Bradykinin induces a B2 receptor-mediated calcium signal linked to prostanoid formation in human gingival fibroblasts in vitro

U. H. Lerner, G. Brunius, I. Anduren, P. O. Berggren, L. Juntti-Berggren, T. Modéer

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Abstract

The aim of the study was to determine the effect of bradykinin (BK) on the level of cytoplasmic-free Ca2+, [Ca2+]i, in human gingival fibroblasts and its relation to BK-induced prostanoid formation. BK, but not des-Arg9-BK, induced a significant rapid (within seconds) and transient increase in [Ca2+]i, that was not dependent on extracellular Ca2+. The stimulatory effect of BK was seen in concentrations at or above 10-8M, with the most pronounced effect at 10-6M. d-Arg0-Hyp3-Thi5,8-dPhe7-BK, a BK B2 receptor antagonist, but not des-Arg9-Leu8-BK, a BK B1 receptor antagonist, blocked BK-induced rise in [Ca2+]i. The BK B2 receptor antagonist also significantly reduced BK-induced PGE2 formation. When extracellular Ca2+ in the incubation medium was depleted, either by addition of EGTA or by omission of Ca2+ addition, BK still caused a significant stimulation of PGE2 formation. The calcium ionophores A23187 and ionomycin, similar to BK, caused a burst of PGE2 formation. The two phorbol esters phorbol 12,13-dibutyrate and 4-β-phorbol-didecanoate positively amplified calcium ionophore A23187-induced PGE2 formation. The results indicate that BK-induced PGE2 formation in gingival fibroblasts is coupled to an increase in [Ca2+]i mediated by the BK B2 receptor, and which is independent of extracellular Ca2+.

Original languageEnglish (US)
Pages (from-to)44-52
Number of pages9
JournalAgents and Actions
Volume37
Issue number1-2
DOIs
StatePublished - Sep 1 1992

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology (medical)

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    Lerner, U. H., Brunius, G., Anduren, I., Berggren, P. O., Juntti-Berggren, L., & Modéer, T. (1992). Bradykinin induces a B2 receptor-mediated calcium signal linked to prostanoid formation in human gingival fibroblasts in vitro. Agents and Actions, 37(1-2), 44-52. https://doi.org/10.1007/BF01987889