Brain expression of apolipoproteins E, J, and A-I in Alzheimer's disease

Steven D. Harr, Luciana Uint, Richard Hollister, Bradley T. Hyman, Armando J Mendez

Research output: Contribution to journalArticle

145 Citations (Scopus)

Abstract

Inheritance of the ε4 allele of apolipoprotein (apo) E is associated with increased risk of Alzheimer's disease (AD) and with increased β-amyloid peptide (Aβ) deposition in the cortex. Apo E is a member of a family of exchangeable apos, characterized by the presence of amphipathic α-helical segments that allow these molecules to act as surfactants on the surface of lipoprotein particles. Two members of this family, apo E and apo J, have been shown to bind soluble Aβ, and both are associated with senile plaques in the AD cortex. We now have studied the pattern of brain apo expression and found that five members of this class are present: apo A-I, A-IV, D, E, and J. By contrast, apos A-II, B, and C-II were not detectable. Immunohistochemistry revealed that, in addition to apo E and apo J, apo A-I immunostained occasional senile plaques in AD cortex. Immunoblot analysis showed no difference in the relative amounts of any of these apos in tissue homogenates of frontal lobe from AD or control patients. Comparison by APO E genotype showed no differences in the amount of apo E in brain among APO E ε3/3, ε3/4, or ε4/4 individuals; however, a significant decrease in the amount of apo J was associated with the APO E ε4 allele. No differences in apo J levels were detected in CSF samples of AD subjects. We propose that several members of the exchangeable apo family may interact with Aβ deposits in senile plaques through common amphipathic α-helical domains. Competition among these molecules for binding of Aβ or Aβ aggregates may influence the deposition of Aβ in senile plaques.

Original languageEnglish
Pages (from-to)2429-2435
Number of pages7
JournalJournal of Neurochemistry
Volume66
Issue number6
StatePublished - Jun 1 1996
Externally publishedYes

Fingerprint

Clusterin
Apolipoproteins A
Apolipoproteins E
Amyloid Plaques
Brain
Alzheimer Disease
Apolipoproteins
Apolipoprotein A-I
Alleles
Apolipoprotein E4
Molecules
Frontal Lobe
Amyloid
Surface-Active Agents
Lipoproteins
Deposits
Immunohistochemistry
Genotype
Tissue

Keywords

  • β-Amyloid peptide
  • Alzheimer's disease
  • Apolipoproteins
  • Senile plaques

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Harr, S. D., Uint, L., Hollister, R., Hyman, B. T., & Mendez, A. J. (1996). Brain expression of apolipoproteins E, J, and A-I in Alzheimer's disease. Journal of Neurochemistry, 66(6), 2429-2435.

Brain expression of apolipoproteins E, J, and A-I in Alzheimer's disease. / Harr, Steven D.; Uint, Luciana; Hollister, Richard; Hyman, Bradley T.; Mendez, Armando J.

In: Journal of Neurochemistry, Vol. 66, No. 6, 01.06.1996, p. 2429-2435.

Research output: Contribution to journalArticle

Harr, SD, Uint, L, Hollister, R, Hyman, BT & Mendez, AJ 1996, 'Brain expression of apolipoproteins E, J, and A-I in Alzheimer's disease', Journal of Neurochemistry, vol. 66, no. 6, pp. 2429-2435.
Harr, Steven D. ; Uint, Luciana ; Hollister, Richard ; Hyman, Bradley T. ; Mendez, Armando J. / Brain expression of apolipoproteins E, J, and A-I in Alzheimer's disease. In: Journal of Neurochemistry. 1996 ; Vol. 66, No. 6. pp. 2429-2435.
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