Brainstem projections to the phrenic nucleus: An anterograde and retrograde HRP study in the rabbit

Howard H. Ellenberger, Pedro L. Vera, James R. Haselton, Carole L. Haselton, Neil Schneiderman

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Brainstem projections to the phrenic nucleus were studied in rabbits using horseradish peroxidase conjugated with wheat germ agglutinin (WGA-HRP) as a retrograde and anterograde neuronal tracer. Injections of 1% WGA-HRP were centered in the phrenic nucleus in the C4-C5 ventral horn in 4 rabbits to identify pontomedullary nuclear groups that contain neurons projecting to the midcervical spinal cord. Regions of the rabbit brainstem that are homologous to the ventral respiratory group (VRG), dorsal respiratory group (DRG), Bötzinger Complex (BötC) and Kölliker-Fuse nucleus in the cat and rat were shown to provide the major pontomedullary projections to the phrenic nucleus. Injections of WGA-HRP into physiologically identified locations within DRG, VRG and BötC anterogradely labelled bulbospinal axons of these groups. These injections produced presumptive terminal labelling in the C4-C5 ventral horn in the region containing the phrenic cell column and the transverse phrenic motoneuron dendrite bundles as defined by WGA-HRP labelling of phrenic motoneurons. These results indicate: 1. 1) The presumptive excitatory (DRG, VRG) and inhibitory (BötC) bulbospinal control of phrenic motoneurons arise from the same medullary respiratory groups in the rabbit as in the cat and rat. 2. 2) The bulbospinal control of phrenic motoneurons is primarily via direct projections to the phrenic motor nucleus, and not through segmental propriospinal interneurons. 3. 3) As in the rat, the bulbospinal contribution of the DRG is less pronounced in the rabbit than in the cat. 4. 4) The rabbit and rat have a slight ipsilateral predominance in their bulbospinal projections to phrenic nucleus; whereas these projections have a contralateral predominance in the cat.

Original languageEnglish
Pages (from-to)163-174
Number of pages12
JournalBrain Research Bulletin
Volume24
Issue number2
DOIs
StatePublished - Jan 1 1990

Fingerprint

Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate
Brain Stem
Diaphragm
Motor Neurons
Rabbits
Cats
Horns
Injections
Wheat Germ Agglutinins
Interneurons
Horseradish Peroxidase
Dendrites
Axons
Cervical Cord
Spinal Cord
Neurons

Keywords

  • Bulbospinal
  • Horseradish peroxidase
  • Medulla
  • Neural control of respiration
  • Pons
  • Spinal cord

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Brainstem projections to the phrenic nucleus : An anterograde and retrograde HRP study in the rabbit. / Ellenberger, Howard H.; Vera, Pedro L.; Haselton, James R.; Haselton, Carole L.; Schneiderman, Neil.

In: Brain Research Bulletin, Vol. 24, No. 2, 01.01.1990, p. 163-174.

Research output: Contribution to journalArticle

Ellenberger, Howard H. ; Vera, Pedro L. ; Haselton, James R. ; Haselton, Carole L. ; Schneiderman, Neil. / Brainstem projections to the phrenic nucleus : An anterograde and retrograde HRP study in the rabbit. In: Brain Research Bulletin. 1990 ; Vol. 24, No. 2. pp. 163-174.
@article{f2915a4d7f954d36930ef4e8dd4bad9b,
title = "Brainstem projections to the phrenic nucleus: An anterograde and retrograde HRP study in the rabbit",
abstract = "Brainstem projections to the phrenic nucleus were studied in rabbits using horseradish peroxidase conjugated with wheat germ agglutinin (WGA-HRP) as a retrograde and anterograde neuronal tracer. Injections of 1{\%} WGA-HRP were centered in the phrenic nucleus in the C4-C5 ventral horn in 4 rabbits to identify pontomedullary nuclear groups that contain neurons projecting to the midcervical spinal cord. Regions of the rabbit brainstem that are homologous to the ventral respiratory group (VRG), dorsal respiratory group (DRG), B{\"o}tzinger Complex (B{\"o}tC) and K{\"o}lliker-Fuse nucleus in the cat and rat were shown to provide the major pontomedullary projections to the phrenic nucleus. Injections of WGA-HRP into physiologically identified locations within DRG, VRG and B{\"o}tC anterogradely labelled bulbospinal axons of these groups. These injections produced presumptive terminal labelling in the C4-C5 ventral horn in the region containing the phrenic cell column and the transverse phrenic motoneuron dendrite bundles as defined by WGA-HRP labelling of phrenic motoneurons. These results indicate: 1. 1) The presumptive excitatory (DRG, VRG) and inhibitory (B{\"o}tC) bulbospinal control of phrenic motoneurons arise from the same medullary respiratory groups in the rabbit as in the cat and rat. 2. 2) The bulbospinal control of phrenic motoneurons is primarily via direct projections to the phrenic motor nucleus, and not through segmental propriospinal interneurons. 3. 3) As in the rat, the bulbospinal contribution of the DRG is less pronounced in the rabbit than in the cat. 4. 4) The rabbit and rat have a slight ipsilateral predominance in their bulbospinal projections to phrenic nucleus; whereas these projections have a contralateral predominance in the cat.",
keywords = "Bulbospinal, Horseradish peroxidase, Medulla, Neural control of respiration, Pons, Spinal cord",
author = "Ellenberger, {Howard H.} and Vera, {Pedro L.} and Haselton, {James R.} and Haselton, {Carole L.} and Neil Schneiderman",
year = "1990",
month = "1",
day = "1",
doi = "10.1016/0361-9230(90)90201-A",
language = "English",
volume = "24",
pages = "163--174",
journal = "Brain Research Bulletin",
issn = "0361-9230",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Brainstem projections to the phrenic nucleus

T2 - An anterograde and retrograde HRP study in the rabbit

AU - Ellenberger, Howard H.

AU - Vera, Pedro L.

AU - Haselton, James R.

AU - Haselton, Carole L.

AU - Schneiderman, Neil

PY - 1990/1/1

Y1 - 1990/1/1

N2 - Brainstem projections to the phrenic nucleus were studied in rabbits using horseradish peroxidase conjugated with wheat germ agglutinin (WGA-HRP) as a retrograde and anterograde neuronal tracer. Injections of 1% WGA-HRP were centered in the phrenic nucleus in the C4-C5 ventral horn in 4 rabbits to identify pontomedullary nuclear groups that contain neurons projecting to the midcervical spinal cord. Regions of the rabbit brainstem that are homologous to the ventral respiratory group (VRG), dorsal respiratory group (DRG), Bötzinger Complex (BötC) and Kölliker-Fuse nucleus in the cat and rat were shown to provide the major pontomedullary projections to the phrenic nucleus. Injections of WGA-HRP into physiologically identified locations within DRG, VRG and BötC anterogradely labelled bulbospinal axons of these groups. These injections produced presumptive terminal labelling in the C4-C5 ventral horn in the region containing the phrenic cell column and the transverse phrenic motoneuron dendrite bundles as defined by WGA-HRP labelling of phrenic motoneurons. These results indicate: 1. 1) The presumptive excitatory (DRG, VRG) and inhibitory (BötC) bulbospinal control of phrenic motoneurons arise from the same medullary respiratory groups in the rabbit as in the cat and rat. 2. 2) The bulbospinal control of phrenic motoneurons is primarily via direct projections to the phrenic motor nucleus, and not through segmental propriospinal interneurons. 3. 3) As in the rat, the bulbospinal contribution of the DRG is less pronounced in the rabbit than in the cat. 4. 4) The rabbit and rat have a slight ipsilateral predominance in their bulbospinal projections to phrenic nucleus; whereas these projections have a contralateral predominance in the cat.

AB - Brainstem projections to the phrenic nucleus were studied in rabbits using horseradish peroxidase conjugated with wheat germ agglutinin (WGA-HRP) as a retrograde and anterograde neuronal tracer. Injections of 1% WGA-HRP were centered in the phrenic nucleus in the C4-C5 ventral horn in 4 rabbits to identify pontomedullary nuclear groups that contain neurons projecting to the midcervical spinal cord. Regions of the rabbit brainstem that are homologous to the ventral respiratory group (VRG), dorsal respiratory group (DRG), Bötzinger Complex (BötC) and Kölliker-Fuse nucleus in the cat and rat were shown to provide the major pontomedullary projections to the phrenic nucleus. Injections of WGA-HRP into physiologically identified locations within DRG, VRG and BötC anterogradely labelled bulbospinal axons of these groups. These injections produced presumptive terminal labelling in the C4-C5 ventral horn in the region containing the phrenic cell column and the transverse phrenic motoneuron dendrite bundles as defined by WGA-HRP labelling of phrenic motoneurons. These results indicate: 1. 1) The presumptive excitatory (DRG, VRG) and inhibitory (BötC) bulbospinal control of phrenic motoneurons arise from the same medullary respiratory groups in the rabbit as in the cat and rat. 2. 2) The bulbospinal control of phrenic motoneurons is primarily via direct projections to the phrenic motor nucleus, and not through segmental propriospinal interneurons. 3. 3) As in the rat, the bulbospinal contribution of the DRG is less pronounced in the rabbit than in the cat. 4. 4) The rabbit and rat have a slight ipsilateral predominance in their bulbospinal projections to phrenic nucleus; whereas these projections have a contralateral predominance in the cat.

KW - Bulbospinal

KW - Horseradish peroxidase

KW - Medulla

KW - Neural control of respiration

KW - Pons

KW - Spinal cord

UR - http://www.scopus.com/inward/record.url?scp=0025262372&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025262372&partnerID=8YFLogxK

U2 - 10.1016/0361-9230(90)90201-A

DO - 10.1016/0361-9230(90)90201-A

M3 - Article

C2 - 1691045

AN - SCOPUS:0025262372

VL - 24

SP - 163

EP - 174

JO - Brain Research Bulletin

JF - Brain Research Bulletin

SN - 0361-9230

IS - 2

ER -