Bubbles and computer-aided image analysis for evaluation of surfactant inhibition

P. Berggren, J. Eklind, B. Linderholm, B. Robertson

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Surfactant (Curosurf, diluted to 5 mg/ml) was suspended in normal saline with 3 mM CaCl2 and mixed with different concentrations of albumin or fibrinogen. The samples were vortexed to generate microbubbles, and the equivalent circle diameter of these bubbles was determined with computer-aided image analysis. Bubbles in the original surfactant suspension had an average diameter of 27 μm after 2min, and their size increased only little during a 15-min period of observation. The diameter of bubbles generated in the presence of albumin (4 or 40 mg/ml) or fibrinogen (4 mg/ml) was 4-5 times larger, indicating surfactant inhibition. We conclude that evaluation of microbubble stability with image analysis provides an objective and rapid assessment of surfactant inhibition, and we speculate that the method could also be used for quantification of surfactant activity in airway samples.

Original languageEnglish
Pages (from-to)15-20
Number of pages6
JournalBiology of the Neonate
Volume61
Issue numberSUPPL. 1
StatePublished - Jan 1 1992
Externally publishedYes

Fingerprint

Surface-Active Agents
Microbubbles
Fibrinogen
Albumins
Suspensions
Observation

Keywords

  • Image analysis
  • Proteins
  • Pulmonary surfactants

ASJC Scopus subject areas

  • Developmental Biology
  • Pediatrics, Perinatology, and Child Health

Cite this

Berggren, P., Eklind, J., Linderholm, B., & Robertson, B. (1992). Bubbles and computer-aided image analysis for evaluation of surfactant inhibition. Biology of the Neonate, 61(SUPPL. 1), 15-20.

Bubbles and computer-aided image analysis for evaluation of surfactant inhibition. / Berggren, P.; Eklind, J.; Linderholm, B.; Robertson, B.

In: Biology of the Neonate, Vol. 61, No. SUPPL. 1, 01.01.1992, p. 15-20.

Research output: Contribution to journalArticle

Berggren, P, Eklind, J, Linderholm, B & Robertson, B 1992, 'Bubbles and computer-aided image analysis for evaluation of surfactant inhibition', Biology of the Neonate, vol. 61, no. SUPPL. 1, pp. 15-20.
Berggren P, Eklind J, Linderholm B, Robertson B. Bubbles and computer-aided image analysis for evaluation of surfactant inhibition. Biology of the Neonate. 1992 Jan 1;61(SUPPL. 1):15-20.
Berggren, P. ; Eklind, J. ; Linderholm, B. ; Robertson, B. / Bubbles and computer-aided image analysis for evaluation of surfactant inhibition. In: Biology of the Neonate. 1992 ; Vol. 61, No. SUPPL. 1. pp. 15-20.
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