Abstract
The epitopic regions of the brevetoxin PbTx-3 molecule, produced by the marine dinoflagellate Ptychodiscus brevis, have been identified by structural modification at three distinct regions of the toxin. These are: the A-ring lactone region of the molecule, the K-ring side-chain and the H-ring. The modified PbTx-3 derivatives were tested for their ability to bind brevetoxin goat antisera directed against the PbTx-3 molecule, by radio-immunoassay. The results showed that at least two major epitopes and one minor epitope are recognized: the A-ring lactone region of the molecule and the K-ring side-chain, and the H-ring. The results illustrate the variety of antibodies which may be produced, even within a species, and suggests that epitope characterization is important in the development of assays which are to be employed in seafood safety issues.
| Original language | English |
|---|---|
| Pages (from-to) | 883-890 |
| Number of pages | 8 |
| Journal | Toxicon |
| Volume | 32 |
| Issue number | 8 |
| DOIs | |
| State | Published - Jan 1 1994 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Toxicology
Cite this
Brevetoxin PbTx-2 immunology : Differential epitope recognition by antibodies from two goats. / Melinek, Rosemary; Rein, Kathleen S.; Schultz, Duane R.; Baden, Daniel G.
In: Toxicon, Vol. 32, No. 8, 01.01.1994, p. 883-890.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Brevetoxin PbTx-2 immunology
T2 - Differential epitope recognition by antibodies from two goats
AU - Melinek, Rosemary
AU - Rein, Kathleen S.
AU - Schultz, Duane R.
AU - Baden, Daniel G.
PY - 1994/1/1
Y1 - 1994/1/1
N2 - The epitopic regions of the brevetoxin PbTx-3 molecule, produced by the marine dinoflagellate Ptychodiscus brevis, have been identified by structural modification at three distinct regions of the toxin. These are: the A-ring lactone region of the molecule, the K-ring side-chain and the H-ring. The modified PbTx-3 derivatives were tested for their ability to bind brevetoxin goat antisera directed against the PbTx-3 molecule, by radio-immunoassay. The results showed that at least two major epitopes and one minor epitope are recognized: the A-ring lactone region of the molecule and the K-ring side-chain, and the H-ring. The results illustrate the variety of antibodies which may be produced, even within a species, and suggests that epitope characterization is important in the development of assays which are to be employed in seafood safety issues.
AB - The epitopic regions of the brevetoxin PbTx-3 molecule, produced by the marine dinoflagellate Ptychodiscus brevis, have been identified by structural modification at three distinct regions of the toxin. These are: the A-ring lactone region of the molecule, the K-ring side-chain and the H-ring. The modified PbTx-3 derivatives were tested for their ability to bind brevetoxin goat antisera directed against the PbTx-3 molecule, by radio-immunoassay. The results showed that at least two major epitopes and one minor epitope are recognized: the A-ring lactone region of the molecule and the K-ring side-chain, and the H-ring. The results illustrate the variety of antibodies which may be produced, even within a species, and suggests that epitope characterization is important in the development of assays which are to be employed in seafood safety issues.
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UR - http://www.scopus.com/inward/citedby.url?scp=0028102158&partnerID=8YFLogxK
U2 - 10.1016/0041-0101(94)90367-0
DO - 10.1016/0041-0101(94)90367-0
M3 - Article
VL - 32
SP - 883
EP - 890
JO - Toxicon
JF - Toxicon
SN - 0041-0101
IS - 8
ER -