Bulbar conjunctival microvascular responses in dry eye

Wan Chen, Hatim Ismail M Batawi, Jimmy R. Alava, Anat Galor, Jin Yuan, Konstantinos D. Sarantopoulos, Allison L. McClellan, William J Feuer, Roy C Levitt, Jianhua Wang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Purpose Conjunctival microvascular responses may be a surrogate metric of efferent neural pathway function innervating the ocular surface as changes in blood flow occur within seconds after a stimulus. As somatosensory dysfunction may partially underlie dry eye (DE), in this study we evaluate whether bulbar conjunctival microvascular alterations correlate with various aspects of DE. Methods Fifty-six DE patients were prospectively recruited from a Veterans Affairs ophthalmology clinic over an 11-month period. DE symptoms and ocular pain were assessed along with DE signs. A novel functional slit lamp biomicroscope (FSLB) was used to image the temporal bulbar conjunctiva from the right eye before and after central corneal stimulation with an air puff. Blood flow velocities were measured and noninvasive microvascular perfusion maps (nMPMs) were created. Results The bulbar blood flow velocity was 0.50 ± 0.15 mm/s at baseline and increased to 0.55 ± 0.17 mm/s after stimulation (P < 0.001); the average change in velocity was 0.05 ± 0.09. nMPMs values and venule diameter, on the other hand, did not significantly increase after stimulation (1.64 ± 0.004 at baseline, 1.65 ± 0.04 after stimulation, P = 0.22 and 22.13 ± 1.84 μm at baseline, 22.21 ± 2.04 μm after stimulation, P = 0.73, respectively). Baseline blood flow velocity positively associated with Schirmer scores (r = 0.40, P = 0.002). Those with higher self-rated wind hyperalgesia demonstrated less change in blood flow velocity (r = −0.268, P = 0.046) after air stimulation on the central cornea. Conclusion Conjunctival blood flow velocity, but not vessel diameter or complexity, increases after wind stimuli. Baseline flow positively correlated with Schirmer scores while change in flow negatively correlated with self-reported wind hyperalgesia.

Original languageEnglish (US)
Pages (from-to)193-201
Number of pages9
JournalOcular Surface
Volume15
Issue number2
DOIs
StatePublished - Apr 1 2017

Fingerprint

Blood Flow Velocity
Eye Pain
Hyperalgesia
Perfusion
Air
Efferent Pathways
Neural Pathways
Venules
Conjunctiva
Veterans
Ophthalmology
Cornea

Keywords

  • Blood flow velocity
  • Corneal sensitivity
  • Fractal dimension
  • Functional slit lamp biomicroscopy
  • Neuropathic ocular pain

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Bulbar conjunctival microvascular responses in dry eye. / Chen, Wan; Batawi, Hatim Ismail M; Alava, Jimmy R.; Galor, Anat; Yuan, Jin; Sarantopoulos, Konstantinos D.; McClellan, Allison L.; Feuer, William J; Levitt, Roy C; Wang, Jianhua.

In: Ocular Surface, Vol. 15, No. 2, 01.04.2017, p. 193-201.

Research output: Contribution to journalArticle

Chen, W, Batawi, HIM, Alava, JR, Galor, A, Yuan, J, Sarantopoulos, KD, McClellan, AL, Feuer, WJ, Levitt, RC & Wang, J 2017, 'Bulbar conjunctival microvascular responses in dry eye', Ocular Surface, vol. 15, no. 2, pp. 193-201. https://doi.org/10.1016/j.jtos.2016.12.002
Chen, Wan ; Batawi, Hatim Ismail M ; Alava, Jimmy R. ; Galor, Anat ; Yuan, Jin ; Sarantopoulos, Konstantinos D. ; McClellan, Allison L. ; Feuer, William J ; Levitt, Roy C ; Wang, Jianhua. / Bulbar conjunctival microvascular responses in dry eye. In: Ocular Surface. 2017 ; Vol. 15, No. 2. pp. 193-201.
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AU - Alava, Jimmy R.

AU - Galor, Anat

AU - Yuan, Jin

AU - Sarantopoulos, Konstantinos D.

AU - McClellan, Allison L.

AU - Feuer, William J

AU - Levitt, Roy C

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N2 - Purpose Conjunctival microvascular responses may be a surrogate metric of efferent neural pathway function innervating the ocular surface as changes in blood flow occur within seconds after a stimulus. As somatosensory dysfunction may partially underlie dry eye (DE), in this study we evaluate whether bulbar conjunctival microvascular alterations correlate with various aspects of DE. Methods Fifty-six DE patients were prospectively recruited from a Veterans Affairs ophthalmology clinic over an 11-month period. DE symptoms and ocular pain were assessed along with DE signs. A novel functional slit lamp biomicroscope (FSLB) was used to image the temporal bulbar conjunctiva from the right eye before and after central corneal stimulation with an air puff. Blood flow velocities were measured and noninvasive microvascular perfusion maps (nMPMs) were created. Results The bulbar blood flow velocity was 0.50 ± 0.15 mm/s at baseline and increased to 0.55 ± 0.17 mm/s after stimulation (P < 0.001); the average change in velocity was 0.05 ± 0.09. nMPMs values and venule diameter, on the other hand, did not significantly increase after stimulation (1.64 ± 0.004 at baseline, 1.65 ± 0.04 after stimulation, P = 0.22 and 22.13 ± 1.84 μm at baseline, 22.21 ± 2.04 μm after stimulation, P = 0.73, respectively). Baseline blood flow velocity positively associated with Schirmer scores (r = 0.40, P = 0.002). Those with higher self-rated wind hyperalgesia demonstrated less change in blood flow velocity (r = −0.268, P = 0.046) after air stimulation on the central cornea. Conclusion Conjunctival blood flow velocity, but not vessel diameter or complexity, increases after wind stimuli. Baseline flow positively correlated with Schirmer scores while change in flow negatively correlated with self-reported wind hyperalgesia.

AB - Purpose Conjunctival microvascular responses may be a surrogate metric of efferent neural pathway function innervating the ocular surface as changes in blood flow occur within seconds after a stimulus. As somatosensory dysfunction may partially underlie dry eye (DE), in this study we evaluate whether bulbar conjunctival microvascular alterations correlate with various aspects of DE. Methods Fifty-six DE patients were prospectively recruited from a Veterans Affairs ophthalmology clinic over an 11-month period. DE symptoms and ocular pain were assessed along with DE signs. A novel functional slit lamp biomicroscope (FSLB) was used to image the temporal bulbar conjunctiva from the right eye before and after central corneal stimulation with an air puff. Blood flow velocities were measured and noninvasive microvascular perfusion maps (nMPMs) were created. Results The bulbar blood flow velocity was 0.50 ± 0.15 mm/s at baseline and increased to 0.55 ± 0.17 mm/s after stimulation (P < 0.001); the average change in velocity was 0.05 ± 0.09. nMPMs values and venule diameter, on the other hand, did not significantly increase after stimulation (1.64 ± 0.004 at baseline, 1.65 ± 0.04 after stimulation, P = 0.22 and 22.13 ± 1.84 μm at baseline, 22.21 ± 2.04 μm after stimulation, P = 0.73, respectively). Baseline blood flow velocity positively associated with Schirmer scores (r = 0.40, P = 0.002). Those with higher self-rated wind hyperalgesia demonstrated less change in blood flow velocity (r = −0.268, P = 0.046) after air stimulation on the central cornea. Conclusion Conjunctival blood flow velocity, but not vessel diameter or complexity, increases after wind stimuli. Baseline flow positively correlated with Schirmer scores while change in flow negatively correlated with self-reported wind hyperalgesia.

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