Brush border myosin I (BBMI): A basally localized transcript in human jejunal enterocytes

Wei Li, Jiyi Wang, Lynne M. Coluccio, Paul Matsudaira, Richard J. Grand

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

To extend our recent observation that villin mRNA, encoding an apical microvillous protein, is dichotomously localized in the basal region of human enterocytes, we examined the localization of mRNAs for brush border myosin I (BBMI) and intestinal timbrin (I-fim). In situ hybridization indicated that BBMI mRNA localized to the basal region of human enterocytes, whereas the mRNA for I-fim distributed diffusely. To facilitate study of potential mechanisms of mRNA targeting, we cloned a full-length cDNA for BBMI including its 5'- and 3'-untranslated regions (UTRs). This cDNA shares 86% sequence identity with bovine BBMI and 85% with rat BBMI. Sequence analysis revealed no obvious similarity between the 3'-UTRs of BBMI and villin. This study provides evidence of novel sorting pathways for intestinal microvillous cytoskeletal proteins.

Original languageEnglish (US)
Pages (from-to)89-94
Number of pages6
JournalJournal of Histochemistry and Cytochemistry
Volume48
Issue number1
DOIs
StatePublished - Jan 2000

Keywords

  • Brush border myosin I, BBMI
  • Enterocyte
  • I-fimbrin (I- plastin)
  • Intestinal fimbrin
  • mRNA sorting

ASJC Scopus subject areas

  • Anatomy
  • Histology

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