Brain-derived neurotrophic factor and neurotrophin-3 support the survival and neuritogenesis response of developing cochleovestibular ganglion neurons

Matias A. Avila, Isabel Varela-Nieto, Guillermo Romero, Jose M. Mato, Fernando Giraldez, Thomas R Van De Water, Juan Represa

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

The effects of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) on the differentiation of avian cochleovestibular ganglion and their possible association with the hydrolysis of glycosyl-phosphatidylinositol (GPI) were studied. BDNF and NT-3 (2 ng/ml) promoted neurite outgrowth in explants of both cochlear and vestibular ganglia. This effect on neuritogenesis was stage-dependent, reaching a maximum at E7 for NT-3 and at E9 for BDNF. The magnitude of the response of the vestibular ganglion to BDNF was always smaller than that of the cochlear ganglion of an equivalent stage. BDNF and NT-3 stimulation of neuronal survival and neurite extension was also demonstrated in dissociated neuronal cell cultures. The effect was concentration-dependent with saturation of the response occurring at 4 ng/ml for BDNF and at 2 ng/ml for NT-3, the half-maximal effect occurring at 2 and 1 ng/ml, respectively, for the most sensitive stages of the chick cochlear ganglion. Inositol phosphoglycan (IPG) did not mimic the effects of BDNF or NT-3 on neuronal survival and neurite outgrowth, nor was it able to potentiate their responses. Antibodies raised against IPG did not block the effects of these neurotrophins. The results suggest that BDNF and NT-3 may act in cooperation to establish the innervation pattern of the inner ear. Unlike their early proliferative effects, neurotrophic effects are uncoupled from the GPI/IPG signal transduction system.

Original languageEnglish
Pages (from-to)266-275
Number of pages10
JournalDevelopmental Biology
Volume159
Issue number1
StatePublished - Dec 1 1993
Externally publishedYes

Fingerprint

Neurotrophin 3
Brain-Derived Neurotrophic Factor
Ganglia
Neurons
Cochlea
Glycosylphosphatidylinositols
Nerve Growth Factors
Neurites
Inner Ear
Signal Transduction
Hydrolysis
Cell Culture Techniques
Antibodies

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Avila, M. A., Varela-Nieto, I., Romero, G., Mato, J. M., Giraldez, F., Van De Water, T. R., & Represa, J. (1993). Brain-derived neurotrophic factor and neurotrophin-3 support the survival and neuritogenesis response of developing cochleovestibular ganglion neurons. Developmental Biology, 159(1), 266-275.

Brain-derived neurotrophic factor and neurotrophin-3 support the survival and neuritogenesis response of developing cochleovestibular ganglion neurons. / Avila, Matias A.; Varela-Nieto, Isabel; Romero, Guillermo; Mato, Jose M.; Giraldez, Fernando; Van De Water, Thomas R; Represa, Juan.

In: Developmental Biology, Vol. 159, No. 1, 01.12.1993, p. 266-275.

Research output: Contribution to journalArticle

Avila, MA, Varela-Nieto, I, Romero, G, Mato, JM, Giraldez, F, Van De Water, TR & Represa, J 1993, 'Brain-derived neurotrophic factor and neurotrophin-3 support the survival and neuritogenesis response of developing cochleovestibular ganglion neurons', Developmental Biology, vol. 159, no. 1, pp. 266-275.
Avila, Matias A. ; Varela-Nieto, Isabel ; Romero, Guillermo ; Mato, Jose M. ; Giraldez, Fernando ; Van De Water, Thomas R ; Represa, Juan. / Brain-derived neurotrophic factor and neurotrophin-3 support the survival and neuritogenesis response of developing cochleovestibular ganglion neurons. In: Developmental Biology. 1993 ; Vol. 159, No. 1. pp. 266-275.
@article{d55bdb5e96e5401786c5994940390414,
title = "Brain-derived neurotrophic factor and neurotrophin-3 support the survival and neuritogenesis response of developing cochleovestibular ganglion neurons",
abstract = "The effects of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) on the differentiation of avian cochleovestibular ganglion and their possible association with the hydrolysis of glycosyl-phosphatidylinositol (GPI) were studied. BDNF and NT-3 (2 ng/ml) promoted neurite outgrowth in explants of both cochlear and vestibular ganglia. This effect on neuritogenesis was stage-dependent, reaching a maximum at E7 for NT-3 and at E9 for BDNF. The magnitude of the response of the vestibular ganglion to BDNF was always smaller than that of the cochlear ganglion of an equivalent stage. BDNF and NT-3 stimulation of neuronal survival and neurite extension was also demonstrated in dissociated neuronal cell cultures. The effect was concentration-dependent with saturation of the response occurring at 4 ng/ml for BDNF and at 2 ng/ml for NT-3, the half-maximal effect occurring at 2 and 1 ng/ml, respectively, for the most sensitive stages of the chick cochlear ganglion. Inositol phosphoglycan (IPG) did not mimic the effects of BDNF or NT-3 on neuronal survival and neurite outgrowth, nor was it able to potentiate their responses. Antibodies raised against IPG did not block the effects of these neurotrophins. The results suggest that BDNF and NT-3 may act in cooperation to establish the innervation pattern of the inner ear. Unlike their early proliferative effects, neurotrophic effects are uncoupled from the GPI/IPG signal transduction system.",
author = "Avila, {Matias A.} and Isabel Varela-Nieto and Guillermo Romero and Mato, {Jose M.} and Fernando Giraldez and {Van De Water}, {Thomas R} and Juan Represa",
year = "1993",
month = "12",
day = "1",
language = "English",
volume = "159",
pages = "266--275",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Brain-derived neurotrophic factor and neurotrophin-3 support the survival and neuritogenesis response of developing cochleovestibular ganglion neurons

AU - Avila, Matias A.

AU - Varela-Nieto, Isabel

AU - Romero, Guillermo

AU - Mato, Jose M.

AU - Giraldez, Fernando

AU - Van De Water, Thomas R

AU - Represa, Juan

PY - 1993/12/1

Y1 - 1993/12/1

N2 - The effects of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) on the differentiation of avian cochleovestibular ganglion and their possible association with the hydrolysis of glycosyl-phosphatidylinositol (GPI) were studied. BDNF and NT-3 (2 ng/ml) promoted neurite outgrowth in explants of both cochlear and vestibular ganglia. This effect on neuritogenesis was stage-dependent, reaching a maximum at E7 for NT-3 and at E9 for BDNF. The magnitude of the response of the vestibular ganglion to BDNF was always smaller than that of the cochlear ganglion of an equivalent stage. BDNF and NT-3 stimulation of neuronal survival and neurite extension was also demonstrated in dissociated neuronal cell cultures. The effect was concentration-dependent with saturation of the response occurring at 4 ng/ml for BDNF and at 2 ng/ml for NT-3, the half-maximal effect occurring at 2 and 1 ng/ml, respectively, for the most sensitive stages of the chick cochlear ganglion. Inositol phosphoglycan (IPG) did not mimic the effects of BDNF or NT-3 on neuronal survival and neurite outgrowth, nor was it able to potentiate their responses. Antibodies raised against IPG did not block the effects of these neurotrophins. The results suggest that BDNF and NT-3 may act in cooperation to establish the innervation pattern of the inner ear. Unlike their early proliferative effects, neurotrophic effects are uncoupled from the GPI/IPG signal transduction system.

AB - The effects of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) on the differentiation of avian cochleovestibular ganglion and their possible association with the hydrolysis of glycosyl-phosphatidylinositol (GPI) were studied. BDNF and NT-3 (2 ng/ml) promoted neurite outgrowth in explants of both cochlear and vestibular ganglia. This effect on neuritogenesis was stage-dependent, reaching a maximum at E7 for NT-3 and at E9 for BDNF. The magnitude of the response of the vestibular ganglion to BDNF was always smaller than that of the cochlear ganglion of an equivalent stage. BDNF and NT-3 stimulation of neuronal survival and neurite extension was also demonstrated in dissociated neuronal cell cultures. The effect was concentration-dependent with saturation of the response occurring at 4 ng/ml for BDNF and at 2 ng/ml for NT-3, the half-maximal effect occurring at 2 and 1 ng/ml, respectively, for the most sensitive stages of the chick cochlear ganglion. Inositol phosphoglycan (IPG) did not mimic the effects of BDNF or NT-3 on neuronal survival and neurite outgrowth, nor was it able to potentiate their responses. Antibodies raised against IPG did not block the effects of these neurotrophins. The results suggest that BDNF and NT-3 may act in cooperation to establish the innervation pattern of the inner ear. Unlike their early proliferative effects, neurotrophic effects are uncoupled from the GPI/IPG signal transduction system.

UR - http://www.scopus.com/inward/record.url?scp=0027449436&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027449436&partnerID=8YFLogxK

M3 - Article

C2 - 8365565

AN - SCOPUS:0027449436

VL - 159

SP - 266

EP - 275

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -