Brain fibroblast growth factor. Nonidentity with myelin basic protein fragments

K. A. Thomas, M. C. Riley, Sandra Lemmon, N. C. Baglan, R. A. Bradshaw

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Fibroblast growth factor (FGF) from bovine brain has been reported to be a family of three polypeptide fragments derived by limited proteolysis from myelin basic protein (MBP) (Westall, F.C., Lennon, V.A., and Gospodarowicz, D. (1978) Proc. Natl. Acad. Sci. U.S.A. 75, 4675-4678). However, fragments of sequence similar to the proposed active ones, generated from bovine MBP) by acid proteases, are inactive in stimulating [ 3H]thymidine incorporation in BALB/c 3T3 cells. Further, the principal active component of the brain FGF preparation (Gospodarowicz, D., Bialecki, H., and Greenburg, G. (1978) J. Biol. Chem. 253, 3736-3743) which can be recovered in high yield from isoelectric focusing in sucrose has a pI between 4.8 and 5.8 in contradistinction to the MBP fragments (pI ~ 10) and is not retained on a column of chicken anti-bovine MBP-Sepharose. Therefore, although the reported preparation of brain FGF gives an increase in activity units/mg of protein of about 1000-fold over the crude brain extract, the main protein components, the MBP fragments, do not possess the mitogenic activity. Additional purification of as much as 50- to 100-fold may be required to obtain a homogeneous preparation of the real brain FGF.

Original languageEnglish
Pages (from-to)5517-5520
Number of pages4
JournalJournal of Biological Chemistry
Volume255
Issue number12
StatePublished - Dec 1 1980
Externally publishedYes

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Myelin Basic Protein
Fibroblast Growth Factors
Brain
BALB 3T3 Cells
Proteolysis
Isoelectric Focusing
Complex Mixtures
Sepharose
Thymidine
Purification
Sucrose
Chickens
Proteins
Peptide Hydrolases
Peptides
Acids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Thomas, K. A., Riley, M. C., Lemmon, S., Baglan, N. C., & Bradshaw, R. A. (1980). Brain fibroblast growth factor. Nonidentity with myelin basic protein fragments. Journal of Biological Chemistry, 255(12), 5517-5520.

Brain fibroblast growth factor. Nonidentity with myelin basic protein fragments. / Thomas, K. A.; Riley, M. C.; Lemmon, Sandra; Baglan, N. C.; Bradshaw, R. A.

In: Journal of Biological Chemistry, Vol. 255, No. 12, 01.12.1980, p. 5517-5520.

Research output: Contribution to journalArticle

Thomas, KA, Riley, MC, Lemmon, S, Baglan, NC & Bradshaw, RA 1980, 'Brain fibroblast growth factor. Nonidentity with myelin basic protein fragments', Journal of Biological Chemistry, vol. 255, no. 12, pp. 5517-5520.
Thomas, K. A. ; Riley, M. C. ; Lemmon, Sandra ; Baglan, N. C. ; Bradshaw, R. A. / Brain fibroblast growth factor. Nonidentity with myelin basic protein fragments. In: Journal of Biological Chemistry. 1980 ; Vol. 255, No. 12. pp. 5517-5520.
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