Brush border myosin I (BBMI): A basally localized transcript in human jejunal enterocytes

Wei Li; Jiyi Wang; Lynne M. Coluccio; Paul Matsudaira; Richard J. Grand

doi:10.1177/002215540004800109

Brush border myosin I (BBMI): A basally localized transcript in human jejunal enterocytes

Wei Li, Jiyi Wang, Lynne M. Coluccio, Paul Matsudaira, Richard J. Grand

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

To extend our recent observation that villin mRNA, encoding an apical microvillous protein, is dichotomously localized in the basal region of human enterocytes, we examined the localization of mRNAs for brush border myosin I (BBMI) and intestinal timbrin (I-fim). In situ hybridization indicated that BBMI mRNA localized to the basal region of human enterocytes, whereas the mRNA for I-fim distributed diffusely. To facilitate study of potential mechanisms of mRNA targeting, we cloned a full-length cDNA for BBMI including its 5'- and 3'-untranslated regions (UTRs). This cDNA shares 86% sequence identity with bovine BBMI and 85% with rat BBMI. Sequence analysis revealed no obvious similarity between the 3'-UTRs of BBMI and villin. This study provides evidence of novel sorting pathways for intestinal microvillous cytoskeletal proteins.

Original language	English (US)
Pages (from-to)	89-94
Number of pages	6
Journal	Journal of Histochemistry and Cytochemistry
Volume	48
Issue number	1
DOIs	https://doi.org/10.1177/002215540004800109
State	Published - Jan 2000
Externally published	Yes

Keywords

Brush border myosin I, BBMI
Enterocyte
I-fimbrin (I- plastin)
Intestinal fimbrin
mRNA sorting

ASJC Scopus subject areas

Anatomy
Histology

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title = "Brush border myosin I (BBMI): A basally localized transcript in human jejunal enterocytes",

abstract = "To extend our recent observation that villin mRNA, encoding an apical microvillous protein, is dichotomously localized in the basal region of human enterocytes, we examined the localization of mRNAs for brush border myosin I (BBMI) and intestinal timbrin (I-fim). In situ hybridization indicated that BBMI mRNA localized to the basal region of human enterocytes, whereas the mRNA for I-fim distributed diffusely. To facilitate study of potential mechanisms of mRNA targeting, we cloned a full-length cDNA for BBMI including its 5'- and 3'-untranslated regions (UTRs). This cDNA shares 86% sequence identity with bovine BBMI and 85% with rat BBMI. Sequence analysis revealed no obvious similarity between the 3'-UTRs of BBMI and villin. This study provides evidence of novel sorting pathways for intestinal microvillous cytoskeletal proteins.",

keywords = "Brush border myosin I, BBMI, Enterocyte, I-fimbrin (I- plastin), Intestinal fimbrin, mRNA sorting",

author = "Wei Li and Jiyi Wang and Coluccio, {Lynne M.} and Paul Matsudaira and Grand, {Richard J.}",

year = "2000",

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doi = "10.1177/002215540004800109",

language = "English (US)",

volume = "48",

pages = "89--94",

journal = "Journal of Histochemistry and Cytochemistry",

issn = "0022-1554",

publisher = "Histochemical Society Inc.",

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TY - JOUR

T1 - Brush border myosin I (BBMI)

T2 - A basally localized transcript in human jejunal enterocytes

AU - Li, Wei

AU - Wang, Jiyi

AU - Coluccio, Lynne M.

AU - Matsudaira, Paul

AU - Grand, Richard J.

PY - 2000/1

Y1 - 2000/1

N2 - To extend our recent observation that villin mRNA, encoding an apical microvillous protein, is dichotomously localized in the basal region of human enterocytes, we examined the localization of mRNAs for brush border myosin I (BBMI) and intestinal timbrin (I-fim). In situ hybridization indicated that BBMI mRNA localized to the basal region of human enterocytes, whereas the mRNA for I-fim distributed diffusely. To facilitate study of potential mechanisms of mRNA targeting, we cloned a full-length cDNA for BBMI including its 5'- and 3'-untranslated regions (UTRs). This cDNA shares 86% sequence identity with bovine BBMI and 85% with rat BBMI. Sequence analysis revealed no obvious similarity between the 3'-UTRs of BBMI and villin. This study provides evidence of novel sorting pathways for intestinal microvillous cytoskeletal proteins.

AB - To extend our recent observation that villin mRNA, encoding an apical microvillous protein, is dichotomously localized in the basal region of human enterocytes, we examined the localization of mRNAs for brush border myosin I (BBMI) and intestinal timbrin (I-fim). In situ hybridization indicated that BBMI mRNA localized to the basal region of human enterocytes, whereas the mRNA for I-fim distributed diffusely. To facilitate study of potential mechanisms of mRNA targeting, we cloned a full-length cDNA for BBMI including its 5'- and 3'-untranslated regions (UTRs). This cDNA shares 86% sequence identity with bovine BBMI and 85% with rat BBMI. Sequence analysis revealed no obvious similarity between the 3'-UTRs of BBMI and villin. This study provides evidence of novel sorting pathways for intestinal microvillous cytoskeletal proteins.

KW - Brush border myosin I, BBMI

KW - Enterocyte

KW - I-fimbrin (I- plastin)

KW - Intestinal fimbrin

KW - mRNA sorting

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JO - Journal of Histochemistry and Cytochemistry

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ER -

Brush border myosin I (BBMI): A basally localized transcript in human jejunal enterocytes

Abstract

Keywords

ASJC Scopus subject areas

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