Brain amino acid concentrations in rats killed by decapitation and microwave irradiation

Joanne M. Miller, Richard S Jope, Thomas N. Ferraro, Theodore A. Hare

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The effect of death by decapitation or focused beam microwave irradiation (FBMI) on rat brain amino acid concentrations was investigated. Twenty-nine amino acids and related compounds were measured by ion-exchange chromatography in the cerebral cortex, hippocampus, striatum and substantia nigra of male Sprague-Dawley rats killed by decapitation (n = 5) or by FBMI (n = 5). Alanine, GABA, ethanolamine and NH3 concentrations were significantly lower in all 4 brain regions of the FBMI group animals. Valine, leucine, tyrosine and phenylalanine levels were also lower in the hippocampus, striatum and substantia nigra of the FBMI group. The FBMI group showed less aspartate in the hippocampus and substantia nigra as well as less glycine in the cortex, hippocampus and striatum. In the FBMI group, the only amino acids exhibiting significantly higher levels were GSH in the striatum and substantia nigra and glutamate in the substantia nigra. These results show a significant impact of method of killing on the determination of baseline concentrations of brain amino acids.

Original languageEnglish
Pages (from-to)187-192
Number of pages6
JournalJournal of Neuroscience Methods
Volume31
Issue number3
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

Fingerprint

Decapitation
Microwaves
Substantia Nigra
Amino Acids
Hippocampus
Brain
Ethanolamine
Ion Exchange Chromatography
Valine
Phenylalanine
Aspartic Acid
Leucine
Alanine
Cerebral Cortex
Glycine
gamma-Aminobutyric Acid
Sprague Dawley Rats
Tyrosine
Glutamic Acid

Keywords

  • Amino acids
  • Microwave irradiation
  • Neurotransmitters
  • γ-Aminobutyric acid

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Brain amino acid concentrations in rats killed by decapitation and microwave irradiation. / Miller, Joanne M.; Jope, Richard S; Ferraro, Thomas N.; Hare, Theodore A.

In: Journal of Neuroscience Methods, Vol. 31, No. 3, 01.01.1990, p. 187-192.

Research output: Contribution to journalArticle

Miller, Joanne M. ; Jope, Richard S ; Ferraro, Thomas N. ; Hare, Theodore A. / Brain amino acid concentrations in rats killed by decapitation and microwave irradiation. In: Journal of Neuroscience Methods. 1990 ; Vol. 31, No. 3. pp. 187-192.
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