TY - JOUR
T1 - Brevetoxin PbTx-2 immunology
T2 - Differential epitope recognition by antibodies from two goats
AU - Melinek, Rosemary
AU - Rein, Kathleen S.
AU - Schultz, Duane R.
AU - Baden, Daniel G.
N1 - Funding Information:
Acknowkdgemaits-NIEHS grant # ROl ES05853 is gratefully acknowledged as a source offinancial support. Portions of these studies were undertaken es part of the University of Miami NIEHS Marine and Freshwater Biomedical Sciences Center program (P30 ES05705). Kwr~~r S. R~ thanks the National Institutes of Health for NRSA postdoctoral fellowship (F32 ES055667).
PY - 1994/8
Y1 - 1994/8
N2 - The epitopic regions of the brevetoxin PbTx-3 molecule, produced by the marine dinoflagellate Ptychodiscus brevis, have been identified by structural modification at three distinct regions of the toxin. These are: the A-ring lactone region of the molecule, the K-ring side-chain and the H-ring. The modified PbTx-3 derivatives were tested for their ability to bind brevetoxin goat antisera directed against the PbTx-3 molecule, by radio-immunoassay. The results showed that at least two major epitopes and one minor epitope are recognized: the A-ring lactone region of the molecule and the K-ring side-chain, and the H-ring. The results illustrate the variety of antibodies which may be produced, even within a species, and suggests that epitope characterization is important in the development of assays which are to be employed in seafood safety issues.
AB - The epitopic regions of the brevetoxin PbTx-3 molecule, produced by the marine dinoflagellate Ptychodiscus brevis, have been identified by structural modification at three distinct regions of the toxin. These are: the A-ring lactone region of the molecule, the K-ring side-chain and the H-ring. The modified PbTx-3 derivatives were tested for their ability to bind brevetoxin goat antisera directed against the PbTx-3 molecule, by radio-immunoassay. The results showed that at least two major epitopes and one minor epitope are recognized: the A-ring lactone region of the molecule and the K-ring side-chain, and the H-ring. The results illustrate the variety of antibodies which may be produced, even within a species, and suggests that epitope characterization is important in the development of assays which are to be employed in seafood safety issues.
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U2 - 10.1016/0041-0101(94)90367-0
DO - 10.1016/0041-0101(94)90367-0
M3 - Article
C2 - 7527163
AN - SCOPUS:0028102158
VL - 32
SP - 883
EP - 890
JO - Toxicon
JF - Toxicon
SN - 0041-0101
IS - 8
ER -