Bronchial tissue kallikrein activity is regulated by hyaluronic acid binding

Rosanna M. Forteza, Isabel Lauredo, William M. Abraham, Gregory E Conner

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Tissue kallikrein (TK) is secreted by serous cells of tracheobronchial submucosal glands and plays a role in allergic airway responses. To better understand the regulation of TK, we used primary cultures of submucosal gland cells that release TK upon stimulation. Media from cultures stimulated with chymase (10-7 M) showed increased TK activity (0.50 ± 0.22 mU/ml mean ± standard error) in comparison with the control group (0.08 ± 0.02 mU/ml). The increased TK activity was significantly correlated with increases in the levels of the serous cell marker, secretory leukoprotease inhibitor. Anion exchange chromatography of the conditioned culture media showed that TK activity eluted as a broad peak between 1.6 and 1.8 M NaCl, unlike the reported elution (0.3 to 0.6 M NaCl) of kallikreins from other tissues, suggesting that secreted bronchial TK was bound to a negatively charged molecule. Hyaluronidase digestion increased TK activity in both pre-and post-chymase-stimulated culture media, whereas no such change was seen after samples were digested with heparinase or chondroitinase ABC. Further, after hyaluronidase digestion of media, TK eluted from an anion exchange column between 0.3 and 0.6 M NaCl. Enzymatic detection of TK after nondenaturing gel electrophoresis showed that hyaluronidase digestion also reduced the electrophoretic heterogeneity of TK to a single band, whereas adding back hyaluronic acid (HA) to hyaluronidase-digested samples restored the original heterogeneity. Finally, TK activity bound to HA-Sepharose and could be eluted with HA. These studies show that primary cultures of ovine submucosal gland cells secrete TK in a regulated fashion, and that secreted TK binds to HA. This binding reduces TK enzymatic activity; therefore, factors that affect HA turnover could modify the TK activity in the airway lumen. These events could be important in the regulation of kinin-mediated airway inflammation.

Original languageEnglish
Pages (from-to)666-674
Number of pages9
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume21
Issue number6
StatePublished - Dec 1 1999
Externally publishedYes

Fingerprint

Tissue Kallikreins
Hyaluronic Acid
Hyaluronoglucosaminidase
Chymases
Digestion
Anions
Culture Media
Secretory Leukocyte Peptidase Inhibitor
Heparin Lyase
Chondroitin ABC Lyase
Kinins
Kallikreins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Pulmonary and Respiratory Medicine

Cite this

Bronchial tissue kallikrein activity is regulated by hyaluronic acid binding. / Forteza, Rosanna M.; Lauredo, Isabel; Abraham, William M.; Conner, Gregory E.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 21, No. 6, 01.12.1999, p. 666-674.

Research output: Contribution to journalArticle

Forteza, Rosanna M. ; Lauredo, Isabel ; Abraham, William M. ; Conner, Gregory E. / Bronchial tissue kallikrein activity is regulated by hyaluronic acid binding. In: American Journal of Respiratory Cell and Molecular Biology. 1999 ; Vol. 21, No. 6. pp. 666-674.
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abstract = "Tissue kallikrein (TK) is secreted by serous cells of tracheobronchial submucosal glands and plays a role in allergic airway responses. To better understand the regulation of TK, we used primary cultures of submucosal gland cells that release TK upon stimulation. Media from cultures stimulated with chymase (10-7 M) showed increased TK activity (0.50 ± 0.22 mU/ml mean ± standard error) in comparison with the control group (0.08 ± 0.02 mU/ml). The increased TK activity was significantly correlated with increases in the levels of the serous cell marker, secretory leukoprotease inhibitor. Anion exchange chromatography of the conditioned culture media showed that TK activity eluted as a broad peak between 1.6 and 1.8 M NaCl, unlike the reported elution (0.3 to 0.6 M NaCl) of kallikreins from other tissues, suggesting that secreted bronchial TK was bound to a negatively charged molecule. Hyaluronidase digestion increased TK activity in both pre-and post-chymase-stimulated culture media, whereas no such change was seen after samples were digested with heparinase or chondroitinase ABC. Further, after hyaluronidase digestion of media, TK eluted from an anion exchange column between 0.3 and 0.6 M NaCl. Enzymatic detection of TK after nondenaturing gel electrophoresis showed that hyaluronidase digestion also reduced the electrophoretic heterogeneity of TK to a single band, whereas adding back hyaluronic acid (HA) to hyaluronidase-digested samples restored the original heterogeneity. Finally, TK activity bound to HA-Sepharose and could be eluted with HA. These studies show that primary cultures of ovine submucosal gland cells secrete TK in a regulated fashion, and that secreted TK binds to HA. This binding reduces TK enzymatic activity; therefore, factors that affect HA turnover could modify the TK activity in the airway lumen. These events could be important in the regulation of kinin-mediated airway inflammation.",
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