrate increases colonocyte protein synthesis in ulcerative colitis', Journal of Surgical Research, vol. 57, no. 1, pp. 210-214. https://doi.org/10.1006/jsre.1994.1133
Frankel, W. ; Lew, John ; Su, B. ; Bain, A. ; Klurfeld, D. ; Einhorn, E. ; MacDermott, R. P. ; Rombeau, J. / Butyrate increases colonocyte protein synthesis in ulcerative colitis. In: Journal of Surgical Research. 1994 ; Vol. 57, No. 1. pp. 210-214.
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