Abstract
The aim of the study was to determine the effect of bradykinin (BK) on the level of cytoplasmic-free Ca2+, [Ca2+]i, in human gingival fibroblasts and its relation to BK-induced prostanoid formation. BK, but not des-Arg9-BK, induced a significant rapid (within seconds) and transient increase in [Ca2+]i, that was not dependent on extracellular Ca2+. The stimulatory effect of BK was seen in concentrations at or above 10-8M, with the most pronounced effect at 10-6M. d-Arg0-Hyp3-Thi5,8-dPhe7-BK, a BK B2 receptor antagonist, but not des-Arg9-Leu8-BK, a BK B1 receptor antagonist, blocked BK-induced rise in [Ca2+]i. The BK B2 receptor antagonist also significantly reduced BK-induced PGE2 formation. When extracellular Ca2+ in the incubation medium was depleted, either by addition of EGTA or by omission of Ca2+ addition, BK still caused a significant stimulation of PGE2 formation. The calcium ionophores A23187 and ionomycin, similar to BK, caused a burst of PGE2 formation. The two phorbol esters phorbol 12,13-dibutyrate and 4-β-phorbol-didecanoate positively amplified calcium ionophore A23187-induced PGE2 formation. The results indicate that BK-induced PGE2 formation in gingival fibroblasts is coupled to an increase in [Ca2+]i mediated by the BK B2 receptor, and which is independent of extracellular Ca2+.
| Original language | English |
|---|---|
| Pages (from-to) | 44-52 |
| Number of pages | 9 |
| Journal | Agents and Actions |
| Volume | 37 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Sep 1 1992 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Toxicology
- Pharmacology (medical)
Cite this
Bradykinin induces a B2 receptor-mediated calcium signal linked to prostanoid formation in human gingival fibroblasts in vitro. / Lerner, U. H.; Brunius, G.; Anduren, I.; Berggren, P. O.; Juntti-Berggren, L.; Modéer, T.
In: Agents and Actions, Vol. 37, No. 1-2, 01.09.1992, p. 44-52.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Bradykinin induces a B2 receptor-mediated calcium signal linked to prostanoid formation in human gingival fibroblasts in vitro
AU - Lerner, U. H.
AU - Brunius, G.
AU - Anduren, I.
AU - Berggren, P. O.
AU - Juntti-Berggren, L.
AU - Modéer, T.
PY - 1992/9/1
Y1 - 1992/9/1
N2 - The aim of the study was to determine the effect of bradykinin (BK) on the level of cytoplasmic-free Ca2+, [Ca2+]i, in human gingival fibroblasts and its relation to BK-induced prostanoid formation. BK, but not des-Arg9-BK, induced a significant rapid (within seconds) and transient increase in [Ca2+]i, that was not dependent on extracellular Ca2+. The stimulatory effect of BK was seen in concentrations at or above 10-8M, with the most pronounced effect at 10-6M. d-Arg0-Hyp3-Thi5,8-dPhe7-BK, a BK B2 receptor antagonist, but not des-Arg9-Leu8-BK, a BK B1 receptor antagonist, blocked BK-induced rise in [Ca2+]i. The BK B2 receptor antagonist also significantly reduced BK-induced PGE2 formation. When extracellular Ca2+ in the incubation medium was depleted, either by addition of EGTA or by omission of Ca2+ addition, BK still caused a significant stimulation of PGE2 formation. The calcium ionophores A23187 and ionomycin, similar to BK, caused a burst of PGE2 formation. The two phorbol esters phorbol 12,13-dibutyrate and 4-β-phorbol-didecanoate positively amplified calcium ionophore A23187-induced PGE2 formation. The results indicate that BK-induced PGE2 formation in gingival fibroblasts is coupled to an increase in [Ca2+]i mediated by the BK B2 receptor, and which is independent of extracellular Ca2+.
AB - The aim of the study was to determine the effect of bradykinin (BK) on the level of cytoplasmic-free Ca2+, [Ca2+]i, in human gingival fibroblasts and its relation to BK-induced prostanoid formation. BK, but not des-Arg9-BK, induced a significant rapid (within seconds) and transient increase in [Ca2+]i, that was not dependent on extracellular Ca2+. The stimulatory effect of BK was seen in concentrations at or above 10-8M, with the most pronounced effect at 10-6M. d-Arg0-Hyp3-Thi5,8-dPhe7-BK, a BK B2 receptor antagonist, but not des-Arg9-Leu8-BK, a BK B1 receptor antagonist, blocked BK-induced rise in [Ca2+]i. The BK B2 receptor antagonist also significantly reduced BK-induced PGE2 formation. When extracellular Ca2+ in the incubation medium was depleted, either by addition of EGTA or by omission of Ca2+ addition, BK still caused a significant stimulation of PGE2 formation. The calcium ionophores A23187 and ionomycin, similar to BK, caused a burst of PGE2 formation. The two phorbol esters phorbol 12,13-dibutyrate and 4-β-phorbol-didecanoate positively amplified calcium ionophore A23187-induced PGE2 formation. The results indicate that BK-induced PGE2 formation in gingival fibroblasts is coupled to an increase in [Ca2+]i mediated by the BK B2 receptor, and which is independent of extracellular Ca2+.
UR - http://www.scopus.com/inward/record.url?scp=0026784214&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026784214&partnerID=8YFLogxK
U2 - 10.1007/BF01987889
DO - 10.1007/BF01987889
M3 - Article
C2 - 1333726
AN - SCOPUS:0026784214
VL - 37
SP - 44
EP - 52
JO - Inflammation Research
JF - Inflammation Research
SN - 1023-3830
IS - 1-2
ER -