Brain development in mice lacking L1-L1 homophilic adhesion

Kyoko Itoh, Ling Cheng, Yoshimasa Kamei, Shinji Fushiki, Hiroyuki Kamiguchi, Paul Gutwein, Alexander Stoeck, Bernd Arnold, Peter Altevogt, Vance Lemmon

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

A new mouse line has been produced in which the sixth Ig domain of the L1 cell adhesion molecule has been deleted. Despite the rather large deletion, L1 expression is preserved at normal levels. In vitro experiments showed that L1-L1 homophilic binding was lost, along with L1-α5β1 integrin binding. However, L1-neurocan and L1-neuropilin binding were preserved and sema3a responses were intact. Surprisingly, many of the axon guidance defects present in the L1 knockout mice, such as abnormal corticospinal tract and corpus callosum, were not observed. Nonetheless, when backcrossed on the C57BL/ 6 strain, a severe hydrocephalus was observed and after several generations, became an embryonic lethal. These results imply that L1 binding to L1, TAG-1, or F3, and L1-α5β1 integrin binding are not essential for normal development of a variety of axon pathways, and suggest that L1-L1 homophilic binding is important in the production of X-linked hydrocephalus.

Original languageEnglish
Pages (from-to)145-154
Number of pages10
JournalJournal of Cell Biology
Volume165
Issue number1
DOIs
StatePublished - Apr 12 2004

Fingerprint

Integrins
Neurocan
Neuropilins
Semaphorin-3A
Neural Cell Adhesion Molecule L1
Pyramidal Tracts
Corpus Callosum
Brain
Hydrocephalus
Knockout Mice
Axons
Axon Guidance
In Vitro Techniques
X-linked Hydrocephalus
Immunoglobulin Domains

Keywords

  • Adhesion
  • Corticospinal tract
  • Hydrocephalus
  • Integrin
  • L1 cam

ASJC Scopus subject areas

  • Cell Biology

Cite this

Itoh, K., Cheng, L., Kamei, Y., Fushiki, S., Kamiguchi, H., Gutwein, P., ... Lemmon, V. (2004). Brain development in mice lacking L1-L1 homophilic adhesion. Journal of Cell Biology, 165(1), 145-154. https://doi.org/10.1083/jcb.200312107

Brain development in mice lacking L1-L1 homophilic adhesion. / Itoh, Kyoko; Cheng, Ling; Kamei, Yoshimasa; Fushiki, Shinji; Kamiguchi, Hiroyuki; Gutwein, Paul; Stoeck, Alexander; Arnold, Bernd; Altevogt, Peter; Lemmon, Vance.

In: Journal of Cell Biology, Vol. 165, No. 1, 12.04.2004, p. 145-154.

Research output: Contribution to journalArticle

Itoh, K, Cheng, L, Kamei, Y, Fushiki, S, Kamiguchi, H, Gutwein, P, Stoeck, A, Arnold, B, Altevogt, P & Lemmon, V 2004, 'Brain development in mice lacking L1-L1 homophilic adhesion', Journal of Cell Biology, vol. 165, no. 1, pp. 145-154. https://doi.org/10.1083/jcb.200312107
Itoh K, Cheng L, Kamei Y, Fushiki S, Kamiguchi H, Gutwein P et al. Brain development in mice lacking L1-L1 homophilic adhesion. Journal of Cell Biology. 2004 Apr 12;165(1):145-154. https://doi.org/10.1083/jcb.200312107
Itoh, Kyoko ; Cheng, Ling ; Kamei, Yoshimasa ; Fushiki, Shinji ; Kamiguchi, Hiroyuki ; Gutwein, Paul ; Stoeck, Alexander ; Arnold, Bernd ; Altevogt, Peter ; Lemmon, Vance. / Brain development in mice lacking L1-L1 homophilic adhesion. In: Journal of Cell Biology. 2004 ; Vol. 165, No. 1. pp. 145-154.
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