Abstract
To extend our recent observation that villin mRNA, encoding an apical microvillous protein, is dichotomously localized in the basal region of human enterocytes, we examined the localization of mRNAs for brush border myosin I (BBMI) and intestinal timbrin (I-fim). In situ hybridization indicated that BBMI mRNA localized to the basal region of human enterocytes, whereas the mRNA for I-fim distributed diffusely. To facilitate study of potential mechanisms of mRNA targeting, we cloned a full-length cDNA for BBMI including its 5'- and 3'-untranslated regions (UTRs). This cDNA shares 86% sequence identity with bovine BBMI and 85% with rat BBMI. Sequence analysis revealed no obvious similarity between the 3'-UTRs of BBMI and villin. This study provides evidence of novel sorting pathways for intestinal microvillous cytoskeletal proteins.
Original language | English (US) |
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Pages (from-to) | 89-94 |
Number of pages | 6 |
Journal | Journal of Histochemistry and Cytochemistry |
Volume | 48 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2000 |
Externally published | Yes |
Keywords
- Brush border myosin I, BBMI
- Enterocyte
- I-fimbrin (I- plastin)
- Intestinal fimbrin
- mRNA sorting
ASJC Scopus subject areas
- Anatomy
- Histology