BRCA1 is associated with a human SWI/SNF-related complex: Linking chromatin remodeling to breast cancer

Daniel A. Bochar, Lai Wang, Hideo Beniya, Alexander Kinev, Yutong Xue, William S. Lane, Weidong Wang, Fatah Kashanchi, Ramin Shiekhattar

Research output: Contribution to journalArticle

411 Citations (Scopus)

Abstract

Germline mutations in the tumor suppressor gene, BRCA1, predispose individuals to breast and ovarian cancers. Using a combination of affinity- and conventional chromatographic techniques, we have isolated a predominant form of a multiprotein BRCA1-containing complex from human ceils displaying chromatin-remodeling activity. Mass spectrometric sequencing of components of this complex indicated that BRCA1 is associated with a SWI/SNF-related complex. We show that BRCA1 can directly interact with the BRG1 subunit of the SWI/SNF complex. Moreover, p53-mediated stimulation of transcription by BRCA1 was completely abrogated by either a dominant-negative mutant of BRG1 or the cancer-causing deletion in exon 11 of BRCA1. These findings reveal a direct function for BRCA1 in transcriptional control through modulation of chromatin structure.

Original languageEnglish (US)
Pages (from-to)257-265
Number of pages9
JournalCell
Volume102
Issue number2
StatePublished - Jul 21 2000
Externally publishedYes

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Chromatin Assembly and Disassembly
Germ-Line Mutation
Tumor Suppressor Genes
Ovarian Neoplasms
Chromatin
Exons
Breast Neoplasms
Transcription
Tumors
Neoplasms
Genes
Modulation

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Bochar, D. A., Wang, L., Beniya, H., Kinev, A., Xue, Y., Lane, W. S., ... Shiekhattar, R. (2000). BRCA1 is associated with a human SWI/SNF-related complex: Linking chromatin remodeling to breast cancer. Cell, 102(2), 257-265.

BRCA1 is associated with a human SWI/SNF-related complex : Linking chromatin remodeling to breast cancer. / Bochar, Daniel A.; Wang, Lai; Beniya, Hideo; Kinev, Alexander; Xue, Yutong; Lane, William S.; Wang, Weidong; Kashanchi, Fatah; Shiekhattar, Ramin.

In: Cell, Vol. 102, No. 2, 21.07.2000, p. 257-265.

Research output: Contribution to journalArticle

Bochar, DA, Wang, L, Beniya, H, Kinev, A, Xue, Y, Lane, WS, Wang, W, Kashanchi, F & Shiekhattar, R 2000, 'BRCA1 is associated with a human SWI/SNF-related complex: Linking chromatin remodeling to breast cancer', Cell, vol. 102, no. 2, pp. 257-265.
Bochar DA, Wang L, Beniya H, Kinev A, Xue Y, Lane WS et al. BRCA1 is associated with a human SWI/SNF-related complex: Linking chromatin remodeling to breast cancer. Cell. 2000 Jul 21;102(2):257-265.
Bochar, Daniel A. ; Wang, Lai ; Beniya, Hideo ; Kinev, Alexander ; Xue, Yutong ; Lane, William S. ; Wang, Weidong ; Kashanchi, Fatah ; Shiekhattar, Ramin. / BRCA1 is associated with a human SWI/SNF-related complex : Linking chromatin remodeling to breast cancer. In: Cell. 2000 ; Vol. 102, No. 2. pp. 257-265.
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